EphB2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at ...read more
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room ...read more
EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at ...read more
COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody ...read more
D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #AB-108-C, ...read more
EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) ...read more
EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

EphB2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763
Specificity
Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 5 Reviews rated 4.2
using
AF467 in the following applications:

Publications
Read Publications using
AF467 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB2 Antibody [Unconjugated]

  • CAPB
  • Cek5
  • Drt
  • DRTEphB2
  • EC 2.7.10
  • EC 2.7.10.1
  • EK5
  • elk-related tyrosine kinase
  • EPH receptor B2
  • eph tyrosine kinase 3
  • EphB2
  • EPH-like kinase 5
  • ephrin type-B receptor 2
  • EPHT3MGC87492
  • EPTH3
  • Erk
  • ERKHek5
  • Hek5
  • Nuk
  • PCBC
  • protein-tyrosine kinase HEK5
  • Qek2
  • Renal carcinoma antigen NY-REN-47
  • Sek3
  • Tyro5
  • Tyrosine-protein kinase receptor EPH-3
  • Tyrosine-protein kinase TYRO5

Background

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB500-138
Species: Hu, Mu, Rt, Ze
Applications: WB, IHC, IHC-P, Flow-IC
NBP2-37568
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB4640
Species: Hu
Applications: WB
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-81575
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00010598-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP1-68874
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-67358
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
MAB4540
Species: Hu, Mu, Rt
Applications: WB, IHC
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF

Publications for EphB2 Antibody (AF467)(33)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 9 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Neutralization, Simple Western, Western Blot.


Filter By Application
ICC
(3)
IHC
(6)
IHC-Fr
(1)
IHC-P
(3)
Immunoprecipitation
(6)
In Vivo
(1)
Neutralization
(1)
Simple Western
(1)
Western Blot
(10)
All Applications
Filter By Species
Human
(10)
Mouse
(19)
Rat
(2)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using AF467 Applications Species
A Kischel, C Audouard, MA Fawal, A Davy Ephrin-B2 paces neuronal production in the developing neocortex BMC Dev. Biol., 2020;20(1):12. 2020 [PMID: 32404061] (In Vivo, Mouse) In Vivo Mouse
ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020;11(5):307. 2020 [PMID: 32366833] (Western Blot, Human) Western Blot Human
D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands J. Leukoc. Biol., 2020;0(0):. 2020 [PMID: 32337793] (IHC, Human) IHC Human
L Farr, S Ghosh, N Jiang, K Watanabe, M Parlak, R Bucala, S Moonah CD74 signaling links inflammation to intestinal epithelial cell regeneration and promotes mucosal healing Cell Mol Gastroenterol Hepatol, 2020;0(0):. 2020 [PMID: 32004754] (IHC, Mouse) IHC Mouse
YL Liu, AM Horning, B Lieberman, M Kim, CK Lin, CN Hung, CW Chou, CM Wang, CL Lin, NB Kirma, MA Liss, R Vasisht, EP Perillo, K Blocher, H Horng, JA Taverna, J Ruan, TE Yankeelov, AK Dunn, TH Huang, HC Yeh, CL Chen Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer Cancers (Basel), 2019;11(12):. 2019 [PMID: 31805710] (Western Blot, Human) Western Blot Human
Z Zhang, M Ye, Q Li, Y You, H Yu, Y Ma, L Mei, X Sun, L Wang, W Yue, R Li, J Li, D Zhang The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling Cell Rep, 2019;29(1):49-61.e7. 2019 [PMID: 31577955] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
AS Ernst, LI Böhler, AM Hagenston, A Hoffmann, S Heiland, C Sticht, M Bendszus, M Hecker, H Bading, HH Marti, T Korff, R Kunze EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke Acta Neuropathol Commun, 2019;7(1):15. 2019 [PMID: 30722785] (Simple Western, Mouse) Simple Western Mouse
PN Mimche, CM Lee, SM Mimche, M Thapa, A Grakoui, M Henkemeyer, TJ Lamb EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells Sci Rep, 2018;8(1):2532. 2018 [PMID: 29416088] (ICC, Mouse) ICC Mouse
S Miura, A Suzuki Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28943029] (IHC, Mouse) IHC Mouse
YH Liu, JW Tsai, JL Chen, WS Yang, PC Chang, PL Cheng, DL Turner, Y Yanagawa, TW Wang, JY Yu Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon Sci Rep, 2017;7(0):42895. 2017 [PMID: 28276447] (ICC, Mouse) ICC Mouse
Show All 33 Publications.

Reviews for EphB2 Antibody (AF467) (5) 4.25

Average Rating: 4.2
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Mouse, Other.

Reviews using AF467:
Filter by Applications
WB
(3)
IP
(1)
Simple Western
(1)
All Applications
Filter by Species
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot EphB2 AF467
Enlarge
4
reviewed by:
STAVROULA THEOFANOUS
WB Mouse 07/05/2018
View

Summary

ApplicationWestern Blot
Sample Tested brain and spinal cord
SpeciesMouse
LotCFK071804A

Comments

CommentsBlot Loading
• ANTIBODY: Human/Mouse EphB2Antibody cat noAF542
Lane1: Precision Plus Protein™ Dual Color Standards7ul
Lane2:Ephb1 receptor (SC tissue from naive C57BL6j mouse)
Western blot experiment details
Gel:4-20% SDS page
Tranfer apparatus: Trans-Blot® Turbo™ Transfer System
Membrane: midi size nitrocellulose
Time: 25min at 75-125V
Primary ab:Goat –rat EphB1 Ab 1mg for 20h
Secondary ab: rabbit a-goat 1:5,000 for 1h
Substrate: HRP for 15min
Immunoprecipitation EphB2 AF467
Enlarge
4
reviewed by:
STAVROULA THEOFANOUS
IP Mouse 04/16/2018
View

Summary

ApplicationImmunoprecipitation
Sample Tested brain and spinal cord
SpeciesMouse
LotCVT0311121

Comments

CommentsBlot Loading
• ANTIBODY: Human/Mouse EphB2Antibody cat noAF467
Lane1: PreĐision Plus Protein™ Dual Color Standards8ul
Lane2:Ephb2 receptors IP 1mg protein (SC tissue)
Lane3:Ephb2 receptors no IP 25ug (SC tissue)
Lane4:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue)
Lane5:Ephb2 receptors IP 1mg (SC tissue)
Lane6:Ephb2 receptors no IP 25ug (SC tissue)
Lane7:Goat GFP IP (negative control instead of goat IgG)1mg (SC tissue)
Western blot experiment details
Gel:4-20% SDS page
Tranfer apparatus: Trans-Blot® Turďo™ Transfer System
Membrane: midi size nitrocellulose
Time: 25min at 75-125V
Primary ab:Goat –m/h EphB2 Ab 1mg for 20h
Secondary ab: donkey a-goat 1:15,000 for 1h
Substrate: HRP for 15min
Simple Western EphB2 AF467
Enlarge
5
reviewed by:
Anonymous
Simple Western Mouse 07/12/2017
View

Summary

ApplicationSimple Western
Sample Testedmouse brain lysate
SpeciesMouse

Comments

CommentsSimple Western: anti-EphB2 antibody [biotechne AF467]
Simple Western lane view shows a specific 128 kDa signal for EphB2 in 0.5 mg/ml of mouse brain lysate (lysis buffer from ARY014 proteome profiler kit). This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50).
  4
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21276420
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21276420

Details

Detection NotesSee PMID 21276420

Comments

CommentsPublished: See PMID 21276420Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
WB Other 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22144690
SpeciesOther

Blocking

Blocking DetailsSee PMID 22144690

Details

Detection NotesSee PMID 22144690

Comments

CommentsPublished: See PMID 22144690Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EphB2 Antibody (AF467) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF467

Additional EphB2 Products

Bioinformatics Tool for EphB2 Antibody (AF467)

Discover related pathways, diseases and genes to EphB2 Antibody (AF467). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EphB2 Antibody (AF467)

Discover more about diseases related to EphB2 Antibody (AF467).
 

Pathways for EphB2 Antibody (AF467)

View related products by pathway.

PTMs for EphB2 Antibody (AF467)

Learn more about PTMs related to EphB2 Antibody (AF467).

Blogs on EphB2.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.2
5
1
4
4
3
0
2
0
1
0

STAVROULA THEOFANOUS
07/05/2018
Application: WB
Species: Mouse

STAVROULA THEOFANOUS
04/16/2018
Application: IP
Species: Mouse

Anonymous
07/12/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol EPHB2
Uniprot