Human TNF RII/TNFRSF1B Quantikine ELISA Kit

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Reactivity HuSpecies Glossary
Applications ELISA

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Human TNF RII/TNFRSF1B Quantikine ELISA Kit Summary

The Quantikine Human sTNF RII Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human sTNF RII in cell culture supernates, serum, plasma, or urine. It contains E. coli-expressed recombinant human sTNF RII as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occu...rring human soluble Type II receptor for TNF, minus 53 amino acids from the proline-rich region of the protein just exterior to the transmembrane domain, with an apparent molecular weight of approximately 19.8 kDa. This immunoassay has been shown to accurately quantitate the recombinant soluble sTNF RII. Results obtained using natural sTNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural sTNF RII. Since the measurement of human sTNF RII by this immunoassay is insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples, (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).
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Natural and recombinant human sTNF RII
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DRT200.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF RII/TNFRSF1B Quantikine ELISA Kit

  • CD120b antigen
  • CD120b
  • Etanercept
  • p75 TNF receptor
  • p75TBPII
  • p75TNFR
  • soluble TNFR1B variant 1
  • TNFBRp80 TNF-alpha receptor
  • TNF-R2
  • TNF-R75
  • TNFR80
  • TNF-R-II
  • tumor necrosis factor beta receptor
  • tumor necrosis factor binding protein 2
  • Tumor necrosis factor receptor 2
  • tumor necrosis factor receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1B
  • Tumor necrosis factor receptor type II


TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for TNF RII/TNFRSF1B (DRT200)(73)

We have publications tested in 7 confirmed species: Human, Mouse, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Primate - Papio hamadryas (Hamadyras Baboon),  Human.

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Primate - Cholrocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
Primate - Papio cynocephalus (Yellow Baboon)
Primate - Papio hamadryas (Hamadyras Baboon)
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Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using DRT200 Applications Species
Nakanuma, S;Gabata, R;Okazaki, M;Seki, A;Hosokawa, K;Yokoyama, T;Katano, K;Sugita, H;Tokoro, T;Takada, S;Makino, I;Taniguchi, T;Harada, K;Yagi, S; Hemophagocytic Lymphohistiocytosis With Elevated Cytokines Related to Macrophage Activation After Liver Transplantation for Autoimmune Hepatitis: A Case Report Transplantation proceedings 2023-08-01 [PMID: 37537076] ( Human)  Human
V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982140] (Human) Human
T Gohda, M Murakoshi, Y Suzuki, M Hiki, T Naito, K Takahashi, Y Tabe Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients PLoS ONE, 2022-10-11;17(10):e0275745. 2022-10-11 [PMID: 36219652] (Human) Human
TH Wu, LH Chang, CH Chu, CM Hwu, HS Chen, LY Lin Soluble tumor necrosis factor receptor 2 is associated with progressive diabetic kidney disease in patients with type 2 diabetes mellitus PLoS ONE, 2022-04-12;17(4):e0266854. 2022-04-12 [PMID: 35413081] (Human) Human
M Manzano, A Talavera-R, E Moreno, N Madrid, MJ Gosalbes, R Ron, F Dronda, JA Pérez-Moli, VF Lanza, J Díaz, S Moreno, B Navia, S Serrano-Vi Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV Nutrients, 2022-03-14;14(6):. 2022-03-14 [PMID: 35334878] (Human) Human
YK Pageot, AL Stanton, PA Ganz, MR Irwin, SW Cole, CM Crespi, EC Breen, KR Kuhlman, JE Bower Socioeconomic Status and Inflammation in Women with Early-stage Breast Cancer: Mediation by Body Mass Index Brain, Behavior, and Immunity, 2021-10-18;99(0):307-316. 2021-10-18 [PMID: 34673177] (Human) Human
M Seifi, N Rastkari, MS Hassanvand, K Naddafi, R Nabizadeh, S Nazmara, H Kashani, A Zare, Z Pourpak, SY Hashemi, M Yunesian Investigating the relationship between particulate matter and inflammatory biomarkers of exhaled breath condensate and blood in healthy young adults Scientific Reports, 2021-06-21;11(1):12922. 2021-06-21 [PMID: 34155256] (Human) Human
MR Dolsen, AG Harvey IL-6, sTNF-R2, and CRP in the context of sleep, circadian preference, and health in adolescents with eveningness chronotype: Cross-sectional and longitudinal treatment effects Psychoneuroendocrinology, 2021-04-26;129(0):105241. 2021-04-26 [PMID: 33932814] (Human) Human
L Ntari, C Nikolaou, K Kranidioti, D Papadopoul, E Christodou, P Chouvardas, F Meier, C Geka, MC Denis, N Karagianni, G Kollias Combination of subtherapeutic anti-TNF dose with dasatinib restores clinical and molecular arthritogenic profiles better than standard anti-TNF treatment Journal of Translational Medicine, 2021-04-23;19(1):165. 2021-04-23 [PMID: 33892739] (Mouse) Mouse
M Oshima, A Hara, T Toyama, M Jun, C Pollock, M Jardine, S Harrap, N Poulter, ME Cooper, M Woodward, J Chalmers, V Perkovic, MG Wong, T Wada Comparison of Circulating Biomarkers in Predicting Diabetic Kidney Disease Progression With Autoantibodies to Erythropoietin Receptor Kidney international reports, 2020-11-10;6(2):284-295. 2020-11-10 [PMID: 33615053] (Human) Human
Show All 73 Publications.

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Gene Symbol TNFRSF1B