TrkB Antibody [Unconjugated]

Images

 
TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue ...read more
Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen ...read more
Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TrkB Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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TrkB Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TrkB
Cys32-His429
Accession # P15209
Specificity
Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) TrkB is observed, and less than 2% cross-reactivity with rhTrkA, recombinant rat TrkA, and recombinant mouse TrkC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NTRK2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.5 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 9 Reviews rated 4.7
using
AF1494 in the following applications:

Publications
Read Publications using
AF1494 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkB Antibody [Unconjugated]

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC 2.7.10.1
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B

Background

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3, and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TrkB Antibody (AF1494)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 7 applications: ELISA Capture, ELISA Development (Capture), ICC, IHC, IHC-F, IHC-Fr, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development (Capture)
(1)
ICC
(4)
IHC
(8)
IHC-F
(1)
IHC-Fr
(5)
Western Blot
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(19)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF1494 Applications Species
P Gómez-Fern, A Lopez de L, I Astobiza, J Mena, A Urtasun, V Altmann, F Matesanz, D Otaegui, E Urcelay, A Antigüedad, S Malhotra, X Montalban, T Castillo-T, L Espino-Pai, O Aktas, M Buttmann, A Chan, B Fontaine, PA Gourraud, M Hecker, S Hoffjan, C Kubisch, T Kümpfel, F Luessi, UK Zettl, F Zipp, I Alloza, M Comabella, CM Lill, K Vandenbroe The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis Cells, 2020;9(1):. 2020 [PMID: 31936765] (Western Blot, Mouse) Western Blot Mouse
GJ Moon, S Kim, MT Jeon, KJ Lee, IS Jang, M Nakamura, SR Kim Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer&#039;s Disease J Clin Med, 2019;8(12):. 2019 [PMID: 31766645] (IHC, Mouse) IHC Mouse
SM Fred, L Laukkanen, CA Brunello, L Vesa, H Göös, I Cardon, R Moliner, T Maritzen, M Varjosalo, PC Casarotto, E Castrén Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2 J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31631060] (ELISA Capture, Mouse) ELISA Capture Mouse
Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019;10(1):4137. 2019 [PMID: 31515492] (ICC, Mouse) ICC Mouse
D Acton, X Ren, S Di Costanz, A Dalet, S Bourane, I Bertocchi, C Eva, M Goulding Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch Cell Rep, 2019;28(3):625-639.e6. 2019 [PMID: 31315043] (IHC-Fr, Mouse) IHC-Fr Mouse
S Wiszniak, Q Schwarz Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 2019;20(1):21. 2019 [PMID: 31036074] (IHC-F, Human) IHC-F Human
A Takeoka, S Arber Functional Local Proprioceptive Feedback Circuits Initiate and Maintain Locomotor Recovery after Spinal Cord Injury Cell Rep, 2019;27(1):71-85.e3. 2019 [PMID: 30943416] (IHC, Mouse) IHC Mouse
S Desiderio, S Vermeiren, C Van Campen, S Kricha, E Malki, S Richts, EV Fletcher, T Vanwelden, BZ Schmidt, KA Henningfel, T Pieler, CG Woods, V Nagy, C Verfaillie, EJ Bellefroid Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA Cell Rep, 2019;26(13):3522-3536.e5. 2019 [PMID: 30917309] (ICC, Human) ICC Human
L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019;26(13):3484-3492.e4. 2019 [PMID: 30917305] (IHC, Mouse) IHC Mouse
M Zhang, Y Wang, J Geng, S Zhou, B Xiao Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice Cell Rep, 2019;26(6):1419-1431.e4. 2019 [PMID: 30726728] (IHC, Mouse) IHC Mouse
Show All 24 Publications.

Reviews for TrkB Antibody (AF1494) (9) 4.79

Average Rating: 4.7
(Based on 9 reviews)
We have 9 reviews tested in 1 species: Mouse.

Reviews using AF1494:
Filter by Applications
WB
(3)
IHC-Fr
(2)
IF
(1)
IP
(2)
ICC
(1)
All Applications
Filter by Species
Mouse
(9)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23516305
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23516305

Details

Detection NotesSee PMID 23516305

Comments

CommentsPublished PMID 23516305
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23224557
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23516305
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23516305

Details

Detection NotesSee PMID 23516305

Comments

CommentsSee PMID 23516305
 
reviewed by:
Anonymous
IP Mouse 12/19/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedCultured hippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
WB Mouse 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedCultured hippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IP Mouse 12/19/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedTrkB from hippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IHC-Fr Mouse 12/19/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHippocampal cryosections
SpeciesMouse
 
reviewed by:
Anonymous
ICC Mouse 12/19/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHippocampal neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
Western Blot TrkB AF1494
Enlarge
reviewed by:
Anonymous
WB Mouse 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedHippocampus tissues
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

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Video Protocols

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FAQs for TrkB Antibody (AF1494) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1494

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PTMs for TrkB Antibody (AF1494)

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Blogs on TrkB.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Recent Reviews

4.7
5
7
4
1
3
1
2
0
1
0

 
Anonymous
01/05/2015
Application: WB
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NTRK2
Uniprot