LEDGF Antibody (762815)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 762815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LEDGF Antibody (762815) Summary

• Immunogen: E. coli-derived recombinant human LEDGF
  Met1-Lys333
  Accession # O75475
• Specificity: Detects human LEDGF in direct ELISAs and Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSIP1
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LEDGF Antibody (762815)

• CLL-associated antigen KW-7
• Dense fine speckles 70 kDa protein
• DFS 70
• DFS70
• LEDGF
• LEDGFDFS 70
• Lens epithelium-derived growth factor
• MGC74712
• p52
• p75
• PAIP
• PC4 and SFRS1 interacting protein 1
• PC4 and SFRS1 interacting protein 2
• PC4 and SFRS1-interacting protein
• PSIP1
• PSIP2
• transcriptional coactivator p52/p75
• Transcriptional coactivator p75/p52

Background

LEDGF, also known as transcription co-activator p75, is a 75 kDa protein that belongs to the hepatoma-derived growth factor (HDGF) family of nuclear targeted proteins. It is a widely expressed protein that interacts with lentiviral integrase. As a result of alternative splicing, isoforms with deletions, substitutions/insertions have been reported. Human LEDGF shares 91%, 92% and 96% amino acid sequence homology with rat, mouse and feline LEDGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LEDGF Antibody (MAB3468)(1)

Publication using MAB3468

• Y Xu, W Zhao, SD Olson, KS Prabhakara, X Zhou Alternative splicing links histone modifications to stem cell fate decision Genome Biol., 2018-09-14;19(1):133. 2018-09-14 [PMID: 30217220] (Western Blot, Human)

Review for LEDGF Antibody (MAB3468) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24040198
• Species: Mouse

Bioinformatics

• Gene Symbol: PSIP1
• Uniprot:
  • O75475()