Caveolin-1 Antibody (7C8)

Images

 
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
7C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Caveolin-1 Antibody (7C8) Summary

Background
Caveolin 1 and 2 are usually both expressed and assembled into hetero-oligomers in the endoplasmic reticulum and Golgi apparatus. Caveolin 1 also interacts with lipids and lipid anchors, and may therefore function in both membrane traffic and lipid traffic. In vitro assays confirm that Caveolin 1 interacts with cholesterol, suggesting that Caveolin 1 may organize membrane cholesterol in cave...olae. Caveolin 1 has high affinity to long chain unsaturated fatty acids. Additionally, Caveolin 1 may play a role in the export and import of cellular cholesterol by caveolae.
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Immunogen
Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes
Localization
Cell Membrane
Marker
Caveolae Marker, Endosome Marker
Specificity
This is specific for caveolin alpha and beta proteins.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CAV1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • Flow Cytometry 1 ug per million cells
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Immunoprecipitation 1-2 ug / 500 ug of protein
Application Notes
In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24758774). Use in immunoblotting reported in scientific literature (PMID: 31759628).
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caveolin-1 Overexpression Lysate
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
Caveolin-1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-615 in the following applications:

Publications
Read Publications using
NB100-615 in the following applications:

  • 1 publication
  • IB
    1 publication
  • 9 publications
  • IHC
    1 publication
  • 1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Caveolin-1 Antibody (7C8)

  • CAV
  • CAV1
  • caveolae protein, 22kD
  • caveolin 1, caveolae protein, 22kDa
  • Caveolin1
  • Caveolin-1
  • cell growth-inhibiting protein 32
  • MSTP085
  • VIP21

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caveolin-1 Antibody (NB100-615)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Sheep.

We have publications tested in 8 applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
Flow
(1)
IB
(1)
ICC/IF
(9)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
IP
(2)
WB
(7)
All Applications
Filter By Species
Human
(8)
Mouse
(8)
Rat
(2)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-615 Applications Species
Kim SA, Kim SJ, Choi YA et al. Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP Biochem. Biophys. Res. Commun. Nov 20 2019 [PMID: 31759628] (IB, Human, Mouse) IB Human, Mouse
de Andrade Mello P, Bian S, Savio LEB et al. Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death Oncotarget. 2017 Sep 14 [PMID: 28978031] (IP, WB, Mouse) IP, WB Mouse
Liu C, Liu T, Yu X et al. A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Acta Pharm Sin B. 2017 Apr 1 [PMID: 28540174] (Flow, ICC/IF, Human)

Details:
Citation used the DyLight 488 format of this antibody.
Flow, ICC/IF Human
Arima Mitsuru, Cui Dan, Kimura Tokuhiro et al. Basigin can be a therapeutic target to restore the retinal vascular barrier function in the mouse model of diabetic retinopathy. Scientific Reports 2016 [PMID: 27917946] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Ayaz D, Diniz G, Kahraman DS et al. The evaluation of the caveolin-1 and AT-rich interactive domain 1 alpha expressions in uterine smooth muscle tumors. Indian J Pathol Microbiol. Aug 11 2016 [PMID: 27510664] (IHC) IHC
Li Z, Scott MJ, Fan EK et al. Tissue damage negatively regulates LPS-induced macrophage necroptosis Cell Death Differ. 2016 Sep 1 [PMID: 26943325] (WB, ICC/IF, Mouse)

Details:
Citation used the Alexa Fluor 488 format of this antibody.
WB, ICC/IF Mouse
Francesca G, Cinzia P, Antonella B et al. SMN affects membrane remodelling and anchoring of the protein synthesis machinery. J. Cell. Sci. 2016 Jan 07 [PMID: 26743087] (ICC/IF, Human) ICC/IF Human
Boyce AK, Kim MS, Wicki-Stordeur LE, Swayne LA et al. ATP stimulates Pannexin 1 internalisation to endosomal compartments Biochem. J. 2015 Jul 20 [PMID: 26195825] (ICC/IF, Mouse) ICC/IF Mouse
Yokomori H, Ando W, Yoshimura K et al. Increases in endothelial caveolin-1 and cavins correlate with cirrhosis progression. Micron 2015 Sep [PMID: 26086560] (Human) Human
Kallenborn-Gerhardt W, Lu R, Bothe A et al. Phosphodiesterase 2A Localized in the Spinal Cord Contributes to Inflammatory Pain Processing. Anesthesiology 2014 Apr 22 [PMID: 24758774] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 24 Publications.

Reviews for Caveolin-1 Antibody (NB100-615) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-615:
Filter by Applications
ICC
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Caveolin-1 NB100-615
Enlarge
reviewed by:
Anonymous
ICC Human 08/12/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cancer cell line
SpeciesHuman
Lotb

Blocking

Blocking Details2.5% normal goat serum

Primary Anitbody

Dilution Ratio1:200 in 2.5% goat serum

Secondary Antibody

Secondary DescriptionGoat anti-mouse alexa fluor 594

Details

Detection NotesOlympus confocal microscope imaging
Fixation Details4% PFA for fixation for 10 minutes, 0.1% triton x100 in PBS for 15 minutes at room temperature
Wash DescriptionThree washes with PBST, each for 10 minutes.
 
reviewed by:
Anonymous
IF Human 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 24348251
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 24348251

Details

Detection NotesSee PMID: 24348251

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

View specific protocols for Caveolin-1 Antibody (NB100-615): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caveolin-1 Antibody (NB100-615). (Showing 1 - 1 of 1 FAQs).

  1. What is the sodium azide percentage for this product?
    • 0.05% sodium azide 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-615AF350
Alexa Fluor 405 NB100-615AF405
Alexa Fluor 488 NB100-615AF488
Alexa Fluor 532 NB100-615AF532
Alexa Fluor 594 NB100-615AF594
Alexa Fluor 647 NB100-615AF647
Alexa Fluor 700 NB100-615AF700
Alexa Fluor 750 NB100-615AF750
Allophycocyanin NB100-615APC
Biotin NB100-615B
DyLight 350 NB100-615UV
DyLight 405 NB100-615V
DyLight 488 NB100-615G
DyLight 550 NB100-615R
DyLight 650 NB100-615C
DyLight 680 NB100-615FR
DyLight 755 NB100-615IR
FITC NB100-615F
HRP NB100-615H
Janelia Fluor 549 NB100-615JF549
Janelia Fluor 646 NB100-615JF646
PE NB100-615PE
PerCP NB100-615PCP

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
08/12/2015
Application: ICC
Species: Human

 
Anonymous
12/19/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CAV1
COVID-19 update