Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.
Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596] - Aromatase antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
This Aromatase antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot, a band is seen at ~55 kDa in human brain, and Immunocytochemistry/Immunofluorescence where membrane staining is observed in SH-SY-5Y cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 28133606). Use in Simple Western reported in scientific literature (PMID: 29898754).
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rabbit reactivity reported in scientific literature (PMID: 25850953). Immunogen has 91% homology to horse, 90% homology to cow and rabbit, 83% homology to pig, and 81% homology to sheep, goat, and dog proteins. Rat reactivity reported in scientific literature (PMID: 29898754).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for Aromatase Antibody
AROsubfamily XIX (aromatization of androgens)
cytochrome P450, family 19, subfamily A, polypeptide 1
Aromatase (ARO1) or cytochrome P450 19A1 (CYP19A1) belongs to cytochrome P450 family of enzymes, which are monooxygenases known for their essential role in drug catabolism and cholesterol/steroid synthesis. Employing heme-group act as a co-factor, ARO1 catalyzes aromatic C18 estrogens formation from C19 androgens and is responsible for conversion of testosterone to estradiol and androstenedione to estrone. ARO1 is localized to cellular membranes as a peripheral membrane protein and in addition to female reproductive tract and adipose tissue, aromatase can be found in testis, liver, brain as well as certain tumors including breast cancer. During ontogenesis, it plays an important role in the development of CNS and gonads as well as sex differentiation. Defective ARO1 may leads to aromatase excess syndrome (AEXS) and aromatase deficiency (AROD). Moreover, obesity has been linked to abnormally high ARO1 expression in mammary tissues, increased local estrogen production, and susceptibility to breast hyperplasia and cancer. ARO1 is the key enzyme in intratumoral estrogen production in postmenopausal mammary cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Aromatase Antibody (NB100-1596). (Showing 1 - 1 of 1 FAQs).
I have a customer with a question about antibody NB100-1596 anti-aromatase. The customer is questioning why the recommended storage conditions are 4C with no freezing. They are concerned that the antibody will not be stable unless it is frozen.
For this product, we found that the antibody was quite unstable after just one freeze thaw cycle, which is why we recommend storage at 4C. In our experience, the antibody performs quite well when stored at 4C; even for long periods of time. I hope this will help your customer. Please let them know that if they freeze the antibody, we will not be able to guarantee function.
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