TrkA Antibody [Unconjugated]

Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical characterization of ChR2-expressing DRG neurons. A, Immunolabeling of ChR2+ neurons (Venus; green) with either NF200, TrkC, CGRP, TrkA, TRPV1, or IB4 (red) in the L4 DRG contralateral and ipsilateral to PNI on day 14. B, Percentage of colocalization of each marker in ChR2+ DRG neurons [n = 4 rats (two to three slices per rat)]. Size-frequency histogram (inset) illustrating the distribution of the cross-sectional areas of ChR2+ NF200+ DRG neurons in the L4 DRG ipsilateral (371 cells) and contralateral (316 cells) to PNI on day 14. Values represent mean ± SEM. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29468190), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TrkA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TrkA
  Ala33-Pro418
  Accession # P35739
• Specificity: Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTRK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkA Antibody [Unconjugated]

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
2. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkA Antibody (AF1056)(51)

Publications using AF1056

• Abdullah, NS;Bradaia, A;Defaye, M;Ohland, C;Svendsen, K;Dickemann, A;Delanne-Cumenal, M;Hassan, A;Iftinca, M;McCoy, KD;Altier, C; Early life microbiota colonization programs nociceptor sensitivity by regulating NGF production in mast cells Mucosal immunology 2024-12-09 [PMID: 39662673] (Immunohistochemistry, Mouse)
• Tanaka, T;Isonishi, A;Banja, M;Yamamoto, R;Sonobe, M;Okuda-Ashitaka, E;Furue, H;Okuda, H;Tatsumi, K;Wanaka, A; Dermal macrophages control tactile perception under physiological conditions via NGF signaling Scientific reports 2024-11-08 [PMID: 39516548] (Immunohistochemistry, Transgenic Mouse)
• Koui, Y;Song, S;Dong, X;Mukouyama, YS; Local keratinocyte-nociceptor interactions enhance obesity-mediated small fiber neuropathy via NGF-TrkA-PI3K signaling axis bioRxiv : the preprint server for biology 2024-07-16 [PMID: 39372742] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Cameron, B;Torres-Hernandez, L;Montague, VL;Lewis, KA;Smith, H;Fox, J;Guo, X;Kalb, RG;George, L; Titin is a nucleolar protein in neurons Research square 2024-03-04 [PMID: 38496572] (Immunohistochemistry, Mouse)
• Fitzsimons, LA;Staurengo-Ferrari, L;Bogen, O;Araldi, D;Bonet, IJM;Jordan, EE;Levine, JD;Tucker, KL; The Primary Cilium and its Hedgehog Signaling in Nociceptors Contribute to Inflammatory and Neuropathic Pain Research square 2024-02-26 [PMID: 38464172] (Immunohistochemistry, Rat)
• N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy Cell Reports, 2023-03-21;42(4):112295. 2023-03-21 [PMID: 36947543] (IHC, Mouse)
• Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al. Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold Neuron 1/4/2023 [PMID: 36323322]
• DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain Oncogene, 2022-06-02;12(1):9241. 2022-06-02 [PMID: 35655086] (IHC, Human)
• K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch Nature Communications, 2022-05-02;13(1):2367. 2022-05-02 [PMID: 35501343] (IHC, Mouse)
• NJ Yang, J Isensee, DV Neel, AU Quadros, HB Zhang, J Lauzadis, SM Liu, S Shiers, A Belu, S Palan, S Marlin, J Maignel, A Kennedy-Cu, VS Tong, M Moayeri, P Röderer, A Nitzsche, M Lu, BL Pentelute, O Brüstle, V Tripathi, KA Foster, TJ Price, RJ Collier, SH Leppla, M Puopolo, BP Bean, TM Cunha, T Hucho, IM Chiu Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons Nature Neuroscience, 2021-12-20;0(0):. 12/20/2021 [PMID: 34931070]
• Vladimir Vladimirovich Muzyka, Tudor Constantin Badea Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification Neural Development 12/1/2021 [PMID: 34548095]
• Mark A. Landy, Megan Goyal, Helen C. Lai Nociceptor subtypes are born continuously over DRG development. Developmental Biology 11/1/2021 [PMID: 34352273]
• S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons Frontiers in Physiology, 2021-07-15;12(0):644929. 2021-07-15 [PMID: 34335288] (IHC, Rat)
• K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021-04-07;146(3):125-135. 2021-04-07 [PMID: 34030795] (IHC, Rat)
• MA Landy, M Goyal, KM Casey, C Liu, HC Lai Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation Cell Reports, 2021-03-30;34(13):108913. 2021-03-30 [PMID: 33789102] (IHC, Mouse)
• M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone Molecular pain, 2020-01-01;16(0):1744806920975. 2020-01-01 [PMID: 33280501] (Western Blot, Rat)
• Larissa de Clauser, Ana P. Luiz, Sonia Santana-Varela, John N. Wood, Shafaq Sikandar Sensitization of Cutaneous Primary Afferents in Bone Cancer Revealed by In Vivo Calcium Imaging Cancers (Basel) 11/24/2020 [PMID: 33255209]
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020-11-24;33(8):108425. 2020-11-24 [PMID: 33238113] (IHC, Rat)
• L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020-08-21;11(1):4175. 2020-08-21 [PMID: 32826903] (IHC, Mouse)
• M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain J Neuroinflammation, 2020-06-12;17(1):183. 2020-06-12 [PMID: 32532285] (IHC, Rat)
• Yiqiao Wang, Haohao Wu, Pavel Zelenin, Paula Fontanet, Simone Wanderoy, Charles Petitpré et al. Muscle-selective RUNX3 dependence of sensorimotor circuit development Development 10/15/2019 [PMID: 31575648]
• Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019-09-12;10(1):4137. 2019-09-12 [PMID: 31515492] (ICC, Mouse)
• X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity Cell Rep, 2019-06-25;27(13):3799-3807.e3. 2019-06-25 [PMID: 31242414] (IHC, Mouse)
• Alex Bersellini Farinotti, Gustaf Wigerblad, Diana Nascimento, Duygu B. Bas, Carlos Morado Urbina, Kutty Selva Nandakumar et al. Cartilage-binding antibodies induce pain through immune complex–mediated activation of neurons Journal of Experimental Medicine 8/5/2019 [PMID: 31196979]
• S Wiszniak, Q Schwarz Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 2019-04-29;20(1):21. 2019-04-29 [PMID: 31036074] (IHC-F, Mouse)
• L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019-03-26;26(13):3484-3492.e4. 2019-03-26 [PMID: 30917305] (IHC, Mouse)
• H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis Nat Commun, 2019-01-14;10(1):181. 2019-01-14 [PMID: 30643142] (IHC, Mouse)
• I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors Mol Pain, 2018-11-02;14(0):1744806918814. 2018-11-02 [PMID: 30387376] (IHC-Fr, Mouse)
• Sampurna Chakrabarti, Luke A. Pattison, Kaajal Singhal, James R.F. Hockley, Gerard Callejo, Ewan St. John Smith Acute inflammation sensitizes knee-innervating sensory neurons and decreases mouse digging behavior in a TRPV1-dependent manner Neuropharmacology 12/1/2018 [PMID: 30240782]
• R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats eNeuro, 2018-02-15;5(1):. 2018-02-15 [PMID: 29468190] (IHC, Rat)
• SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur Neuroscience, 2018-02-10;0(0):. 2018-02-10 [PMID: 29432884] (IHC, Mouse)
• Mikhail Stepanichev, Mikhail Onufriev, Viktor Aniol, Sofia Freiman, Hemma Brandstaetter, Stefan Winter et al. Effects of cerebrolysin on nerve growth factor system in the aging rat brain Restorative Neurology and Neuroscience 11/25/2017 [PMID: 29172008]
• SR Chartier, SA Mitchell, LA Majuta, P Mantyth [EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur Mol Pain, 2017-11-22;0(0):1744806917745. 2017-11-22 [PMID: 29166838] (IHC-Fr, Mouse)
• Sara Nencini, Mitchell Ringuet, Dong-Hyun Kim, Yu-Jen Chen, Claire Greenhill, Jason J Ivanusic Mechanisms of nerve growth factor signaling in bone nociceptors and in an animal model of inflammatory bone pain Mol Pain 3/23/2017 [PMID: 28326938]
• EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway J. Neurosci. Methods, 2017-03-07;282(0):34-42. 2017-03-07 [PMID: 28279735] (ELISA Development (Capture), Rat)
• Jorge Rubén Cabrera, Abel Viejo-Borbolla, Antonio Alcamí, Francisco Wandosell Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1 Journal of Neuroinflammation 12/1/2016 [PMID: 27576911]
• Jean-Marc G. Guedon, Geraldine Longo, Lisa A. Majuta, Michelle L. Thomspon, Michelle N. Fealk, Patrick W. Mantyh Dissociation between the relief of skeletal pain behaviors and skin hypersensitivity in a model of bone cancer pain Pain 6/30/2016 [PMID: 27186713]
• Szilard Sajgo, Seid Ali, Octavian Popescu, Tudor Constantin Badea Dynamic expression of transcription factor Brn3b during mouse cranial nerve development Journal of Comparative Neurology 4/1/2016 [PMID: 26356988]
• Szilard Sajgo, Seid Ali, Octavian Popescu, Tudor Constantin Badea Dynamic expression of transcription factor Brn3b during mouse cranial nerve development Journal of Comparative Neurology 2016-04-01 [PMID: 26356988] (Immunohistochemistry, Mouse)
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse)
• Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain. J Neurosci, 2014-01-22;34(4):1494-509. 2014-01-22 [PMID: 24453337] (IHC, Rat)
• Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain. BMC Neurosci, 2014-01-01;15(0):1. 2014-01-01 [PMID: 24380503] (IHC-Fr, Rat)
• Saïda Hadjab, Marina C. M. Franck, Yiqiao Wang, Ulrich Sterzenbach, Anil Sharma, Patrik Ernfors et al. A Local Source of FGF Initiates Development of the Unmyelinated Lineage of Sensory Neurons The Journal of Neuroscience 11/6/2013 [PMID: 24198358]
• Matusica , Dusan, Skeldal , Sune, Sykes , Alex M, Palstra , Nickless, Sharma , Aanchal, Coulson , Elizabet An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem, 2013-03-07;288(16):11144-54. 2013-03-07 [PMID: 23471969] (Flow Cytometry, Human, Rat)
• Chih H. King, Steven S. Scherer Kv7.5 is the primary Kv7 subunit expressed in C-fibers The Journal of Comparative Neurology 6/15/2012 [PMID: 22134895]
• Juan Miguel Jimenez-Andrade, Joseph R. Ghilardi, Gabriela Castañeda-Corral, Michael A. Kuskowski, Patrick W. Mantyh Preventive or late administration of anti-NGF therapy attenuates tumor-induced nerve sprouting, neuroma formation, and cancer pain Pain 11/1/2011 [PMID: 21907491]
• Aaron P. Bloom, Juan M. Jimenez-Andrade, Reid N. Taylor, Gabriela Castañeda-Corral, Magdalena J. Kaczmarska, Katie T. Freeman et al. Breast Cancer-Induced Bone Remodeling, Skeletal Pain, and Sprouting of Sensory Nerve Fibers The Journal of Pain 2011-06-01 [PMID: 21497141] (Immunohistochemistry, Xenograft)
• Aaron P. Bloom, Juan M. Jimenez-Andrade, Reid N. Taylor, Gabriela Castañeda-Corral, Magdalena J. Kaczmarska, Katie T. Freeman et al. Breast Cancer-Induced Bone Remodeling, Skeletal Pain, and Sprouting of Sensory Nerve Fibers The Journal of Pain 6/1/2011 [PMID: 21497141]
• Gabriela Castañeda-Corral, Juan M. Jimenez-Andrade, Aaron P. Bloom, Reid N. Taylor, William G. Mantyh, Magdalena J. Kaczmarska et al. The majority of myelinated and unmyelinated sensory nerve fibers that innervate bone express the tropomyosin receptor kinase A Neuroscience 3/31/2011 [PMID: 21277945]
• Gabriela Castañeda-Corral, Juan M. Jimenez-Andrade, Aaron P. Bloom, Reid N. Taylor, William G. Mantyh, Magdalena J. Kaczmarska et al. The majority of myelinated and unmyelinated sensory nerve fibers that innervate bone express the tropomyosin receptor kinase A Neuroscience 2011-03-31 [PMID: 21277945] (Immunohistochemistry, Mouse)
• Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87. 2010-02-02 [PMID: 20138055] (Neutralization, Rat)

FAQs for TrkA Antibody (AF1056). (Showing 1 - 1 of 1 FAQs).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: NTRK1
• Uniprot:
  • P35739()