BDNF Antibody (35928.11) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: BDNF Antibody (35928.11) [NB120-10505] - Human thyroid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: 35928.11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BDNF Antibody (35928.11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse BDNF Antibody (35928.11) - Azide and BSA Free (NB120-10505) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-BDNF Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human BDNF (rhBDNF) expressed in Sf 21 insect cells.
• Localization: Secreted
• Specificity: Monoclonal Anti-Human BDNF will neutralize the biological activity of rhBDNF. This antibody shows < 2% cross-reactivity with rhbeta-NGF, rhNT-3 and rhNT-4 based on ELISA and immunoblotting.
  
  The antibody shows ~5% cross-reactivity with recombinant human beta-NGF and recombinant rat beta-NGF
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BDNF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 8 - 25 ug/mL
  • Simple Western
  • Western Blot 1 - 2 ug/mL
• Application Notes: WB: This antibody detects BDNF at 300 ng/lane under non-reducing and reducing conditions. Immunohistochemistry (formalin-fixed, paraffin-embedded sections): 8-25 ug/mL using human spinal cord. This BDNF Antibody (35928.11) is validated for ICC/IF from a verified customer review.
  
  See Simple Western Antibody Database for Simple Western validation: separated by size
• Control:

  BDNF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 5% trehalose
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for BDNF Antibody (35928.11) - Azide and BSA Free

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (NB120-10505)(6)

Publications using NB120-10505

• Du Y, Gao F, Sun H et al. Novel substituted 4-(Arylethynyl)-Pyrrolo[2,3-d]pyrimidines negative allosteric modulators (NAMs) of the metabotropic glutamate receptor subtype 5 (mGlu5) Treat depressive disorder in mice European journal of medicinal chemistry 2023-12-05 [PMID: 37847955]
• Ji M, Zhang Z, Gao F et al. Curculigoside rescues hippocampal synaptic deficits elicited by PTSD through activating cAMP-PKA signaling Phytotherapy research : PTR 2022-10-06 [PMID: 36200803]
• Elwakil BH, Bakr BA, Aljeldah MM et al. Memory Impairment, Pro-Inflammatory Host Response and Brain Histopathologic Severity in Rats Infected with K. pneumoniae or P. aeruginosa Meningitis Pathogens (Basel, Switzerland) 2022-08-18 [PMID: 36015052] (WB, Rat)
  
  Details:
  WB 1:500
• Bales KL, Chacko AS, Nickerson JM et al. Treadmill exercise promotes retinal astrocyte plasticity and protects against retinal degeneration in a mouse model of light-induced retinal degeneration Journal of Neuroscience Research 2022-09-01 [PMID: 35582827] (Western Blot)
• Wang W, Gu MF, Wang ZF Et al. Let-7a-5p regulated by lncRNA-MEG3 promotes functional differentiation to Schwann cells from adipose derived stem cells via directly inhibiting RBPJ-mediating Notch pathway Apoptosis : an international journal on programmed cell death 2021-08-18 [PMID: 34409556]
• Cho K, Kim Gw Selective striatal cell loss is ameliorated by regulated autophagy of the cortex Life sciences 2021-07-14 [PMID: 34271058] (WB)

Review for BDNF Antibody (NB120-10505) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 03/01/2019

Summary

• Application: Immunofluorescence
• Sample Tested: Thyroid tissue
• Species: Human
• Lot: 04M1323

Comments

• Comments: Human thryoid tissue was incubated with anti-BDNF antibody at a dilution of 1:100 overnight at 4C and detected with Alexa Fluor 594 Donkey anti-mouse 1:500 Abcam, cat# Ab150112. Nuclei stained with DAPI.

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)