EphB2 Antibody [Unconjugated]

Images

 
COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody ...read more
EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) ...read more
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at ...read more
EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at ...read more
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room ...read more
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at ...read more
D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #AB-108-C, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

EphB2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763
Specificity
Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
using
AF467 in the following applications:

Publications
Read Publications using
AF467 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB2 Antibody [Unconjugated]

  • CAPB
  • Cek5
  • Drt
  • DRTEphB2
  • EC 2.7.10
  • EC 2.7.10.1
  • EK5
  • elk-related tyrosine kinase
  • EPH receptor B2
  • eph tyrosine kinase 3
  • EphB2
  • EPH-like kinase 5
  • ephrin type-B receptor 2
  • EPHT3MGC87492
  • EPTH3
  • Erk
  • ERKHek5
  • Hek5
  • Nuk
  • PCBC
  • protein-tyrosine kinase HEK5
  • Qek2
  • Renal carcinoma antigen NY-REN-47
  • Sek3
  • Tyro5
  • Tyrosine-protein kinase receptor EPH-3
  • Tyrosine-protein kinase TYRO5

Background

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB2 Antibody (AF467)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 10 applications: ICC, ICC Fluorescence, IHC, IHC - Paraffin embedded, IHC - paraffin, IHC Frozen, IHC Paraffin-embedded, IP, In vivo, WB.


Filter By Application
ICC
(1)
ICC Fluorescence
(1)
IHC
(3)
IHC - Paraffin embedded
(1)
IHC - paraffin
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
IP
(5)
In vivo
(1)
WB
(7)
All Applications
Filter By Species
Human
(7)
Mouse
(13)
Rat
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF467 Applications Species
S Miura, A Suzuki Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28943029] (IHC,, Mouse) IHC Mouse
YH Liu, JW Tsai, JL Chen, WS Yang, PC Chang, PL Cheng, DL Turner, Y Yanagawa, TW Wang, JY Yu Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon Sci Rep, 2017;7(0):42895. 2017 [PMID: 28276447] (ICC,, Mouse) ICC Mouse
N Tüysüz, L van Bloois, S van den Br, H Begthel, MM Verstegen, LJ Cruz, L Hui, LJ van der La, J de Jonge, R Vries, E Braakman, E Mastrobatt, JJ Cornelisse, H Clevers, D Ten Berge Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells Nat Commun, 2017;8(0):14578. 2017 [PMID: 28262686] (IHC,, Human) IHC Human
Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells Oncogene, 2016;0(0):. 2016 [PMID: 27819675] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model Mol Psychiatry, 2016;0(0):. 2016 [PMID: 27646263] (IHC Frozen,, Mouse) IHC Frozen Mouse
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016;7(0):12329. 2016 [PMID: 27470974] (WB,, Mouse) WB Mouse
Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. PLoS Genet, 2015;11(11):e1005638. 2015 [PMID: 26528816] (IHC - paraffin,, Mouse) IHC - paraffin Mouse
Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model. J Neurosci, 2015;35(13):5409-21. 2015 [PMID: 25834064] (WB,, Rat) WB Rat
Aharon, Rotem, Janes, Peter W, Burgess, Anthony, Hamza, Kais, Klebaner, Fima, Lackmann, Martin A mathematical model for eph/ephrin-directed segregation of intermingled cells. PLoS ONE, 2014;9(12):e111803. 2014 [PMID: 25436892] (WB,, Human) WB Human
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014;9(1):e84179. 2014 [PMID: 24392113] (ICC Fluorescence,, Human) ICC Fluorescence Human
Show All 23 Publications.

Reviews for EphB2 Antibody (AF467) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Other.

Reviews using AF467:
Filter by Applications
Simple Western
(1)
WB
(2)
All Applications
Filter by Species
Mouse
(2)
Other
(1)
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Images Ratings Applications Species Date Details
Simple Western EphB2 AF467
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reviewed by:
Anonymous
Simple Western Mouse 07/12/2017
View

Summary

ApplicationSimple Western
Sample Testedmouse brain lysate
SpeciesMouse

Comments

CommentsSimple Western: anti-EphB2 antibody [biotechne AF467]
Simple Western lane view shows a specific 128 kDa signal for EphB2 in 0.5 mg/ml of mouse brain lysate (lysis buffer from ARY014 proteome profiler kit). This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50).
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21276420
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21276420

Details

Detection NotesSee PMID 21276420

Comments

CommentsPublished: See PMID 21276420Independent review from antibodyresource.com
 
reviewed by:
Anonymous
WB Other 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22144690
SpeciesOther

Blocking

Blocking DetailsSee PMID 22144690

Details

Detection NotesSee PMID 22144690

Comments

CommentsPublished: See PMID 22144690Independent review from antibodyresource.com

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
07/12/2017
Application: Simple Western
Species: Mouse

 
Anonymous
01/08/2015
Application: WB
Species: Mouse

 
Anonymous
01/08/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol EPHB2
Entrez
Uniprot