KHSRP Antibody

Images

 
Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunohistochemistry: KHSRP Antibody [NBP1-18910] - Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained for GS ...read more
Independent Antibodies: Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
Immunohistochemistry-Frozen: KHSRP Antibody [NBP1-18910] - KSRP immunostaining in mouse cerebral cortex and hippocampus. Cell were stained with Novus KSRP. This KSRP antibody was also used for immunoprecipation and ...read more
Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiKSRP ...read more
Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

KHSRP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human KH-type splicing regulatory protein using the numbering given in entry Q92945.3 (GeneID 8570).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KHSRP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Simple Western 1:40
  • Western Blot 1:2000-1:10000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. IHC-Frozen per customer review. ICC/IF reactivity reported in (PMID: 25907681).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-18910 in the following applications:

Publications
Read Publications using
NBP1-18910 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    2 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25907681).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KHSRP Antibody

  • FBP2far upstream element-binding protein 2
  • FUBP2p75
  • FUSE binding protein 2
  • FUSE-binding protein 2
  • KH type-splicing regulatory protein
  • KH-type splicing regulatory protein
  • KSRPMGC99676

Background

KH-type splicing regulatory protein (KSRP) contains four K homology RNA-binding domains. KH domains are found in a wide variety of proteins including ribosomal proteins, transcription factors, and mRNA processing proteins. KSRP has been found to promote the degradation of mRNAs that encode proteins involved in proliferation and the inflammatory response. Alternative names for KSRP include Far upstream element-binding protein 2, FUSE-binding protein 2, p75 and FUBP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for KHSRP Antibody (NBP1-18910)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(1)
IP
(2)
WB
(2)
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Human
(2)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-18910 Applications Species
Schmidtke L, Meineck M, Saurin S Et al. Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Faslpr Mice Cells Nov 14 2021 [PMID: 34831390] (WB, Mouse) WB Mouse
Schmidtke L, Schrick K, Saurin S et al. The KH-type splicing regulatory protein (KSRP) regulates type III interferon expression post-transcriptionally Biochem. J. Dec 21 2018 [PMID: 30578289] (IP, Human) IP Human
Agca C, Boldt K, Gubler A et al. Expression of leukemia inhibitory factor in Muller glia cells is regulated by a redox-dependent mRNA stability mechanism BMC Biol. 2015 Apr 25 [PMID: 25907681] (ICC/IF, Rat) ICC/IF Rat
Bird CW, Gardiner AS, Bolognani F et al. KSRP Modulation of GAP-43 mRNA Stability Restricts Axonal Outgrowth in Embryonic Hippocampal Neurons. PLoS One. 2013 Nov 14 [PMID: 24244461] (IP, Mouse) IP Mouse
Zhou R, Gong AY, Eischeid AN et al. miR-27b targets KSRP to coordinate TLR4-mediated epithelial defense against Cryptosporidium parvum infection. PLoS Pathog 2012 [PMID: 22615562]
Neff AT, Lee JY, Wilusz J et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Genome Res 2012 Aug [PMID: 22534399] (WB, Human) WB Human
Otsuka M, Takata A, Yoshikawa T et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One 2011 [PMID: 21935400]

Review for KHSRP Antibody (NBP1-18910) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-18910:
Filter by Applications
Immunohistochemistry-Frozen
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen KHSRP NBP1-18910
Enlarge
5
reviewed by:
Nora Perrone-Bizzozero
Immunohistochemistry-Frozen Mouse 02/24/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse brain
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KHSRP Antibody (NBP1-18910) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Nora Perrone-Bizzozero
02/24/2014
Application: Immunohistochemistry-Frozen
Species: Mouse

Bioinformatics

Gene Symbol KHSRP
Entrez
Uniprot