EphB2 Antibody [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody ...read more
D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #AB-108-C, ...read more
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room ...read more
EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at ...read more
EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) ...read more
EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at ...read more
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

EphB2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763
Specificity
Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 5 Reviews rated 4.2
using
AF467 in the following applications:

Publications
Read Publications using
AF467 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB2 Antibody [Unconjugated]

  • CAPB
  • Cek5
  • Drt
  • DRTEphB2
  • EC 2.7.10
  • EC 2.7.10.1
  • EK5
  • elk-related tyrosine kinase
  • EPH receptor B2
  • eph tyrosine kinase 3
  • EphB2
  • EPH-like kinase 5
  • ephrin type-B receptor 2
  • EPHT3MGC87492
  • EPTH3
  • Erk
  • ERKHek5
  • Hek5
  • Nuk
  • PCBC
  • protein-tyrosine kinase HEK5
  • Qek2
  • Renal carcinoma antigen NY-REN-47
  • Sek3
  • Tyro5
  • Tyrosine-protein kinase receptor EPH-3
  • Tyrosine-protein kinase TYRO5

Background

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF

Publications for EphB2 Antibody (AF467)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 11 applications: Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Neutralization, Simple Western, Western Blot.


Filter By Application
Functional Assay
(1)
ICC
(9)
IF
(1)
IHC
(10)
IHC-Fr
(4)
IHC-P
(6)
Immunoprecipitation
(9)
In Vivo
(1)
Neutralization
(2)
Simple Western
(1)
Western Blot
(18)
All Applications
Filter By Species
Human
(15)
Mouse
(24)
Rat
(2)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using AF467 Applications Species
Gonz�lez-Gonz�lez, IM;Gray, JA;Ferreira, J;Conde-Dusman, MJ;Bouchet, D;Perez-Ota�o, I;Groc, L; GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development Cell reports 2023-05-06 [PMID: 37149869] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Y Suzuki, K Okabayashi, H Hasegawa, M Tsuruta, R Seishima, T Tokuda, Y Kitagawa Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancer Oncology Letters, 2022-07-19;24(3):316. 2022-07-19 [PMID: 35949596] (IHC, Human) IHC Human
SL Banerjee, F Lessard, FJM Chartier, K Jacquet, AI Osornio-He, V Teyssier, K Ghani, N Lavoie, JN Lavoie, M Caruso, P Laprise, S Elowe, JP Lambert, N Bisson EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks Cell Reports, 2022-07-05;40(1):111031. 2022-07-05 [PMID: 35793621] (Western Blot, Human) Western Blot Human
AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse) IF Mouse
E López-Arri, B Yan, T Lobo-Jarne, Y Guillén, S Menéndez, M Andreu, A Bigas, M Iglesias, L Espinosa Accumulation of Paneth Cells in Early Colorectal Adenomas Is Associated with Beta-Catenin Signaling and Poor Patient Prognosis Cells, 2021-10-28;10(11):. 2021-10-28 [PMID: 34831152] (IHC, Human) IHC Human
L M Gagné, N Morin, N Lavoie, N Bisson, JP Lambert, FA Mallette, MÉ Huot Tyrosine phosphorylation of DEPTOR functions as a molecular switch to activate mTOR signaling The Journal of Biological Chemistry, 2021-10-09;297(5):101291. 2021-10-09 [PMID: 34634301] (Western Blot, Human) Western Blot Human
MS Ahmed, P Wang, NUN Nguyen, Y Nakada, I Menendez-M, M Ismail, R Bachoo, M Henkemeyer, HA Sadek, ES Kandil Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain Proceedings of the National Academy of Sciences of the United States of America, 2021-03-09;118(10):. 2021-03-09 [PMID: 33627480] (Western Blot, Mouse) Western Blot Mouse
A Kischel, C Audouard, MA Fawal, A Davy Ephrin-B2 paces neuronal production in the developing neocortex BMC Dev. Biol., 2020-05-13;20(1):12. 2020-05-13 [PMID: 32404061] (In Vivo, Mouse) In Vivo Mouse
ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 2020-05-04 [PMID: 32366833] (Western Blot, Human) Western Blot Human
D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands J. Leukoc. Biol., 2020-04-26;0(0):. 2020-04-26 [PMID: 32337793] (IHC, Human) IHC Human
Show All 42 Publications.

Reviews for EphB2 Antibody (AF467) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Mouse, Other.

Reviews using AF467:
Filter by Applications
WB
(3)
IP
(1)
Simple Western
(1)
All Applications
Filter by Species
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot EphB2 AF467
Enlarge
4
reviewed by:
STAVROULA THEOFANOUS
WB Mouse 07/05/2018
View

Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord
SpeciesMouse
LotCFK071804A

Comments

CommentsBlot Loading
• ANTIBODY: Human/Mouse EphB2Antibody cat noAF542
Lane1: Precision Plus Protein™ Dual Color Standards7ul
Lane2:Ephb1 receptor (SC tissue from naive C57BL6j mouse)
Western blot experiment details
Gel:4-20% SDS page
Tranfer apparatus: Trans-Blot® Turbo™ Transfer System
Membrane: midi size nitrocellulose
Time: 25min at 75-125V
Primary ab:Goat –rat EphB1 Ab 1mg for 20h
Secondary ab: rabbit a-goat 1:5,000 for 1h
Substrate: HRP for 15min
Immunoprecipitation EphB2 AF467
Enlarge
4
reviewed by:
STAVROULA THEOFANOUS
IP Mouse 04/16/2018
View

Summary

ApplicationImmunoprecipitation
Sample Testedbrain and spinal cord
SpeciesMouse
LotCVT0311121

Comments

CommentsBlot Loading
• ANTIBODY: Human/Mouse EphB2Antibody cat noAF467
Lane1: PreĐision Plus Protein™ Dual Color Standards8ul
Lane2:Ephb2 receptors IP 1mg protein (SC tissue)
Lane3:Ephb2 receptors no IP 25ug (SC tissue)
Lane4:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue)
Lane5:Ephb2 receptors IP 1mg (SC tissue)
Lane6:Ephb2 receptors no IP 25ug (SC tissue)
Lane7:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue)
Western blot experiment details
Gel:4-20% SDS page
Tranfer apparatus: Trans-Blot® Turďo™ Transfer System
Membrane: midi size nitrocellulose
Time: 25min at 75-125V
Primary ab:Goat –m/h EphB2 Ab 1mg for 20h
Secondary ab: donkey a-goat 1:15,000 for 1h
Substrate: HRP for 15min
Simple Western EphB2 AF467
Enlarge
5
reviewed by:
Verified Customer
Simple Western Mouse 07/12/2017
View

Summary

ApplicationSimple Western
Sample Testedmouse brain lysate
SpeciesMouse

Comments

CommentsSimple Western: anti-EphB2 antibody [biotechne AF467]
Simple Western lane view shows a specific 128 kDa signal for EphB2 in 0.5 mg/ml of mouse brain lysate (lysis buffer from ARY014 proteome profiler kit). This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50).
  4
reviewed by:
Verified Customer
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21276420
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Other 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22144690
SpeciesOther

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Blogs on EphB2.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

STAVROULA THEOFANOUS
07/05/2018
Application: WB
Species: Mouse

STAVROULA THEOFANOUS
04/16/2018
Application: IP
Species: Mouse

Verified Customer
07/12/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol EPHB2
Uniprot