Raw 264.7 Whole Cell Lysate Summary
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
Lysate Details for Raw 264.7
NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Large protein samples should be heated to 65C for 5-10 minutes prior to loading.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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