Caveolin-1 Antibody (7C8)

Images

 
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or ...read more
Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo ...read more
Immunocytochemistry/ Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An ...read more
Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Impairment of RPA foci formation and the DNA damage checkpoint in CBP and p300 depleted cells.(A) Effects of CBP and p300 depletion on DSB-induced RPA and CHK1 activation. H1299 cells were pre-transfected with ...read more
Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene and poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, WB
Clone
7C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Caveolin-1 Antibody (7C8) Summary

Background
Caveolin 1 and 2 are usually both expressed and assembled into hetero-oligomers in the endoplasmic reticulum and Golgi apparatus. Caveolin 1 also interacts with lipids and lipid anchors, and may therefore function in both membrane traffic and lipid traffic. In vitro assays confirm that Caveolin 1 interacts with cholesterol, suggesting that Caveolin 1 may organize membrane cholesterol in cave...olae. Caveolin 1 has high affinity to long chain unsaturated fatty acids. Additionally, Caveolin 1 may play a role in the export and import of cellular cholesterol by caveolae.
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Immunogen
Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes
Localization
Cell Membrane
Marker
Caveolae Marker, Endosome Marker
Specificity
This is specific for caveolin alpha and beta proteins.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CAV1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug per million cells
  • Immunoblotting reported in scientific literature (PMID 31759628)
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24758774)
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Immunoprecipitation 1-2 ug / 500 ug of protein
  • Western Blot 1:1000-1:4000
Application Notes
In Western blot, a band is observed ~23 kDa, representing the Caveolin 1 protein. A band at ~21 kDa may also be observed depending on the lysates used.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caveolin-1 Overexpression Lysate
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-615 in the following applications:

Publications
Read Publications using
NB100-615 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Caveolin-1 Antibody (7C8)

  • CAV
  • CAV1
  • caveolae protein, 22kD
  • caveolin 1, caveolae protein, 22kDa
  • Caveolin1
  • Caveolin-1
  • cell growth-inhibiting protein 32
  • MSTP085
  • VIP21

Background

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caveolin-1 Antibody (NB100-615)(37)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila melanogaster, Sheep.

We have publications tested in 9 applications: FLOW, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
FLOW
(1)
IB
(1)
ICC/IF
(13)
IF/IHC
(3)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(14)
Mouse
(10)
Rat
(3)
Drosophila melanogaster
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB100-615 Applications Species
Zhu A, Lin Y, Hu X et al. Treadmill exercise decreases cerebral edema in rats with local cerebral infarction by modulating AQP4 polar expression through the caveolin-1/TRPV4 signaling pathway Brain research bulletin 2022-10-01 [PMID: 35961528] (IHC-Fr, Rat) IHC-Fr Rat
Yokomori H, Inagaki Y, Ando W, Hara M. Spatiotemporal expression of matrix metalloproteinase-1 in progression of nonalcoholic steatohepatitis. Journal of Modern Human Pathology. 2016-03-30 (IHC-P, Human) IHC-P Human
Margaret JV Deciphering the Role of Caveolae in Human Meniscus Fibrochondrocytes Thesis 2022-01-01 (ICC/IF, Human) ICC/IF Human
Dalton CM, Schlegel C, Hunter CJ Caveolin-1: A Review of Intracellular Functions, Tissue-Specific Roles, and Epithelial Tight Junction Regulation Biology 2023-11-05 [PMID: 37998001] (IHC) IHC
Wang S, Ichinomiya T, Savchenko P Et al. Subpial delivery of adeno-associated virus 9-synapsin-caveolin-1 (AAV9-SynCav1) preserves motor neuron and neuromuscular junction morphology, motor function, delays disease onset, and extends survival in hSOD1(G93A) mice Theranostics 2022-08-01 [PMID: 35910808] (IF/IHC, Mouse)

Details:
Citation using the Alexa Fluor 594 version of this antibody.
IF/IHC Mouse
Wang J, Xu J, Zang G et al. trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis Research (Washington, D.C.) 2022-04-04 [PMID: 35465346] (WB, Human) WB Human
Sanchez-Solana B, Wang D, Qian X Et al. The tumor suppressor activity of DLC1 requires the interaction of its START domain with Phosphatidylserine, PLCD1, and Caveolin-1 Molecular cancer 2021-11-02 [PMID: 34727930]
Collins DP, Osborn MJ, Steer CJ. et al. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins Cytotherapy 2021-08-01 [PMID: 34551876] (ICC/IF, Human) ICC/IF Human
Kurebayashi Y, Bajimaya S, Watanabe M et al. Human parainfluenza virus type 1 regulates cholesterol biosynthesis and establishes quiescent infection in human airway cells PLOS Pathogens 2021-09-16 [PMID: 34529742] (WB, Human) WB Human
Anderson-Baucum E, PiNeros AR, Kulkarni A et al. Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype Cell metabolism 2021-09-07 [PMID: 34496231] (IHC-P, Mouse) IHC-P Mouse
Show All 37 Publications.

Reviews for Caveolin-1 Antibody (NB100-615) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-615:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Caveolin-1 NB100-615
Enlarge
5
reviewed by:
auriane ernault
IHC-Fr Human 03/05/2021
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdipose tissue
SpeciesHuman
Lotb

Comments

CommentsAntibody dilution 1:250
Immunocytochemistry Caveolin-1 NB100-615
Enlarge
5
reviewed by:
Verified Customer
ICC Human 08/12/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cancer cell line
SpeciesHuman
Lotb
  5
reviewed by:
Verified Customer
IF Human 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID: 24348251
SpeciesHuman

Product General Protocols

View specific protocols for Caveolin-1 Antibody (NB100-615): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caveolin-1 Antibody (NB100-615). (Showing 1 - 1 of 1 FAQs).

  1. What is the sodium azide percentage for this product?
    • 0.05% sodium azide 
mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

auriane ernault
03/05/2021
Application: IHC-Fr
Species: Human

Verified Customer
08/12/2015
Application: ICC
Species: Human

 
Verified Customer
12/19/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CAV1