Recombinant Human beta-NGF Protein


Recombinant Human beta -NGF (Catalog # 256‑GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2‑2 ng/mL.
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

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Recombinant Human beta-NGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2‑2 ng/mL.
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
13.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
Read Publications using
256-GF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428


NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and  gamma . Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Publications for beta-NGF (256-GF)(57)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Horse, Porcine.

We have publications tested in 9 applications: Bioassay, Cell Culture, Control, Differentiation, ELISA (Capture), ELISA (Standard), ELISA Standard, In Vivo, Western Blot.

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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 256-GF Applications Species
Bai, Z;Bartelo, N;Aslam, M;Hale, C;Blachere, NE;Parveen, S;Spolaore, E;DiCarlo, E;Gravallese, E;Smith, MH;Accelerating Medicines Partnership Program: Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Network, ;Frank, MO;Jiang, CS;Zhang, H;Lewis, MJ;Sikandar, S;Pitzalis, C;Malfait, AM;Miller, RE;Zhang, F;Goodman, S;Darnell, R;Wang, F;Orange, DE; Machine Learning Reveals Synovial Fibroblast Genes Associated with Pain Affect Sensory Nerve Growth in Rheumatoid Arthritis medRxiv : the preprint server for health sciences 2023-08-23 [PMID: 37662384] (Bioassay, Mouse) Bioassay Mouse
Held, A;Adler, M;Marques, C;Reyes, CJ;Kavuturu, AS;Quadros, ARAA;Ndayambaje, IS;Lara, E;Ward, M;Lagier-Tourenne, C;Wainger, BJ; iPSC motor neurons, but not other derived cell types, capture gene expression changes in postmortem sporadic ALS motor neurons Cell reports 2023-08-30 [PMID: 37651231] (Cell Culture, Human) Cell Culture Human
Holland, SM;Gallo, G; Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics Journal of cell science 2023-08-03 [PMID: 37534394] (Cell Culture) Cell Culture
Onda-Ohto, A;Hasegawa-Ogawa, M;Matsuno, H;Shiraishi, T;Bono, K;Hiraki, H;Kanegae, Y;Iguchi, Y;Okano, HJ; Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress Molecular brain 2023-07-26 [PMID: 37496071] (Bioassay, Human) Bioassay Human
Go, YY;Lee, CM;Chae, SW;Song, JJ; Regenerative capacity of trophoblast stem cell-derived extracellular vesicles on mesenchymal stem cells Biomaterials research 2023-06-27 [PMID: 37370189] (Bioassay, Human) Bioassay Human
Mantuano, E;Zampieri, C;Azmoon, P;Gunner, CB;Heye, KR;Gonias, SL; An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein JCI insight 2023-06-27 [PMID: 37368488] (Cell Culture, Mouse) Cell Culture Mouse
Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS; IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries Cells 2023-05-25 [PMID: 37296600] (Bioassay, Human) Bioassay Human
Walsh, J;Palandra, J;Goihberg, E;Deng, S;Hurst, S;Neubert, H; Analysis of ?-nerve growth factor and its precursor during human pregnancy by immunoaffinity-liquid chromatography tandem mass spectrometry Scientific reports 2023-06-06 [PMID: 37280257] (Human) Human
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 2022-08-22;151(0):156-163. 2022-08-22 [PMID: 36029605] (Control, Horse) Control Horse
Show All 57 Publications.

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Gene Symbol NGF