IL-2 R beta Antibody (27302)

Images

 
IL‑2 R beta was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB224) overnight at ...read more
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (30 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
27302
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IL-2 R beta Antibody (27302) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869
Specificity
Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rh gamma c, rhIL‑4 R, or rhIL‑6 R is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL2RB
Purity
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Neutralization 0.05-0.15 ug/mL
Publications
Read Publications using
MAB224 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 R beta Antibody (27302)

  • CD122 antigen
  • CD122
  • high affinity IL-2 receptor beta subunit
  • High affinity IL-2 receptor subunit beta
  • IL-15 R beta
  • IL-2 R beta
  • IL-2 receptor subunit beta
  • IL2R beta
  • IL-2R subunit beta
  • IL2RB
  • IL-2Rb
  • interleukin 15 receptor, beta
  • interleukin 2 receptor, beta
  • interleukin-2 receptor subunit beta
  • P70-75
  • p75

Background

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding, and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I membrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. Soluble IL-2 R beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, soluble IL-2 R beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB367
Species: Hu, Ca
Applications: WB, IHC, ICC
AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00005034-B01P
Species: Hu
Applications: WB, ICC/IF
MAB7745
Species: Hu
Applications: WB, Simple Western, IHC, KO
NBP2-67329
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67375
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP1-83227
Species: Hu
Applications: IHC, IHC-P
H00004354-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-87801
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NLS938
Species: Hu
Applications: IHC, IHC-P

Publications for IL-2 R beta Antibody (MAB224)(5)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: ELISA Development, Flow Cytometry, Neutralization.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(3)
Neutralization
(1)
All Applications
Filter By Species
Human
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB224 Applications Species
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016;6(0):32320. 2016 [PMID: 27586092] (Flow Cytometry, Human) Flow Cytometry Human
Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Tasken K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties. Eur. J. Immunol., 2010;40(1):134-41. 2010 [PMID: 19877008] (Flow Cytometry, Human) Flow Cytometry Human
Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009;182(12):7389-97. 2009 [PMID: 19494261] (ELISA Development, Human) ELISA Development Human
Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection. J. Immunol., 2009;182(3):1439-48. 2009 [PMID: 19155491] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque)) Flow Cytometry Primate - Macaca mulatta (Rhesus Macaque)
Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 2004;173(2):1463-76. 2004 [PMID: 15240743] (Neutralization, Human) Neutralization Human

Reviews for IL-2 R beta Antibody (MAB224) (0)

There are no reviews for IL-2 R beta Antibody (MAB224). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-2 R beta Antibody (MAB224) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-2 R beta Products

Bioinformatics Tool for IL-2 R beta Antibody (MAB224)

Discover related pathways, diseases and genes to IL-2 R beta Antibody (MAB224). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-2 R beta Antibody (MAB224)

Discover more about diseases related to IL-2 R beta Antibody (MAB224).
 

Pathways for IL-2 R beta Antibody (MAB224)

View related products by pathway.

PTMs for IL-2 R beta Antibody (MAB224)

Learn more about PTMs related to IL-2 R beta Antibody (MAB224).

Blogs on IL-2 R beta

There are no specific blogs for IL-2 R beta, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-2 R beta Antibody (27302) and receive a gift card or discount.

Bioinformatics

Gene Symbol IL2RB
Uniprot