Caveolin-2 Antibody

Images

 
Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116] - Paraffin-embedded FaDu xenograft, using antibody at 1:500 dilution.
Western Blot: Caveolin-2 Antibody [NBP1-31116] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Fe, Pm, Rb, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.94 mg/ml

Order Details

Caveolin-2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 2. The exact sequence is proprietary.
Localization
Golgi apparatus membrane, Single-pass membrane protein, Cell membrane, Single-pass membrane protein, Membrane, caveola, Single-pass membrane protein, Golgi apparatus membrane, Cell membrane, Membrane, caveola
Marker
Caveolae Marker
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Sheep (100%), Rhesus Macaque (100%), Bovine (100%), Feline (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAV2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31116 in the following applications:

Reactivity Notes

Chimpanzee (100%), Cat (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caveolin-2 Antibody

  • CAV
  • CAV2
  • caveolae protein, 20-kD
  • caveolin 2 isoform a and b
  • caveolin 2
  • Caveolin2
  • Caveolin-2
  • MGC12294

Background

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins (1). Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Caveolins 1 and 2 are similar in most respects, however, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell (2). Caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

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Review for Caveolin-2 Antibody (NBP1-31116) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-31116:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Caveolin-2 NBP1-31116
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reviewed by:
Anonymous
ICC Pig 11/28/2016
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Summary

ApplicationImmunocytochemistry
Sample Testedabecular meshwork cells
SpeciesPig

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
11/28/2016
Application: ICC
Species: Pig

Bioinformatics

Gene Symbol CAV2
Entrez