TrkC Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. Specific bands were detected for TrkC at approximately 111 and 159 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse/Rat ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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TrkC Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TrkC
Cys32-Thr429
Accession # Q6VNS1
Specificity
Detects mouse TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) TrkC is observed and less than 2% cross‑reactivity with recombinant mouse TrkB, recombinant rat TrkA, and rhTrkA is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NTRK3
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 4
using
AF1404 in the following applications:

Publications
Read Publications using
AF1404 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkC Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
  • ETV6-NTRK3 fusion
  • gp145(trkC)
  • GP145-TrkC
  • Neurotrophic tyrosine kinase receptor type 3
  • neurotrophic tyrosine kinase, receptor, type 3
  • NT 3 receptor
  • NT-3 growth factor receptor
  • NTRK3
  • TrkC tyrosine kinase
  • TrkC
  • trk-C
  • TRKCneurotrophin 3 receptor
  • tyrosine kinase receptor C

Background

The neurotrophins, including NGF, BDNF, NT‑3 and NT‑4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT‑3 and NT‑4/5, and TrkC only binds NT‑3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
  6. Shelton, D. et al. (1995) J. Neurosci. 15:477.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TrkC Antibody (AF1404)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: ELISA Capture, ICC, IHC, IHC - Frozen, IHC Fresh/Frozen, IHC Frozen, IHC OCT-embedded, IHC Paraffin-embedded, IHC-F, In vivo, Neut, WB.


Filter By Application
ELISA Capture
(1)
ICC
(2)
IHC
(7)
IHC - Frozen
(1)
IHC Fresh/Frozen
(1)
IHC Frozen
(4)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(1)
IHC-F
(1)
In vivo
(1)
Neut
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(17)
Rat
(3)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF1404 Applications Species
MJ Patil, AH Hovhannisy, AN Akopian Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines PLoS ONE, 2018;13(6):e0198601. 2018 [PMID: 29864146] (IHC,, Mouse) IHC Mouse
AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells Sci Rep, 2018;8(1):603. 2018 [PMID: 29330377] (ICC,, Human) ICC Human
YA Bernal Sie, J Haseleu, A Kozlenkov, V Bégay, GR Lewin Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System Front Mol Neurosci, 2017;10(0):70. 2017 [PMID: 28360836] (IHC-F,, Mouse) IHC-F Mouse
EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway J. Neurosci. Methods, 2017;282(0):34-42. 2017 [PMID: 28279735] (ELISA Capture,, Rat) ELISA Capture Rat
L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain Sci Rep, 2017;7(0):43493. 2017 [PMID: 28240741] (IHC Frozen,, Mouse) IHC Frozen Mouse
Ariadna Arbat-Plan Endogenous Modulation of Trkb Signaling by Treadmill Exercise After Peripheral Nerve Injury Neuroscience, 2016;0(0):. 2016 [PMID: 27984178] (WB,, Rat) WB Rat
Tropomyosin Receptor Kinase C Targeted Delivery of a Peptidomimetic Ligand-Photosensitizer Conjugate Induces Antitumor Immune Responses Following Photodynamic Therapy Sci Rep, 2016;6(0):37209. 2016 [PMID: 27853305] (In vivo,, Mouse) In vivo Mouse
EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes J Neurosci, 2016;36(15):4362-76. 2016 [PMID: 27076431] (IHC - Frozen,, Mouse) IHC - Frozen Mouse
Gay M, Valenta T, Herr P, Paratore-Hari L, Basler K, Sommer L Distinct adhesion-independent functions of beta-catenin control stage-specific sensory neurogenesis and proliferation. BMC Biol, 2015;13(0):24. 2015 [PMID: 25885041] (IHC,, Mouse) IHC Mouse
Oliveira Fernandes M, Tourtellotte W Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis. J Neurosci, 2015;35(14):5566-78. 2015 [PMID: 25855173] (IHC Fresh/Frozen,, Mouse) IHC Fresh/Frozen Mouse
Show All 22 Publications.

Review for TrkC Antibody (AF1404) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1404:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23516305
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23516305

Details

Detection NotesSee PMID 23516305

Comments

CommentsSee PMID 23516305

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Blogs on TrkC.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Recent Reviews

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Anonymous
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NTRK3
Uniprot