TrkA Antibody [Unconjugated]

Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TrkA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat TrkA
  Ala33-Pro418
  Accession # P35739
• Specificity: Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTRK1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkA Antibody [Unconjugated]

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
2. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkA Antibody (AF1056)(25)

Publications using AF1056

• N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy Cell Reports, 2023-03-21;42(4):112295. 2023-03-21 [PMID: 36947543] (IHC, Mouse)
• DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain Oncogene, 2022-06-02;12(1):9241. 2022-06-02 [PMID: 35655086] (IHC, Human)
• K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch Nature Communications, 2022-05-02;13(1):2367. 2022-05-02 [PMID: 35501343] (IHC, Mouse)
• S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons Frontiers in Physiology, 2021-07-15;12(0):644929. 2021-07-15 [PMID: 34335288] (IHC, Rat)
• K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021-04-07;146(3):125-135. 2021-04-07 [PMID: 34030795] (IHC, Rat)
• MA Landy, M Goyal, KM Casey, C Liu, HC Lai Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation Cell Reports, 2021-03-30;34(13):108913. 2021-03-30 [PMID: 33789102] (IHC, Mouse)
• M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone Molecular pain, 2020-01-01;16(0):1744806920975. 2020-01-01 [PMID: 33280501] (Western Blot, Rat)
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020-11-24;33(8):108425. 2020-11-24 [PMID: 33238113] (IHC, Rat)
• L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020-08-21;11(1):4175. 2020-08-21 [PMID: 32826903] (IHC, Mouse)
• M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain J Neuroinflammation, 2020-06-12;17(1):183. 2020-06-12 [PMID: 32532285] (IHC, Rat)
• Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019-09-12;10(1):4137. 2019-09-12 [PMID: 31515492] (ICC, Mouse)
• X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity Cell Rep, 2019-06-25;27(13):3799-3807.e3. 2019-06-25 [PMID: 31242414] (IHC, Mouse)
• S Wiszniak, Q Schwarz Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration BMC Neurosci, 2019-04-29;20(1):21. 2019-04-29 [PMID: 31036074] (IHC-F, Mouse)
• L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Cell Rep, 2019-03-26;26(13):3484-3492.e4. 2019-03-26 [PMID: 30917305] (IHC, Mouse)
• H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis Nat Commun, 2019-01-14;10(1):181. 2019-01-14 [PMID: 30643142] (IHC, Mouse)
• I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors Mol Pain, 2018-11-02;14(0):1744806918814. 2018-11-02 [PMID: 30387376] (IHC-Fr, Mouse)
• R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats eNeuro, 2018-02-15;5(1):. 2018-02-15 [PMID: 29468190] (IHC, Rat)
• SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur Neuroscience, 2018-02-10;0(0):. 2018-02-10 [PMID: 29432884] (IHC, Mouse)
• SR Chartier, SA Mitchell, LA Majuta, P Mantyth [EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur Mol Pain, 2017-11-22;0(0):1744806917745. 2017-11-22 [PMID: 29166838] (IHC-Fr, Mouse)
• EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway J. Neurosci. Methods, 2017-03-07;282(0):34-42. 2017-03-07 [PMID: 28279735] (ELISA Development (Capture), Rat)
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse)
• Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain. J Neurosci, 2014-01-22;34(4):1494-509. 2014-01-22 [PMID: 24453337] (IHC, Rat)
• Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain. BMC Neurosci, 2014-01-01;15(0):1. 2014-01-01 [PMID: 24380503] (IHC-Fr, Rat)
• Matusica , Dusan, Skeldal , Sune, Sykes , Alex M, Palstra , Nickless, Sharma , Aanchal, Coulson , Elizabet An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem, 2013-03-07;288(16):11144-54. 2013-03-07 [PMID: 23471969] (Flow Cytometry, Human, Rat)
• Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87. 2010-02-02 [PMID: 20138055] (Neutralization, Rat)

FAQs for TrkA Antibody (AF1056). (Showing 1 - 1 of 1 FAQs).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: NTRK1
• Uniprot:
  • P35739()