Aromatase Antibody

Images

 
Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.
Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596] - Aromatase antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more

Product Details

Summary
Reactivity Hu, Pm, Rb, Bv, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Aromatase Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
Localization
Membrane-bound
Predicted Species
Bovine (90%), Equine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP19A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
Application Notes
This Aromatase antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is seen at ~55 kDa in human brain, and Immunocytochemistry/Immunofluorescence where membrane staining is observed in SH-SY-5Y cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.Use in Immunohistochemistry reported in scientific literature (PMID 28133606).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Temporal Lobe Lysate (NB820-59476)
Aromatase Lysate (NBL1-09674)
Control Peptide
Aromatase Peptide (NB100-1596PEP)
Publications
Read Publications using
NB100-1596 in the following applications:

  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Human and Primate.Rabbit reactivity reported in scientific literature (PMID: 25850953) Immunogen has 91% homology to horse, 90% homology to cow and rabbit, 83% homology to pig, and 81% homology to sheep, goat, and dog proteins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aromatase Antibody

  • ARO1EC 1.14.14.1
  • Aromatase
  • AROsubfamily XIX (aromatization of androgens)
  • CYARMGC104309
  • CYPXIX
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • Cytochrome P-450AROM
  • Estrogen synthase
  • microsomal monooxygenase

Background

Aromatase (ARO1) or cytochrome P450 19A1 (CYP19A1) belongs to cytochrome P450 family of enzymes, which are monooxygenases known for their essential role in drug catabolism and cholesterol/steroid synthesis. Employing heme-group act as a co-factor, ARO1 catalyzes aromatic C18 estrogens formation from C19 androgens and is responsible for conversion of testosterone to estradiol and androstenedione to estrone. ARO1 is localized to cellular membranes as a peripheral membrane protein and in addition to female reproductive tract and adipose tissue, aromatase can be found in testis, liver, brain as well as certain tumors including breast cancer. During ontogenesis, it plays an important role in the development of CNS and gonads as well as sex differentiation. Defective ARO1 may leads to aromatase excess syndrome (AEXS) and aromatase deficiency (AROD). Moreover, obesity has been linked to abnormally high ARO1 expression in mammary tissues, increased local estrogen production, and susceptibility to breast hyperplasia and cancer. ARO1 is the key enzyme in intratumoral estrogen production in postmenopausal mammary cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aromatase Antibody (NB100-1596)(5)

We have publications tested in 3 confirmed species: Human, Primate, Rabbit.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Primate
(2)
Rabbit
(2)
All Species

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Product General Protocols

View specific protocols for Aromatase Antibody (NB100-1596): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aromatase Antibody (NB100-1596). (Showing 1 - 1 of 1 FAQs).

  1. I have a customer with a question about antibody NB100-1596 anti-aromatase. The customer is questioning why the recommended storage conditions are 4C with no freezing. They are concerned that the antibody will not be stable unless it is frozen.
    • For this product, we found that the antibody was quite unstable after just one freeze thaw cycle, which is why we recommend storage at 4C. In our experience, the antibody performs quite well when stored at 4C; even for long periods of time. I hope this will help your customer. Please let them know that if they freeze the antibody, we will not be able to guarantee function.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1596AF405
Alexa Fluor 488 Labeled NB100-1596AF488
Alexa Fluor 647 Labeled NB100-1596AF647
Alexa Fluor 700 Labeled NB100-1596AF700
Biotin Labeled NB100-1596B
DyLight 405 Labeled NB100-1596V
DyLight 488 Labeled NB100-1596G
DyLight 550 Labeled NB100-1596R
DyLight 650 Labeled NB100-1596C
FITC Labeled NB100-1596F
HRP Labeled NB100-1596H

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Blogs on Aromatase.

Aromatase - A Key Enzyme in the Biosynthesis of Estrogens
The enzyme, aromatase, belongs to cytochrome P450 family of monooxygenases known for their key role in drug catabolism and cholesterol/steroid synthesis. Aromatase uses a heme-group as a co-factor to catalyze the formation of aromatic C18 estrogens fr...  Read full blog post.

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Bioinformatics

Gene Symbol CYP19A1