Recombinant Human NT-4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human NT-4 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.3‑3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-4 protein
Gly81-Ala210
Accession #
N-terminal Sequence
Gly81
Protein/Peptide Type
Recombinant Proteins
Gene
NTF4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
268-N4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-4 Protein

  • GLC10
  • GLC1O
  • neurotrophic factor 4
  • neurotrophic factor 5
  • neurotrophin 4
  • neurotrophin 5 (neurotrophin 4/5)
  • neurotrophin-4
  • neurotrophin-5
  • Neutrophic factor 4
  • NT4
  • NT-4
  • NT-4/5
  • NT-5
  • NTF4
  • NTF5
  • NTF5NT5

Background

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1-3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48-52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8-10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8-10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14-16).

  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

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Publications for NT-4 (268-N4)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(3)
Cell Culture
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 268-N4 Applications Species
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020;9(9):. 2020 [PMID: 32839392] (Cell Culture, Human) Cell Culture Human
N Michel, P Narayanan, O Shomroni, M Schmidt Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction Cell Rep, 2020;32(3):107912. 2020 [PMID: 32697985] (Bioassay, Mouse) Bioassay Mouse
M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo Int J Nanomedicine, 2019;14(0):6117-6131. 2019 [PMID: 31534337] (In Vivo, Mouse) In Vivo Mouse
Samah B, Porcheray F, Gras G Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 2008;151(3):476-86. 2008 [PMID: 18190610] (Bioassay, Human) Bioassay Human
Royo NC, Lebold D, Magge SN, Chen I, Hauspurg A, Cohen AS, Watson DJ Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function. Neuroscience, 2007;148(2):359-70. 2007 [PMID: 17681695] (In Vivo, Rat) In Vivo Rat
Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Chem. Biol., 2006;13(5):549-56. 2006 [PMID: 16720276] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol NTF4
Uniprot