Protein S/PROS1 Antibody [Unconjugated]

Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Protein S/PROS1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protein S
  Ala42-Trp670
  Accession # P07225
• Specificity: Detects human PROS1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant mouse Protein S is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROS1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein S/PROS1 Antibody [Unconjugated]

• EC 3.4.21
• PROS
• PROS1
• protein S (alpha)
• Protein S
• protein Sa
• PS21
• PS22
• PS23
• PS24
• PS25
• PSA
• vitamin K-dependent plasma protein S
• vitamin K-dependent protein S

Background

Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42 ‑ 87), four EGF-like repeats (aa 117 - 283) and two laminin G-like domains (aa 299 ‑ 666). Over aa 42 - 670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein S/PROS1 Antibody (AF4036)(3)

Publications using AF4036

• BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 2018;515(0):176-190. 2018 [PMID: 29304470] (Western Blot, Human)
• Zagorska A, Traves P, Lew E, Dransfield I, Lemke G Diversification of TAM receptor tyrosine kinase function. Nat Immunol, 2014;15(10):920-8. 2014 [PMID: 25194421] (Western Blot, Mouse)
• Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010;115(23):4963-72. 2010 [PMID: 20348395] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PROS1
• Uniprot:
  • P07225()