CD31/PECAM-1 Antibody (C31.7)


Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using Azide/BSA FREE version of NBP2-15188. Formalin-paraffin human Angiosarcoma stained with CD31 MAb (C31.7).
Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using the Azide and BSA Free version of NBP2-15188. Detection of CD31 in THP-1 lysate using CD31 MAb (C31.7)
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Analysis using the Azide and BSA Free version of NBP2-15188. Staining of human Tonsil stained with CD31 MAb (C31.7).
Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Human CD31 on Jurkat Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31 monoclonal antibody (C31.7).
Flow Cytometry: CD31/PECAM-1 Antibody (C31.7) [NBP2-15188] - Human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31 monoclonal antibody (C31.7).

Product Details

Reactivity Hu, Cm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
0.2 mg/ml

CD31/PECAM-1 Antibody (C31.7) Summary

Human recombinant CD31 protein
Cell surface and cytoplasmic
Endothelial Cell Marker
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Prepared in 10mM PBS with 0.05% BSA.
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified


  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location: 17q23.3
Mol. Weight of Antigen: ~100kDa (endothelium) and ~130kDa (platelets)
Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Immunocytochemistry/Immunofluorescence application cited in literature.

Reactivity Notes

Human, Cynomolgus Monkey and Rabbit. Weakly reacts with Rat. Feedback has been negative on pig kidney paraffin sections.

Alternate Names for CD31/PECAM-1 Antibody (C31.7)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule


CD31 (PECAM-1, or platelet endothelial cell adhesion molecule-1) is a surface protein expressed by endothelial cells, monocytes, platelets, granulocytes, and lymphocyte subsets, and makes up a large portion of endothelial intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Reports indicate that CD31 interacts with CD38 and is involved in cellular interactions resulting in wound healing and angiogenesis. Expression of CD31 on CD4+ T lymphocytes, helps to control T lymphocyte activation, because in the absence of CD31, T cells have a greater propensity to become activated, resulting in increased susceptibility to become apoptotic. This impact of CD31 loss becomes most pronounced during severe, inflammatory, and immunological stresses such as those caused by systemic Salmonella infection. This identifies a novel role for CD31 in regulating CD4 T homeostasis. This monoclonal antibody recognizes a 100kDa glycoprotein in endothelial cells and 130kDa in platelets. This antibody reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. Antibody to CD31 is of value in the study of benign and malignant vascular tumors. Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NBP2-15188). (Showing 1 - 1 of 1 FAQ).

  1. We bought this product unit size is 20ug, concentration is 0.2mg/mL. Is the total volume 20uL or is the total volume of 100uL of solution giving 20ug total of pure antibody?
    • This product should be supplied in 100ul of buffer with a total antibody amount of 20ug of mouse IgG1 Kappa protein.

Secondary Antibodies


Isotype Controls

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Contact Information

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Gene Symbol PECAM1

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