Recombinant Human beta-NGF Protein

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1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human beta -NGF (Catalog # 256‑GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2‑2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human beta-NGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2‑2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Ser122-Ala241
Accession #
N-terminal Sequence
Ser122
Protein/Peptide Type
Recombinant Proteins
Gene
NGF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
256-GF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and  gamma. Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

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256-GF
Species: Hu
Applications: Bioactivity

Publications for beta-NGF (256-GF)(32)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Porcine.

We have publications tested in 4 applications: Bioassay, ELISA (Capture), ELISA Standard, Western Blot.


Filter By Application
Bioassay
(26)
ELISA (Capture)
(1)
ELISA Standard
(1)
Western Blot
(2)
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Filter By Species
Human
(18)
Mouse
(1)
Rat
(3)
Canine
(2)
Chicken
(3)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using 256-GF Applications Species
D Baumeister, W Eich, S Saft, O Geisel, R Hellweg, A Finn, CI Svensson, J Tesarz No evidence for altered plasma NGF and BDNF levels in fibromyalgia patients Sci Rep, 2019;9(1):13667. 2019 [PMID: 31541132] (ELISA Standard, Human) ELISA Standard Human
A Kizilyer, MV Singh, VB Singh, S Suwunnakor, J Palis, SB Maggirwar Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis Sci Rep, 2019;9(1):2781. 2019 [PMID: 30808933] (Bioassay, Human) Bioassay Human
AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics Sci Rep, 2019;9(1):930. 2019 [PMID: 30700786] (ELISA (Capture), Human) ELISA (Capture) Human
PM Deardorff, TB McKay, S Wang, CE Ghezzi, DM Cairns, RD Abbott, JL Funderburg, KR Kenyon, DL Kaplan Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System Sci Rep, 2018;8(1):17294. 2018 [PMID: 30470798] (Bioassay, Human) Bioassay Human
K Kirino, T Nakahata, T Taguchi, MK Saito Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition Sci Rep, 2018;8(1):12865. 2018 [PMID: 30150715] (Bioassay, Human) Bioassay Human
T Miladinovi, RG Ungard, K Linher-Mel, S Popovic, G Singh Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain Mol Pain, 2018;14(0):1744806918776. 2018 [PMID: 29761734] (Bioassay, Human) Bioassay Human
DR Gomez, LA Byers, M Nilsson, L Diao, J Wang, L Li, P Tong, M Hofstad, B Saigal, I Wistuba, N Kalhor, S Swisher, Y Fan, WK Hong, M Suraokar, C Behrens, C Moran, JV Heymach Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer Oncotarget, 2018;9(18):14268-14284. 2018 [PMID: 29581842] (Bioassay, Human) Bioassay Human
JW Martin, JC Chen, J Neidleman, K Tatsumi, J Hu, LC Giudice, WC Greene, NR Roan Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma Biol. Reprod., 2018;0(0):. 2018 [PMID: 29518187] (Bioassay, Human) Bioassay Human
KC Chun, SH Lee, JW Kim, EJ Jin, KM Kim, CH Chun Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts J Orthop Surg Res, 2017;12(1):93. 2017 [PMID: 28615040] (Bioassay, Human) Bioassay Human
Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo Sci Rep, 2016;6(0):38644. 2016 [PMID: 27995962] (Bioassay, Rat) Bioassay Rat
Show All 32 Publications.

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Bioinformatics

Gene Symbol NGF
Uniprot