Western Blot: Beta-actin Antibody[NB110-67828]- Chicken fibroblast cells probed with monoclonal PA to Beta Actin, clone AC-15 (0.5 ug/ml). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP ...read more
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beta-Actin Antibody (AC-15) Summary
Slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
N-terminus of the beta isoform of actin.
Beta-actin (AC-15). In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
This is the purified version of NB600-501. Useful as an extract loading control for a wide variety of cell extracts in Western blot. The antibody is not suitable for use in formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives). Frozen sections are suitable. Antibody may also be used for Immunocytochemistry/Immunofluorescence and Immunoprecipitation
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB110-67828 in the following applications:
The antibody reacts with b-actin-expressing cells in bovine, canine, chicken, feline, carp, leech, guinea pig, human, mouse, porcine, rabbit, rat, and sheetp. Does not react with amoeba or drosophila. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM PBS (pH 7.4)
15mM Sodium Azide
Protein A or G purified
This is the purified version of NB600-501.
Alternate Names for beta-Actin Antibody (AC-15)
actin, cytoplasmic 1
beta cytoskeletal actin
PS1TP5-binding protein 1
This is an ideal reagent for use as a cell extract loading control on Western blot. It has been used as such in most of the references below. Molecular weight of beta actin is 42kD.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for beta-Actin Antibody (NB110-67828). (Showing 1 - 4 of 4 FAQs).
Why is beta actin used as a loading control in blotting ?
Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Are the beta-Actin antibodies validated in Simple Western?
Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Do Beta-Actin antibodies come in lyophilized format?
ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
What the theoretical molecular weight for Beta-Actin antibodies?
The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post.
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