CD31/PECAM-1 Antibody (LCI-4) - BSA Free

Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.

• Reactivity: Po
• Applications: Flow, IHC, IP
• Clone: LCI-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD31/PECAM-1 Antibody (LCI-4) - BSA Free Summary

• Description: Novus Biologicals Mouse CD31/PECAM-1 Antibody (LCI-4) - BSA Free (NB100-65336) is a monoclonal antibody validated for use in IHC, Flow and IP. Anti-CD31/PECAM-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Porcine CD31/human IgGFc fusion protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PECAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Clone LCI4 has been shown to stimulate tyrosine phosphorylation of CD31 on endothelial cells. For Flow Cytometry use 10ul of the suggested working dilution to label 10^6 cells or 100ul whole blood. This antibody does not work in Western Blotting.
• Theoretical MW: 82.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivities: Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CD31/PECAM-1 Antibody (LCI-4) - BSA Free

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (NB100-65336)(2)

Publications using NB100-65336

• Chen Y, Gao P, Huang L et al. A tough nitric oxide-eluting hydrogel coating suppresses neointimal hyperplasia on vascular stent Nature communications 2021-12-06 [PMID: 34873173] (Immunohistochemistry, Porcine)
• Tefft BJ, Uthamaraj S, Harbuzariu A, Harburn J. Nanoparticle mediated cell capture enables rapid endothelialization of a novel bare metal stent. Tissue Eng Part A. 2018-02-12 [PMID: 29431053] (IF/IHC, Porcine)

FAQs for CD31/PECAM-1 Antibody (NB100-65336). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for an antibody to stain for endothelial cells in whole mount porcine arteries. These arteries have been fixed in formalin and then cut open for staining. I'm planning on doing fluorescent confocal microscopy to visualize staining. Please let me know which Ab you'd recommend. The protein target would be CD31/PECAM and smooth muscle actin. I'm looking for advice on the best antibody against CD31 to use in porcine arteries for confocal immunofluorescence in whole tissue.
   • NB100-65336 is our only antibody validated for use in ICC on porcine samples. This will be the best choice for your application.

Bioinformatics

• Gene Symbol: PECAM1
• Entrez:
  • 5175(Human)
  • 396941(Porcine)
• Uniprot:
  • Q95242()
  • Q95242()