Recombinant Human CNTF Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human CNTF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
Source
E. coli-derived human CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Ala2
Structure / Form

This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

Protein/Peptide Type
Recombinant Proteins
Gene
CNTF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23-26 kDa, reducing conditions
Publications
Read Publications using
257-NT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CNTF Protein

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

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Publications for CNTF (257-NT)(27)

We have publications tested in 4 confirmed species: Human, Canine, Chicken, Mousee.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(20)
Cell Culture
(2)
ELISA (Capture)
(1)
In Vivo
(2)
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Human
(19)
Canine
(1)
Chicken
(6)
Mousee
(1)
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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 257-NT Applications Species
M Grosch, S Ittermann, E Rusha, T Greisle, C Ori, DJ Truong, AC O'Neill, A Pertek, GG Westmeyer, M Drukker Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation BMC Biol., 2020;18(1):42. 2020 [PMID: 32321486] (Cell Culture, Human) Cell Culture Human
M Chumarina, K Russ, C Azevedo, A Heuer, M Pihl, A Collin, EÅ Frostner, E Elmer, P Hyttel, G Cappellett, M Zini, S Goldwurm, L Roybon Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene Acta Neuropathol Commun, 2019;7(1):208. 2019 [PMID: 31843010] (Bioassay, Human) Bioassay Human
JC Butts, N Iyer, N White, R Thompson, S Sakiyama-E, TC McDevitt V2a interneuron differentiation from mouse and human pluripotent stem cells Nat Protoc, 2019;0(0):. 2019 [PMID: 31628445] (Bioassay, Human) Bioassay Human
KN Babos, KE Galloway, K Kisler, M Zitting, Y Li, Y Shi, B Quintino, RH Chow, BV Zlokovic, JK Ichida Mitigating Antagonism between Transcription and Proliferation Allows Near-Deterministic Cellular Reprogramming Cell Stem Cell, 2019;25(4):486-500.e9. 2019 [PMID: 31523028] (Cell Culture, Mousee) Cell Culture Mousee
Z Melamed, J López-Erau, MW Baughn, O Zhang, K Drenner, Y Sun, F Freyermuth, MA McMahon, MS Beccari, JW Artates, T Ohkubo, M Rodriguez, N Lin, D Wu, CF Bennett, F Rigo, S Da Cruz, J Ravits, C Lagier-Tou, DW Cleveland Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration Nat. Neurosci., 2019;22(2):180-190. 2019 [PMID: 30643298] (Bioassay, Human) Bioassay Human
JR Klim, LA Williams, F Limone, I Guerra San, BN Davis-Duse, DA Mordes, A Burberry, MJ Steinbaugh, KK Gamage, R Kirchner, R Moccia, SH Cassel, K Chen, BJ Wainger, CJ Woolf, K Eggan ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair Nat. Neurosci., 2019;22(2):167-179. 2019 [PMID: 30643292] (Bioassay, Human) Bioassay Human
N Rodriguez-, A Parkhitko, T Grass, RM Gibbs, EM Norabuena, N Perrimon, R Singh, LL Rubin Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29672276] (Bioassay, Human) Bioassay Human
JY Chang, L Shi, ML Ko, GY Ko Circadian Regulation of Mitochondrial Dynamics in Retinal Photoreceptors J. Biol. Rhythms, 2018;33(2):151-165. 2018 [PMID: 29671706] (Bioassay, Chicken) Bioassay Chicken
MJ Ziller, JA Ortega, KA Quinlan, DP Santos, H Gu, EJ Martin, C Galonska, R Pop, S Maidl, A Di Pardo, M Huang, HY Meltzer, A Gnirke, CJ Heckman, A Meissner, E Kiskinis Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology Cell Stem Cell, 2018;0(0):. 2018 [PMID: 29551301] (Bioassay, Human) Bioassay Human
C Coste, V Neirinckx, A Sharma, G Agirman, B Rogister, J Foguenne, F Lallemend, A Gothot, S Wislet Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues PLoS ONE, 2017;12(7):e0177962. 2017 [PMID: 28683107] (Bioassay, Human) Bioassay Human
Show All 27 Publications.

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Bioinformatics

Gene Symbol CNTF
Uniprot