Recombinant Human NT-3 Protein

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1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human NT-3 (Catalog #267‑N3) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human NT-3 Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.
The specific activity of Recombinant Human NT-3 is approximately 2082 U/μg, which is calibrated against recombinant human NT-3 Standard (NIBSC code: 98/718).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-3 protein
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Gene
NTF3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
267-N3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for NT-3 (267-N3)(43)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Primate, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(34)
Cell Culture
(3)
Differentiation
(2)
ELISA (Standard)
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(25)
Mouse
(9)
Rat
(4)
Canine
(1)
Chicken
(1)
N/A
(1)
Primate
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 267-N3 Applications Species
EE Burke, JG Chenoweth, JH Shin, L Collado-To, SK Kim, N Micali, Y Wang, C Colantuoni, RE Straub, DJ Hoeppner, HY Chen, A Sellers, K Shibbani, GR Hamersky, M Diaz Busta, BN Phan, WS Ulrich, C Valencia, A Jaishankar, AJ Price, A Rajpurohit, SA Semick, RW Bürli, JC Barrow, DJ Hiler, SC Page, K Martinowic, TM Hyde, JE Kleinman, KF Berman, JA Apud, AJ Cross, NJ Brandon, DR Weinberger, BJ Maher, RDG McKay, AE Jaffe Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs Nat Commun, 2020;11(1):462. 2020 [PMID: 31974374] (Cell Culture, Human) Cell Culture Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 2019;13(6):1053-1067. 2019 [PMID: 31708478] (Cell Culture, Mouse) Cell Culture Mouse
CY Lin, M Yoshida, LT Li, A Ikenaka, S Oshima, K Nakagawa, H Sakurai, E Matsui, T Nakahata, MK Saito iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases JCI Insight, 2019;4(18):. 2019 [PMID: 31534050] (Bioassay, Human) Bioassay Human
Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend A cell fitness selection model for neuronal survival during development Nat Commun, 2019;10(1):4137. 2019 [PMID: 31515492] (Cell Culture, Chicken) Cell Culture Chicken
J Zhang, L Wang, H Cao, N Chen, B Yan, X Ao, H Zhao, J Chu, M Huang, Z Zhang Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30672120] (In Vivo, Rat) In Vivo Rat
Z Melamed, J López-Erau, MW Baughn, O Zhang, K Drenner, Y Sun, F Freyermuth, MA McMahon, MS Beccari, JW Artates, T Ohkubo, M Rodriguez, N Lin, D Wu, CF Bennett, F Rigo, S Da Cruz, J Ravits, C Lagier-Tou, DW Cleveland Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration Nat. Neurosci., 2019;22(2):180-190. 2019 [PMID: 30643298] (Bioassay, Human) Bioassay Human
M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman Human ESC-Derived Chimeric Mouse Models of Huntington&#039;s Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30554964] (Bioassay, Human) Bioassay Human
N Tsuburaya, K Homma, T Higuchi, A Balia, H Yamakoshi, N Shibata, S Nakamura, H Nakagawa, SI Ikeda, N Umezawa, N Kato, S Yokoshima, M Shibuya, M Shimonishi, H Kojima, T Okabe, T Nagano, I Naguro, K Imamura, H Inoue, T Fujisawa, H Ichijo A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model Nat Commun, 2018;9(1):2668. 2018 [PMID: 29991716] (Bioassay, Human) Bioassay Human
DR Gomez, LA Byers, M Nilsson, L Diao, J Wang, L Li, P Tong, M Hofstad, B Saigal, I Wistuba, N Kalhor, S Swisher, Y Fan, WK Hong, M Suraokar, C Behrens, C Moran, JV Heymach Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer Oncotarget, 2018;9(18):14268-14284. 2018 [PMID: 29581842] (Bioassay, Human) Bioassay Human
Show All 43 Publications.

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Bioinformatics

Gene Symbol NTF3
Uniprot