CD31/PECAM-1 Antibody (MEC 7.46)

Images

 
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31 (MEC 7.46) antibody NB100-1642(blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM1 (MEC 7.46) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IP
Clone
MEC 7.46
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

CD31/PECAM-1 Antibody (MEC 7.46) Summary

Immunogen
mouse endothelial cell line T-end
Localization
Membrane; Single-pass type I membrane protein. Cell junction.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
PECAM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)
Application Notes
This CD31/PECAM1 antibody (clone MEC 7.46) is useful for Immunoprecipitation, Immunohistochemistry on frozen sections, Flow Cytometry and Immunofluorescence.
Publications
Read Publications using
NB100-1642 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD31/PECAM-1 Antibody (MEC 7.46)

  • adhesion molecule
  • anti-CD31
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • mec 7.46 cd31
  • mec 7.46 clone
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31/PECAM1 is commonly used as an endothelial marker, particularly in angiogenesis. CD31/PECAM1 is important in assisting placental and endometrial vascular development. However, CD31/PECAM1 is also associated with many negative angiogenic occurrences, particularly those within or related to tumors and other disease lesions. In MS, plasma microparticules carry CD31/PECAM1 and are important in the formation of brain lesions associated with MS. In cardiac angiosarcoma, CD31/PECAM1 presence is indicative of the severity of the disease. In prostate tumor cells, CD31/PECAM1 is upregulated and associated with increased levels of HIF1a, VEGF, and other hypoxic markers. This relationship seems to indicate that when prostate cancer reaches a sufficient state of development that intense angiogenesis must occur in order to support the ongoing growth and survival of the cancer cells. In all, CD31/PECAM1 is a great marker for normal and disease-induced angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NB100-1642)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo.


Filter By Application
FLOW
(2)
ICC/IF
(3)
IHC
(3)
IHC-Fr
(3)
IHC-P
(1)
In vivo
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-1642 Applications Species
Liang H, Deng L, Hou Y et al. Host STING-dependent MDSC mobilization drives extrinsic radiation resistance Nat Commun. 2017 Nov 23 [PMID: 29170400] (IHC-Fr, Mouse) IHC-Fr Mouse
Ielacqua GD, Schlegel F, Fuchtemeier M et al. Magnetic Resonance Q Mapping Reveals a Decrease in Microvessel Density in the arcAbeta Mouse Model of Cerebral Amyloidosis. Front Aging Neurosci 2015 [PMID: 26834622] (IHC, Mouse) IHC Mouse
Yang P, Hong MS, Fu C et al. Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions. Surgery 2016 Feb [PMID: 26387788]
Haxho F, Allison S, Alghamdi F et al. Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinoma. Breast Cancer Dove Med Press) 2014 Dec 19 [PMID: 25525387] (IHC-P, Human)

Details:
Using the DyLight 488 conjugated version of NB100-1642, catalog number NB100-1642G.
IHC-P Human
Burgi S, Seuwen A, Keist R et al. In Vivo Imaging of Hypoxia-Inducible Factor Regulation in a Subcutaneous and Orthotopic GL261 Glioma Tumor Model Using a Reporter Gene Assay. Mol Imaging. 2014 Sep 01 [PMID: 25248521]
Kalet BT, Anglin SR, Handschy A et al. Transcription Factor Ets1 Cooperates with Estrogen Receptor alpha to Stimulate Estradiol-Dependent Growth in Breast Cancer Cells and Tumors. PLoS One 2013 Jul 9 [PMID: 23874775] (ICC/IF, Mouse) ICC/IF Mouse
Lampugnani MG, Zanetti A, Breviario F, Balconi G, Orsenigo F, Corada M, Spagnuolo R, Betson M, Braga V, Dejana E. VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam. Mol Biol Cell;13(4):1175-89. 2002 Apr. [PMID: 11950930] (ICC/IF, Mouse) ICC/IF Mouse
Zips D, Eicheler W, Bruchner K, Jackisch T, Geyer P, Petersen C, van der Kogel AJ, Baumann M. Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouse. Int J Radiat Biol;77(12):1185-93. 2001 Dec. [PMID: 11747543]
Leenders W, van Altena M, Lubsen N, Ruiter D, De Waal R. In vivo activities of mutants of vascular endothelial growth factor (VEGF) with differential in vitro activities. Int J Cancer;91(3):327-33. 2001 Feb 1. [PMID: 11169955] (IHC-Fr, Mouse) IHC-Fr Mouse
Balconi G, Spagnuolo R, Dejana E. Development of endothelial cell lines from embryonic stem cells: A tool for studying genetically manipulated endothelial cells in vitro. Arterioscler Thromb Vasc Biol;20(6):1443-51. 2000 Jun. [PMID: 10845856] (FLOW, Mouse) FLOW Mouse
Show All 18 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NB100-1642) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1642AF405
Alexa Fluor 488 Labeled NB100-1642AF488
Alexa Fluor 647 Labeled NB100-1642AF647
Alexa Fluor 700 Labeled NB100-1642AF700
Allophycocyanin Labeled NB100-1642APC
Biotin Labeled NB100-1642B
DyLight 350 Labeled NB100-1642UV
DyLight 405 Labeled NB100-1642V
DyLight 488 Labeled NB100-1642G
DyLight 550 Labeled NB100-1642R
DyLight 650 Labeled NB100-1642C
DyLight 680 Labeled NB100-1642FR
DyLight 755 Labeled NB100-1642IR
FITC Labeled NB100-1642F
HRP Labeled NB100-1642H
PE Labeled NB100-1642PE
PerCP Labeled NB100-1642PCP

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Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NB100-1642). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD31/PECAM-1 Antibody (NB100-1642)

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Research Areas for CD31/PECAM-1 Antibody (NB100-1642)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot