Caveolin-3 Antibody

Images

 
Western Blot: Caveolin-3 Antibody [NB110-5029] - Analysis of rat cardiac muscle protein extract.
Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - C2C11 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Caveolin ...read more
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse skeletal muscle tissue.
Flow Cytometry: Caveolin-3 Antibody [NB110-5029] - Analysis of U-87 MG cells.
Immunocytochemistry/ Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - HeLa cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a polyclonal ...read more
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse heart tissue.
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse lymph node tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Caveolin-3 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3
Marker
Muscle Specific Caveolae Marker, T-tubule Marker
Specificity
Detects caveolin-3 from rat tissues. This does not detect caveolin-1 or -2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAV3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:100 - 200
  • Immunohistochemistry-Paraffin 1:100 - 200
  • Immunoprecipitation 5 ug
Application Notes
WB: Detects an approx. 21 kDa protein representing caveolin-3 from rat heart homogenate.
Control
Caveolin-3 Overexpression Lysate
Publications
Read Publications using
NB110-5029 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 14688207). Rat reactivity reported in scientific literature (PMID: 11567040).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caveolin-3 Antibody

  • CAV3
  • caveolin 3
  • Caveolin3
  • Caveolin-3
  • LGMD1C
  • LGMD1CMGC126100
  • LQT9
  • LQT9MGC126101
  • M-caveolin
  • MGC126129
  • VIP21
  • VIP-21

Background

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caveolin-3 Antibody (NB110-5029)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caveolin-3 Antibody (NB110-5029) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol CAV3
Entrez
Uniprot