TrkA Antibody [Unconjugated]

Images

 
Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG ...read more
TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TrkA Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TrkA
Ala33-Pro418
Accession # P35739
Specificity
Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NTRK1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1056 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkA Antibody [Unconjugated]

  • DKFZp781I14186
  • EC 2.7.10
  • EC 2.7.10.1
  • MTChigh affinity nerve growth factor receptor
  • Neurotrophic tyrosine kinase receptor type 1
  • neurotrophic tyrosine kinase, receptor, type 1
  • NTRK1
  • NTRK-1
  • p140-TrkA
  • TRK1-transforming tyrosine kinase protein
  • TrkA
  • Trk-A
  • TRKAOncogene TRK
  • TRKTRK1
  • tyrosine kinase receptor A

Background

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for TrkA Antibody (AF1056)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ELISA Development (Capture), Flow Cytometry, ICC, IHC, IHC-F, IHC-Fr, Neutralization, Western Blot.


Filter By Application
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(14)
IHC-F
(1)
IHC-Fr
(3)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(12)
Rat
(10)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF1056 Applications Species
S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons Frontiers in Physiology, 2021;12(0):644929. 2021 [PMID: 34335288] (IHC, Rat) IHC Rat
K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021;146(3):125-135. 2021 [PMID: 34030795] (IHC, Rat) IHC Rat
MA Landy, M Goyal, KM Casey, C Liu, HC Lai Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation Cell Reports, 2021;34(13):108913. 2021 [PMID: 33789102] (IHC, Mouse) IHC Mouse
M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone Molecular pain, 2020;16(0):1744806920975. 2020 [PMID: 33280501] (Western Blot, Rat) Western Blot Rat
RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020;33(8):108425. 2020 [PMID: 33238113] (IHC, Rat) IHC Rat
L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020;11(1):4175. 2020 [PMID: 32826903] (IHC, Mouse) IHC Mouse
L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates Nat Commun, 2020;11(1):4175. 2020 [PMID: 32826903] (IHC, Mouse) IHC Mouse
M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain J Neuroinflammation, 2020;17(1):183. 2020 [PMID: 32532285] (IHC, Rat) IHC Rat
Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019;10(1):4137. 2019 [PMID: 31515492] (ICC, Mouse) ICC Mouse
X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity Cell Rep, 2019;27(13):3799-3807.e3. 2019 [PMID: 31242414] (IHC, Mouse) IHC Mouse
Show All 23 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for TrkA Antibody (AF1056). (Showing 1 - 1 of 1 FAQs).

  1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
    • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

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Blogs on TrkA.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol NTRK1
Uniprot