Raw 264.7 Whole Cell Lysate


Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30157] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2) [NBP1-30157].

Product Details

Reactivity MuSpecies Glossary
Applications WB
1.0 mg/ml

Order Details

Raw 264.7 Whole Cell Lysate Summary


Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
1.0 mg/ml


  • Western Blot
Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Lysate Details for Raw 264.7

Blood Lymph
Protein State
Subcellular Fraction


NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Large protein samples should be heated to 65C for 5-10 minutes prior to loading.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Additional Raw 264.7 Whole Cell Lysate Products

Raw 264.7 NBP1-30157

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