PSAT1 Antibody

Images

 
Western Blot: PSAT1 Antibody [NBP1-32920] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PSAT1 Antibody [NBP1-32920] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PSAT1 Antibody [NBP1-32920] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Genetic Strategies: Knockdown Validated: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

PSAT1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.
Predicted Species
Rat (95%), Bovine (95%), Rabbit (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32920 in the following applications:

  • B/N
    2 publications
  • 1 publication
  • WB
    7 publications

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for PSAT1 Antibody

  • EC 2.6.1.52
  • EPIP
  • Phosphohydroxythreonine aminotransferase
  • phosphoserine aminotransferase 1
  • phosphoserine aminotransferase
  • PSAMGC1460
  • PSAT
  • PSAT1
  • PSATD
  • PSATendometrial progesterone-induced protein

Background

The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSAT1 Antibody (NBP1-32920)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: B/N, IHC-P, WB.


Filter By Application
B/N
(2)
IHC-P
(1)
WB
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-32920 Applications Species
Lee G, Wong C, Cho A et al. Serine synthesis pathway upregulated by E-cadherin is essential for the proliferation and metastasis of breast cancers bioRxiv 2023-05-25 [PMID: 37292712] (WB, Human) WB Human
Wang K, Luo L, Fu S et al. PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma Nature communications 2023-02-23 [PMID: 36823188] (WB) WB
Jiang J, Chen HN, Jin P et al. Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant Signal transduction and targeted therapy 2023-02-15 [PMID: 36788227] (IHC-P, Human) IHC-P Human
Di Marcantonio D, Martinez E, Kanefsky JS et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia Molecular cell 2021-07-01 [PMID: 34081901]
Montrose DC, Saha S, Foronda M et al. Exogenous and Endogenous Sources of Serine Contribute to Colon Cancer Metabolism, Growth, and Resistance to 5-Fluorouracil Cancer Research 2021-05-01 [PMID: 33526512] (B/N, WB) B/N, WB
Jiang J, Zhang L, Chen H et al. Regorafenib induces lethal autophagy arrest by stabilizing PSAT1 in glioblastoma Autophagy 2019-03-25 [PMID: 30909789] (WB, Human) WB Human
Hulea L, Gravel SP, Morita M et al. Translational and HIF-1a-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides. Cell Metab. 2018-09-17 [PMID: 30244971] (B/N) B/N
Toonen Lodewijk J A, Overzier Maurice, Evers Melvin M et al. Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model. Molecular Neurodegeneration 2018-06-22 [PMID: 29929540] (WB, Mouse) WB Mouse
Maddocks ODK, Athineos D, Cheung EC et al. Modulating the therapeutic response of tumours to dietary serine and glycine starvation. Nature. 2017-04-19 [PMID: 28425994] (WB, Mouse) WB Mouse
Samanta D, Park Y, Andrabi SA et al. PHGDH Expression is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance and Lung Metastasis. Cancer Res. 2016-06-08 [PMID: 27280394] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PSAT1