Caveolin-1 Antibody (7C8)

Images

 
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or ...read more
Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
7C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Caveolin-1 Antibody (7C8) Summary

Background
Caveolin 1 and 2 are usually both expressed and assembled into hetero-oligomers in the endoplasmic reticulum and Golgi apparatus. Caveolin 1 also interacts with lipids and lipid anchors, and may therefore function in both membrane traffic and lipid traffic. In vitro assays confirm that Caveolin 1 interacts with cholesterol, suggesting that Caveolin 1 may organize membrane cholesterol in cave...olae. Caveolin 1 has high affinity to long chain unsaturated fatty acids. Additionally, Caveolin 1 may play a role in the export and import of cellular cholesterol by caveolae.
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Immunogen
Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes
Localization
Cell Membrane
Marker
Caveolae Marker, Endosome Marker
Specificity
This is specific for caveolin alpha and beta proteins.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CAV1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug per million cells
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Immunoprecipitation 1-2 ug / 500 ug of protein
  • Western Blot 1:1000-1:4000
Application Notes
In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24758774). Use in immunoblotting reported in scientific literature (PMID: 31759628).
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caveolin-1 Overexpression Lysate
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-615 in the following applications:

Publications
Read Publications using
NB100-615 in the following applications:

  • 1 publication
  • 1 publication
  • IB
    1 publication
  • 11 publications
  • IHC
    2 publications
  • 1 publication
  • 2 publications
  • IP
    2 publications
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Caveolin-1 Antibody (7C8)

  • CAV
  • CAV1
  • caveolae protein, 22kD
  • caveolin 1, caveolae protein, 22kDa
  • Caveolin1
  • Caveolin-1
  • cell growth-inhibiting protein 32
  • MSTP085
  • VIP21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caveolin-1 Antibody (NB100-615)(33)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Drosophila melanogaster, ICC/IF, Sheep.

We have publications tested in 9 applications: Flow, Human, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
Flow
(1)
Human
(1)
IB
(1)
ICC/IF
(11)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(12)
Mouse
(9)
Rat
(2)
Drosophila melanogaster
(1)
ICC/IF
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-615 Applications Species
Wang J, Xu J, Zang G et al. trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis Research (Washington, D.C.) Apr 4 2022 [PMID: 35465346] (WB, Human) WB Human
Sanchez-Solana B, Wang D, Qian X Et al. The tumor suppressor activity of DLC1 requires the interaction of its START domain with Phosphatidylserine, PLCD1, and Caveolin-1 Molecular cancer Nov 2 2021 [PMID: 34727930]
Collins DP, Osborn MJ, Steer CJ. et al. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins Cytotherapy Aug 1 2021 [PMID: 34551876] (Human, ICC/IF)

Details:
Citation using the Azide and BSA Free format of this antibody.
Human ICC/IF
Kurebayashi Y, Bajimaya S, Watanabe M et al. Human parainfluenza virus type 1 regulates cholesterol biosynthesis and establishes quiescent infection in human airway cells PLOS Pathogens Sep 16 2021 [PMID: 34529742] (WB, Human) WB Human
Anderson-Baucum E, PiNeros AR, Kulkarni A et al. Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype Cell metabolism Sep 7 2021 [PMID: 34496231] (IHC-P, Mouse) IHC-P Mouse
Daneva Z, Ottolini M, Chen YL et al. Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice eLife Sep 7 2021 [PMID: 34490843]
Jiang H, Wu T, Liu J, et al. Caveolae/rafts protect human cerebral microvascular endothelial cells from Streptococcus suis serotype 2 alpha -enolase-mediated injury Veterinary microbiology Jan 6 2021 [PMID: 33445055] (ICC/IF, Human) ICC/IF Human
Srisomboon Y, Squillace DL, Maniak PJ et al. Fungal allergen-induced IL-33 secretion involves cholesterol-dependent, VDAC-1-mediated ATP release from the airway epithelium J. Physiol. (Lond.) Feb 27 2020 [PMID: 32103508] (ICC/IF, Human) ICC/IF Human
Savio, L E B, de Andrade Mello, P Et al. P2X7 receptor activation increases expression of caveolin-1 and formation of macrophage lipid rafts, thereby boosting CD39 activity. J Cell Sci Mar 6 2020 [PMID: 32005701] (IHC, Drosophila melanogaster) IHC Drosophila melanogaster
Kim SA, Kim SJ, Choi YA et al. Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP Biochem. Biophys. Res. Commun. Nov 20 2019 [PMID: 31759628] (IB, Human, Mouse) IB Human, Mouse
Show All 33 Publications.

Reviews for Caveolin-1 Antibody (NB100-615) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-615:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Caveolin-1 NB100-615
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Human 03/05/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdipose tissue
SpeciesHuman
Lotb

Comments

CommentsAntibody dilution 1:250
Immunocytochemistry Caveolin-1 NB100-615
Enlarge
5
reviewed by:
Lakshmi Reddy Bollu
ICC Human 08/12/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cancer cell line
SpeciesHuman
Lotb
  5
reviewed by:
cynthia morimoto
IF Human 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 24348251
SpeciesHuman

Product General Protocols

View specific protocols for Caveolin-1 Antibody (NB100-615): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caveolin-1 Antibody (NB100-615). (Showing 1 - 1 of 1 FAQs).

  1. What is the sodium azide percentage for this product?
    • 0.05% sodium azide 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caveolin-1 Antibody (NB100-615)

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
03/05/2021
Application: IHC-Fr
Species: Human

Lakshmi Reddy Bollu
08/12/2015
Application: ICC
Species: Human

 
cynthia morimoto
12/19/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CAV1