ER beta/NR3A2 Antibody (14C8)

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Western Blot: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:5000.
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Human breast carcinoma. Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] diluted at 1:200. Antigen Retrieval: Citrate ...read more
Immunohistochemistry-Paraffin: Estrogen Receptor beta Antibody (14C8) [NB200-305] - Infiltrating lobular carcinoma of the breast.

Product Details

Summary
Reactivity Hu, Mu, Mk, RbSpecies Glossary
Applications WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
14C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

ER beta/NR3A2 Antibody (14C8) Summary

Immunogen
Amino acids 1-153 of human ER-beta expressed in E. coli.
Specificity
Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ESR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Chromatin Immunoprecipitation
  • Dot Blot
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation
Application Notes
IP usage reported in scientific literature. Strong ER beta staining in epithelial cell nuclei. Occassional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also detected ER beta in colon and ovarian tumors.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB200-305 in the following applications:

Reactivity Notes

Rabbit reactivity reported in (PMID: 23664379 ). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for ER beta/NR3A2 Antibody (14C8)

  • ER beta
  • Erb
  • ER-beta
  • ESR2
  • ESR-BETA
  • ESTRB
  • estrogen receptor 2 (ER beta)
  • estrogen receptor beta 4
  • estrogen receptor beta
  • NR3A2
  • NR3A2ESRB
  • Nuclear receptor subfamily 3 group A member 2

Background

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domains have diverged significantly. Similar in function to ER-alpha, ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ER beta/NR3A2 Antibody (NB200-305)(73)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, IHC-P, IP.


Filter By Application
ChIP
(64)
ICC/IF
(1)
IHC
(2)
IHC-P
(3)
IP
(1)
All Applications
Filter By Species
Human
(67)
Mouse
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using NB200-305 Applications Species
Monteiro AC, Muenzner JK, Andrade F et al. Gene expression in oligodendrocytes during remyelination reveals cholesterol homeostasis as a therapeutic target in multiple sclerosis Proc. Natl. Acad. Sci. U.S.A. May 14 2019 [PMID: 31040210] (ChIP, Mouse) ChIP Mouse
Castelan F, Xelhuantzi N, Hernandez-Aragon LG et al. Morphometry of paravaginal ganglia from the pelvic plexus: impact of multiparity, primiparity, and pregnancy. Eur J Obstet Gynecol Reprod Biol 2013 Aug 6 [PMID: 23932302] (IHC-P, Rabbit) IHC-P Rabbit
Jardel P, Debiais C, Godet J, Irani J, Fromont G. Ductal Carcinoma Of The Prostate Shows A Different Immunophenotype From High Grade Acinar Cancer. Histopathology 2013 Mar 11 [PMID: 23701402] (IHC-P, Human) IHC-P Human
Lopez-Garcia K, E Cuevas, Corona-Quintanilla DL et al. Effect of multiparity on morphometry and oestrogen receptor expression of pelvic and perineal striated muscles in rabbits: is serum oestradiol relevant?. Eur J Obstet Gynecol Reprod Bio 2013 May 7 [PMID: 23664379] (IHC-P, Rabbit) IHC-P Rabbit
Kauffman EC, Robinson BD, Downes M et al. Estrogen receptor-beta expression and pharmacological targeting in bladder cancer. Oncol Rep 2013 Jul [PMID: 23612777]
Verma Primate, Miki Y, Abe K et al. Co-expression of estrogen receptor beta and aromatase in Japanese lung cancer patients: gender-dependent clinical outcome. Life Sci 2012 Oct [PMID: 22982181] (ChIP, Human) ChIP Human
Sato R, Suzuki T, Katayose Y et al. Aromatase in colon carcinoma. Anticancer Res 2012 Aug [PMID: 22843875] (ChIP, Human) ChIP Human
Cheng L, Li J, Han Y et al. PES1 promotes breast cancer by differentially regulating ER alpha and ER beta J Clin Invest 2012 Aug 1 [PMID: 22820289] (ChIP, IP, Human) ChIP, IP Human
Karlsson C, Helenius G, Fernandes O et al. Oestrogen receptor B in NSCLC - prevalence, proliferative influence, prognostic impact and smoking. APMIS 2012 Jun [PMID: 22583357] (ChIP, Human) ChIP Human
Tonetti DA, Gao W, Escarzaga D et al. PKC alpha and ERbeta Are Associated with Triple-Negative Breast Cancers in African American and Caucasian Patients Int J Breast Cancer 2012 [PMID: 22500240] (IHC, Human) IHC Human
Show All 73 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ESR2
Entrez
Uniprot