Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free

Images

 
Biological Strategies: Western Blot: Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free [NBP2-31099] - LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
156C135.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.
Modification
p Ser213, p Ser210
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-4 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:20-1:1000
Application Notes
Immunohistochemistry-Paraffin reported in literature (Lin et al, 2007).

Reactivity Notes

New World Monkey. Predicted cross-reactivity with Chimpanzee, Dog, Mouse, Pig and Rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

The androgen receptor (AR) is an approx. 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790 (Lin et al. 2003). The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5415
Species: Hu
Applications: WB, IHC, ICC
NB300-560
Species: Hu, Mu, Rt, Av, Bv, Pm
Applications: WB, IHC, IHC-Fr, IHC-P, IF
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IF
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00005555-P01
Species: Hu
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-1596
Species: Hu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC
NB200-305
Species: Hu, Mu, Mk, Rb
Applications: WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-P, IP
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-53144
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ye, Ze
Applications: WB, IHC
NBP2-02164
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00000126-D01P
Species: Hu, Mu
Applications: WB
NBP2-75398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90233
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31099
Species: Hu, Pm
Applications: WB, IHC, IHC-P
Supplier Logo

Publications for Androgen R/NR3C4 Antibody (NBP2-31099) (0)

There are no publications for Androgen R/NR3C4 Antibody (NBP2-31099).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Androgen R/NR3C4 Antibody (NBP2-31099) (0)

There are no reviews for Androgen R/NR3C4 Antibody (NBP2-31099). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Androgen R/NR3C4 Antibody (NBP2-31099) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Androgen R/NR3C4 Products

Bioinformatics Tool for Androgen R/NR3C4 Antibody (NBP2-31099)

Discover related pathways, diseases and genes to Androgen R/NR3C4 Antibody (NBP2-31099). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Androgen R/NR3C4 Antibody (NBP2-31099)

Discover more about diseases related to Androgen R/NR3C4 Antibody (NBP2-31099).
 

Pathways for Androgen R/NR3C4 Antibody (NBP2-31099)

View related products by pathway.

PTMs for Androgen R/NR3C4 Antibody (NBP2-31099)

Learn more about PTMs related to Androgen R/NR3C4 Antibody (NBP2-31099).
 

Research Areas for Androgen R/NR3C4 Antibody (NBP2-31099)

Find related products by research area.

Blogs on Androgen R/NR3C4

There are no specific blogs for Androgen R/NR3C4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free and receive a gift card or discount.

Bioinformatics

Gene Symbol AR
Entrez