TrkC Antibody [Unconjugated]

Western blot shows lysates of mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkC at approximately 100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. Specific bands were detected for TrkC at approximately 111 and 159 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Mu, Rt
• Applications: WB, Simple Western, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TrkC Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TrkC
  Cys32-Thr429
  Accession # Q6VNS1
• Specificity: Detects mouse TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) TrkC is observed and less than 2% cross‑reactivity with recombinant mouse TrkB, recombinant rat TrkA, and rhTrkA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTRK3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkC Antibody [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• ETV6-NTRK3 fusion
• gp145(trkC)
• GP145-TrkC
• Neurotrophic tyrosine kinase receptor type 3
• neurotrophic tyrosine kinase, receptor, type 3
• NT 3 receptor
• NT-3 growth factor receptor
• NTRK3
• TrkC tyrosine kinase
• TrkC
• trk-C
• TRKCneurotrophin 3 receptor
• tyrosine kinase receptor C

Background

The neurotrophins, including NGF, BDNF, NT‑3 and NT‑4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT‑3 and NT‑4/5, and TrkC only binds NT‑3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
2. Dechant, G. (2001) Cell Tissue Res. 305:229.
3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
6. Shelton, D. et al. (1995) J. Neurosci. 15:477.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkC Antibody (AF1404)(39)

Publications using AF1404

• N Sultan, BA Scheven Exploring the potential application of dental pulp stem cells in neuroregenerative medicine Neural regeneration research, 2022;17(4):775-776. 2022 [PMID: 34472466] (Neutralization, Rat)
• N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven Dental pulp stem cells stimulate neuronal differentiation of PC12 cells Neural regeneration research, 2021;16(9):1821-1828. 2021 [PMID: 33510089] (Neutralization, Rat)
• V Kampanis, B Tolou-Dabb, L Zhou, W Roth, R Puttagunta Cyclic Stretch of Either PNS or CNS Located Nerves Can Stimulate Neurite Outgrowth Cells, 2020;10(1):. 2020 [PMID: 33379276] (ICC, Rat)
• A Escalante, R Klein Spinal Inhibitory Ptf1a-Derived Neurons Prevent Self-Generated Itch Cell Rep, 2020;33(8):108422. 2020 [PMID: 33238109] (IHC, Mouse)
• L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020;11(1):4175. 2020 [PMID: 32826903] (IHC, Mouse)
• L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020;11(1):4175. 2020 [PMID: 32826903] (IHC, Mouse)
• D Xiao, Q Deng, Y Guo, X Huang, M Zou, J Zhong, P Rao, Z Xu, Y Liu, Y Hu, Y Shen, K Jin, M Xiang Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts Sci Adv, 2020;6(22):eaaz5858. 2020 [PMID: 32523990] (ICC, Mouse)
• P Pagella, J Catón, CT Meisel, TA Mitsiadis Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons Cells, 2020;9(3):. 2020 [PMID: 32155948] (IHC-P, Human)
• M Bohic, I Marics, C Santos, P Malapert, N Ben-Arie, C Salio, A Reynders, Y Le Feuvre, AJ Saurin, A Moqrich Loss of bhlha9 Impairs Thermotaxis and Formalin-Evoked Pain in a Sexually Dimorphic Manner Cell Rep, 2020;30(3):602-610.e6. 2020 [PMID: 31968239] (IF, Mouse)
• Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019;10(1):4137. 2019 [PMID: 31515492] (ICC, Mouse)
• D Acton, X Ren, S Di Costanz, A Dalet, S Bourane, I Bertocchi, C Eva, M Goulding Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch Cell Rep, 2019;28(3):625-639.e6. 2019 [PMID: 31315043] (IHC-Fr, Mouse)
• S Wiszniak, Q Schwarz Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 2019;20(1):21. 2019 [PMID: 31036074] (IHC-F, Mouse)
• A Takeoka, S Arber Functional Local Proprioceptive Feedback Circuits Initiate and Maintain Locomotor Recovery after Spinal Cord Injury Cell Rep, 2019;27(1):71-85.e3. 2019 [PMID: 30943416] (IHC, Mouse)
• S Desiderio, S Vermeiren, C Van Campen, S Kricha, E Malki, S Richts, EV Fletcher, T Vanwelden, BZ Schmidt, KA Henningfel, T Pieler, CG Woods, V Nagy, C Verfaillie, EJ Bellefroid Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA Cell Rep, 2019;26(13):3522-3536.e5. 2019 [PMID: 30917309] (ICC, Mouse)
• L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019;26(13):3484-3492.e4. 2019 [PMID: 30917305] (IHC, Mouse)
• M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019;26(6):1419-1431.e4. 2019 [PMID: 30726728] (IHC, Mouse)
• MJ Patil, AH Hovhannisy, AN Akopian Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines PLoS ONE, 2018;13(6):e0198601. 2018 [PMID: 29864146] (IHC, Mouse)
• R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats eNeuro, 2018;5(1):. 2018 [PMID: 29468190] (IHC, Rat)
• AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells Sci Rep, 2018;8(1):603. 2018 [PMID: 29330377] (ICC, Human)
• YA Bernal Sie, J Haseleu, A Kozlenkov, V Bégay, GR Lewin Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System Front Mol Neurosci, 2017;10(0):70. 2017 [PMID: 28360836] (IHC, Mouse)
• EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway J. Neurosci. Methods, 2017;282(0):34-42. 2017 [PMID: 28279735] (ELISA Development (Capture), Rat)
• L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain Sci Rep, 2017;7(0):43493. 2017 [PMID: 28240741] (IHC-Fr, Mouse)
• Ariadna Arbat-Plan Endogenous Modulation of Trkb Signaling by Treadmill Exercise After Peripheral Nerve Injury Neuroscience, 2016;0(0):. 2016 [PMID: 27984178] (Western Blot, Rat)
• Tropomyosin Receptor Kinase C Targeted Delivery of a Peptidomimetic Ligand-Photosensitizer Conjugate Induces Antitumor Immune Responses Following Photodynamic Therapy Sci Rep, 2016;6(0):37209. 2016 [PMID: 27853305] (Neutralization, Mouse)
• EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes J Neurosci, 2016;36(15):4362-76. 2016 [PMID: 27076431] (IHC-Fr, Mouse)
• Gay M, Valenta T, Herr P, Paratore-Hari L, Basler K, Sommer L Distinct adhesion-independent functions of beta-catenin control stage-specific sensory neurogenesis and proliferation. BMC Biol, 2015;13(0):24. 2015 [PMID: 25885041] (IHC, Mouse)
• Oliveira Fernandes M, Tourtellotte W Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis. J Neurosci, 2015;35(14):5566-78. 2015 [PMID: 25855173] (IHC-Fr, Mouse)
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2015;18(1):145-53. 2015 [PMID: 25420068] (IHC, Mouse)
• Romi E, Gokhman I, Wong E, Antonovsky N, Ludwig A, Sagi I, Saftig P, Tessier-Lavigne M, Yaron A ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A. Nat Commun, 2014;5(0):4058. 2014 [PMID: 24898499] (IHC, Mouse)
• Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor. PLoS Genet, 2014;10(2):e1004081. 2014 [PMID: 24516396] (IHC, Mouse)
• Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013;33(12):5399-410. 2013 [PMID: 23516305] (IHC-Fr, Mouse)
• Positional differences of axon growth rates between sensory neurons encoded by Runx3. EMBO J., 2012;31(18):3718-29. 2012 [PMID: 22903063] (IHC-Fr, Mouse)
• Huettl RE, Soellner H, Bianchi E, Novitch BG, Huber AB Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb. PLoS Biol., 2011;9(2):e1001020. 2011 [PMID: 21364975] (IHC, Mouse)
• Demel C, Hoegen T, Giese A Reduced spiral ganglion neuronal loss by adjunctive neurotrophin-3 in experimental pneumococcal meningitis. J Neuroinflammation, 2011;8(1):7. 2011 [PMID: 21261959] (IHC-P, Mouse)
• Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2011;14(1):54-61. 2011 [PMID: 21102451] (IHC-Fr, Mouse)
• Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis. J. Clin. Invest., 2010;120(3):850-8. 2010 [PMID: 20160348] (Neutralization, Human)
• Senzaki K, Ozaki S, Yoshikawa M, Ito Y, Shiga T Runx3 is required for the specification of TrkC-expressing mechanoreceptive trigeminal ganglion neurons. Mol. Cell. Neurosci., 2010;43(3):296-307. 2010 [PMID: 20034568] (IHC, Mouse)
• Kawamata T, Ninomiya T, Toriyabe M, Yamamoto J, Niiyama Y, Omote K, Namiki A Immunohistochemical analysis of acid-sensing ion channel 2 expression in rat dorsal root ganglion and effects of axotomy. Neuroscience, 2006;143(1):175-87. 2006 [PMID: 16949762] (IHC, Rat)
• Tamura S, Morikawa Y, Senba E TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons. Neuroscience, 2005;130(1):223-8. 2005 [PMID: 15561438] (ICC, Mouse)

Review for TrkC Antibody (AF1404) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23516305
• Species: Mouse

Bioinformatics

• Gene Symbol: NTRK3
• Uniprot:
  • Q6VNS1()