PSAT1 Antibody

Western Blot: PSAT1 Antibody [NBP1-32920] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PSAT1 Antibody [NBP1-32920] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PSAT1 Antibody [NBP1-32920] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3].

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PSAT1 Antibody Summary

• Description: Novus Biologicals Rabbit PSAT1 Antibody (NBP1-32920) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PSAT1 Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.
• Predicted Species: Rat (95%), Bovine (95%), Rabbit (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSAT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PSAT1 Antibody

• EC 2.6.1.52
• EPIP
• Phosphohydroxythreonine aminotransferase
• phosphoserine aminotransferase 1
• phosphoserine aminotransferase
• PSAMGC1460
• PSAT
• PSAT1
• PSATD
• PSATendometrial progesterone-induced protein

Background

The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSAT1 Antibody (NBP1-32920)(13)

Publications using NBP1-32920

• Vasileios Papalazarou, Alice C. Newman, Alejandro Huerta-Uribe, Nathalie M. Legrave, Mattia Falcone, Tong Zhang, Lynn McGarry, Dimitris Athineos, Emma Shanks, Karen Blyth, Karen H. Vousden, Oliver D. K. Maddocks Phenotypic profiling of solute carriers characterizes serine transport in cancer Nature Metabolism 2023-12-08 [PMID: 38066114]
• Kim Y, Ju H, Yoo SY et Al. Glutathione dynamics is a potential predictive and therapeutic trait for neoadjuvant chemotherapy response in bladder cancer Cell Rep Med 2023-10-04 [PMID: 37797616]
• Lee G, Wong C, Cho A et al. Serine synthesis pathway upregulated by E-cadherin is essential for the proliferation and metastasis of breast cancers bioRxiv 2023-05-25 [PMID: 37292712] (WB, Human)
• Wang K, Luo L, Fu S et al. PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma Nature communications 2023-02-23 [PMID: 36823188] (WB)
• Jiang J, Chen HN, Jin P et al. Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant Signal transduction and targeted therapy 2023-02-15 [PMID: 36788227] (IHC-P, Human)
• Jennifer X Ji, Dawn R Cochrane, Gian Luca Negri, Shane Colborne, Sandra E Spencer Miko, Lynn N Hoang, David Farnell, Basile Tessier-Cloutier, Jutta Huvila, Emily Thompson, Samuel Leung, Derek Chiu, Christine Chow, Monica Ta, Martin Köbel, Lucas Feil, Michael Anglesio, Ellen L Goode, Kelly Bolton, Gregg B Morin, David G Huntsman The proteome of clear cell ovarian carcinoma. The Journal of pathology 2022-11-10 [PMID: 36031730]
• Di Marcantonio D, Martinez E, Kanefsky JS et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia Molecular cell 2021-07-01 [PMID: 34081901]
• Montrose DC, Saha S, Foronda M et al. Exogenous and Endogenous Sources of Serine Contribute to Colon Cancer Metabolism, Growth, and Resistance to 5-Fluorouracil Cancer Research 2021-05-01 [PMID: 33526512] (Western Blot, Human)
• Jiang J, Zhang L, Chen H et al. Regorafenib induces lethal autophagy arrest by stabilizing PSAT1 in glioblastoma Autophagy 2019-03-25 [PMID: 30909789] (WB, Human)
• Hulea L, Gravel SP, Morita M et al. Translational and HIF-1a-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides. Cell Metab. 2018-09-17 [PMID: 30244971] (Western Blot, Human)
• Toonen Lodewijk J A, Overzier Maurice, Evers Melvin M et al. Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model. Molecular Neurodegeneration 2018-06-22 [PMID: 29929540] (WB, Mouse)
• Maddocks ODK, Athineos D, Cheung EC et al. Modulating the therapeutic response of tumours to dietary serine and glycine starvation. Nature. 2017-04-19 [PMID: 28425994] (WB, Mouse)
• Samanta D, Park Y, Andrabi SA et al. PHGDH Expression is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance and Lung Metastasis. Cancer Res. 2016-06-08 [PMID: 27280394] (WB, Human)

Bioinformatics

• Gene Symbol: PSAT1