Human Kallikrein 3/PSA Quantikine ELISA Kit

Product Details

Reactivity HuSpecies Glossary
Applications ELISA

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Human Kallikrein 3/PSA Quantikine ELISA Kit Summary

The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quant...ikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.
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Natural and recombinant human KLK3/PSA
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DKK300.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Kallikrein 3/PSA Quantikine ELISA Kit

  • APS seminin
  • EC 3.4.21
  • EC
  • Gamma-seminoprotein
  • hK3
  • Kallikrein 3
  • kallikrein 3, (prostate specific antigen)
  • Kallikrein-3
  • kallikrein-related peptidase 3
  • KLK2A1
  • KLK3
  • P-30 antigen
  • prostate specific antigen
  • prostate-specific antigen
  • PSA semenogelase
  • PSA
  • Semenogelase
  • Seminin


The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.

Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for Kallikrein 3/PSA (DKK300)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using DKK300 Applications Species
RN Cole, W Chen, LE Pascal, JB Nelson, P Wipf, Z Wang (+)-JJ-74-138 is a novel non-competitive androgen receptor antagonist Molecular Cancer Therapeutics, 2022;0(0):. 2022-01-01 [PMID: 35058329] (Human) Human
TC Case, A Merkel, M Ramirez-So, Q Liu, JA Sterling, R Jin Blocking GRP/GRP-R signaling decreases expression of androgen receptor splice variants and inhibits tumor growth in castration-resistant prostate cancer Translational Oncology, 2021;14(11):101213. 2021-01-01 [PMID: 34461557] (Mouse) Mouse
CP Mao, SC Wang, YP Su, SH Tseng, L He, AA Wu, RBS Roden, J Xiao, CF Hung Protein detection in blood with single-molecule imaging Science Advances, 2021;7(33):. 2021-01-01 [PMID: 34380620] (Human) Human
AL D'Souza, JR Chevillet, P Ghanouni, X Yan, M Tewari, SS Gambhir Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence PLoS ONE, 2018;13(3):e0194268. 2018-01-01 [PMID: 29547636] (Human) Human
MI Suominen, KM Fagerlund, JP Rissanen, YM Konkol, JP Morko, Z Peng, EJ Alhoniemi, SK Laine, E Corey, D Mumberg, K Ziegelbaue, SM Käkönen, J Halleen, RL Vessella, A Scholz Radium-223 inhibits osseous prostate cancer growth by dual targeting of cancer cells and bone microenvironment in mouse models Clin. Cancer Res., 2017;0(0):. 2017-01-01 [PMID: 28364014] (Mouse) Mouse
V Sanna, CK Singh, R Jashari, VM Adhami, JC Chamcheu, I Rady, M Sechi, H Mukhtar, IA Siddiqui Targeted nanoparticles encapsulating (-)-epigallocatechin-3-gallate for prostate cancer prevention and therapy Sci Rep, 2017;7(0):41573. 2017-01-01 [PMID: 28145499] (Mouse) Mouse
Howard H Bailey Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy Am J Clin Exp Urol, 2016;4(2):17-27. 2016-01-01 [PMID: 27766277] (Human) Human
Eswaraka J, Giddabasappa A, Han G, Lalwani K, Eisele K, Feng Z, Affolter T, Christensen J, Li G Axitinib and crizotinib combination therapy inhibits bone loss in a mouse model of castration resistant prostate cancer. BMC Cancer, 2014;14(0):742. 2014-01-01 [PMID: 25277255] (Mouse) Mouse
Windus L, Kiss D, Glover T, Avery V In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer. Exp Cell Res, 2012;318(19):2507-19. 2012-01-01 [PMID: 22841689] (Human) Human
Squillace RM, Miller D, Wardwell SD, Wang F, Clackson T, Rivera VM Synergistic activity of the mTOR inhibitor ridaforolimus and the antiandrogen bicalutamide in prostate cancer models. Int. J. Oncol., 2012;41(2):425-32. 2012-01-01 [PMID: 22614157] (Human) Human
Show All 16 Publications.

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Gene Symbol KLK3