BDNF Antibody - Azide and BSA Free

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat DRG at 40 ug/ml concentration using Rabbit antibody to mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin): IgG , sheep antibody to neurofilament (in green), DAPI counterstained appearing in blue.

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat DRG at 40 ug/ml concentration using Rabbit antibody to mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin): IgG , sheep antibody to neurofilament, DAPI counterstained.

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat DRG at 40 ug/ml concentration using Rabbit antibody to mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin): IgG

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat DRG using sheep antibody to neurofilament

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat RG at 40 ug/ml concentration using Rabbit antibody to mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin): IgG and DAPI counterstained

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

BDNF Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide from n-terminal region of human mature BDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebra fish and xenopus.
• Specificity: mature BDNF.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDNF
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Control:

  BDNF Overexpression Lysate

Reactivity Notes

The peptide is homologous in many other species including Zebrafish and Xenopus.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for BDNF Antibody - Azide and BSA Free

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (NB100-98683)(1)

Publication using NB100-98683

• Lin JB, Lin NL, Li X, Kang MQ Antagonist of Chrna1 prevents the pathogenesis of primary focal hyperhidrosis Annals of clinical and translational neurology 2022-04-08 [PMID: 35393764] (ELISA)

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)
  • 12064(Mouse)
  • 24225(Rat)
• Uniprot:
  • P23560()