Aromatase Antibody


Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot more
Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were more
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of more
Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was more
Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive more

Product Details

Reactivity Hu, Mu, Rt, Pm, Rb, Bv, EqSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
1.0 mg/ml

Aromatase Antibody Summary

A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
Predicted Species
Bovine (90%), Equine (91%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot 1 ug/ml
Application Notes
This Aromatase antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot, a band is seen at ~55 kDa in human brain, and Immunocytochemistry/Immunofluorescence where membrane staining is observed in SH-SY-5Y cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 28133606). Use in Simple Western reported in scientific literature (PMID:29898754).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aromatase Overexpression Lysate
Control Peptide
Aromatase Peptide (NB100-1596PEP)
Read Publications using
NB100-1596 in the following applications:

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35245530).Rabbit reactivity reported in scientific literature (PMID: 25850953). Immunogen displays the following percentage of sequence identity for non-tested species: horse (91%), cow (90%), rabbit (90%), pig (83%), sheep (81%), goat (81%), and dog (81%) proteins. Rat reactivity reported in scientific literature (PMID: 29898754). Use in Mouse reported in scientific literature (PMID:32634520).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Aromatase Antibody

  • ARO1
  • ARO1EC
  • Aromatase
  • AROsubfamily XIX (aromatization of androgens)
  • CYAR
  • CYARMGC104309
  • CYP19
  • CYP19A1
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • Cytochrome P-450AROM
  • EC
  • Estrogen synthase
  • microsomal monooxygenase


Aromatase (ARO1) or cytochrome P450 19A1 (CYP19A1) belongs to cytochrome P450 family of enzymes, which are monooxygenases known for their essential role in drug catabolism and cholesterol/steroid synthesis. Employing heme-group act as a co-factor, ARO1 catalyzes aromatic C18 estrogens formation from C19 androgens and is responsible for conversion of testosterone to estradiol and androstenedione to estrone. ARO1 is localized to cellular membranes as a peripheral membrane protein and in addition to female reproductive tract and adipose tissue, aromatase can be found in testis, liver, brain as well as certain tumors including breast cancer. During ontogenesis, it plays an important role in the development of CNS and gonads as well as sex differentiation. Defective ARO1 may leads to aromatase excess syndrome (AEXS) and aromatase deficiency (AROD). Moreover, obesity has been linked to abnormally high ARO1 expression in mammary tissues, increased local estrogen production, and susceptibility to breast hyperplasia and cancer. ARO1 is the key enzyme in intratumoral estrogen production in postmenopausal mammary cancer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
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Applications: ICC/IF, IHC, IHC-P, WB
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Applications: IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Hu
Applications: Flow, IHC, IHC-P, WB
Species: Hu, Pm, Mu, Rb
Applications: ChIP, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, RM
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Hu
Applications: IHC, IHC-P, WB

Publications for Aromatase Antibody (NB100-1596)(13)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate, Rabbit.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, Simple Western, WB.

Filter By Application
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-1596 Applications Species
Briski KP, Mahmood ASMH, Uddin MM et al. Effects of Ventromedial Hypothalamic Nucleus (VMN) Aromatase Gene Knockdown on VMN Glycogen Metabolism and Glucoregulatory Neurotransmission Biology 2023-02-03 [PMID: 36829519] (WB, Rat) WB Rat
Wang H, Feng X, Wang T et al. Role and mechanism of the p-JAK2/p-STAT3 signaling pathway in follicular development in PCOS rats General and comparative endocrinology 2022-10-03 [PMID: 36202220] (WB, Rat) WB Rat
Kata D, GrOf I, Hoyk Z et al. Immunofluorescent Evidence for Nuclear Localization of Aromatase in Astrocytes in the Rat Central Nervous System International journal of molecular sciences 2022-08-11 [PMID: 36012212] (IHC-P, IHC-Fr, ICC/IF, Rat, Human)

Dilution used 1:100
IHC-P, IHC-Fr, ICC/IF Rat, Human
Ganesan S, Keating AF Ovarian mitochondrial and oxidative stress proteins are altered by glyphosate exposure in mice Toxicol. Appl. Pharmacol. 2020-07-04 [PMID: 32634520] (WB, Mouse) WB Mouse
Ganesan S, McGuire BC, Keating AF Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis Reprod. Toxicol. 2020-06-24 [PMID: 32592754] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Giles ED, Jindal S, Wellberg EA et al. Metformin inhibits stromal aromatase expression and tumor progression in a rodent model of postmenopausal breast cancer Breast Cancer Res. 2018-06-14 [PMID: 29898754] (Simple Western, Rat) Simple Western Rat
Rodriguez-Castelan J, Mendez-Tepepa M, Carrillo-Portillo Y et al. Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits Biomed Res Int 2017-01-30 [PMID: 28133606] (IF/IHC, WB, Rabbit) IF/IHC, WB Rabbit
de Los Angeles Carrasco-Ruiz M, Garcia-Villamar V, Lopez-Garcia K et al. Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits Cell Biochem. Funct. 2015-04-07 [PMID: 25850953] (WB, Rabbit) WB Rabbit
Pignatti E, Casarini L, Scaltriti S et al. Aromatase expression in human peripheral blood leucocytes (PBLs) and in various tissues in primates: studies in elderly humans and cynomolgus monkeys J Med Primatol 2012-09-17 [PMID: 22978812] (WB, Primate, Human) WB Primate, Human
Bogan RL, Murphy MJ, Hennebold JD. Dynamic changes in gene expression that occur during the period of spontaneous functional regression in the rhesus macaque corpus luteum. Endocrinology;150(3):1521-9. 2009-03-01 [PMID: 18948396]
Show All 13 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aromatase Antibody (NB100-1596). (Showing 1 - 1 of 1 FAQs).

  1. I have a customer with a question about antibody NB100-1596 anti-aromatase. The customer is questioning why the recommended storage conditions are 4C with no freezing. They are concerned that the antibody will not be stable unless it is frozen.
    • For this product, we found that the antibody was quite unstable after just one freeze thaw cycle, which is why we recommend storage at 4C. In our experience, the antibody performs quite well when stored at 4C; even for long periods of time. I hope this will help your customer. Please let them know that if they freeze the antibody, we will not be able to guarantee function.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Aromatase.

Aromatase - A Key Enzyme in the Biosynthesis of Estrogens
The enzyme, aromatase, belongs to cytochrome P450 family of monooxygenases known for their key role in drug catabolism and cholesterol/steroid synthesis. Aromatase uses a heme-group as a co-factor to catalyze the formation of aromatic C18 estrogens fr...  Read full blog post.

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Gene Symbol CYP19A1