PSAT1 Antibody

Western Blot: PSAT1 Antibody [NBP1-32920] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PSAT1 Antibody [NBP1-32920] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PSAT1 Antibody [NBP1-32920] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Knockdown Validated: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3].

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PSAT1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.
• Predicted Species: Rat (95%), Bovine (95%), Rabbit (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for PSAT1 Antibody

• EC 2.6.1.52
• EPIP
• Phosphohydroxythreonine aminotransferase
• phosphoserine aminotransferase 1
• phosphoserine aminotransferase
• PSAMGC1460
• PSAT
• PSAT1
• PSATD
• PSATendometrial progesterone-induced protein

Background

The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSAT1 Antibody (NBP1-32920)(7)

Publications using NBP1-32920

• Wang K, Luo L, Fu S et al. PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma Nature communications 2023-02-23 [PMID: 36823188] (WB)
• Jiang J, Chen HN, Jin P et al. Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant Signal transduction and targeted therapy 2023-02-15 [PMID: 36788227] (IHC-P, Human)
• Di Marcantonio D, Martinez E, Kanefsky JS et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia Molecular cell 2021-07-01 [PMID: 34081901]
• Jiang J, Zhang L, Chen H et al. Regorafenib induces lethal autophagy arrest by stabilizing PSAT1 in glioblastoma Autophagy 2019-03-25 [PMID: 30909789] (WB, Human)
• Toonen Lodewijk J A, Overzier Maurice, Evers Melvin M et al. Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model. Molecular Neurodegeneration 2018-06-22 [PMID: 29929540] (WB, Mouse)
• Maddocks ODK, Athineos D, Cheung EC et al. Modulating the therapeutic response of tumours to dietary serine and glycine starvation. Nature. 2017-04-19 [PMID: 28425994] (WB, Mouse)
• Samanta D, Park Y, Andrabi SA et al. PHGDH Expression is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance and Lung Metastasis. Cancer Res. 2016-06-08 [PMID: 27280394] (WB, Human)

Bioinformatics

• Gene Symbol: PSAT1