Human Kallikrein 3/PSA Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human Kallikrein 3/PSA Quantikine ELISA Kit Summary

Background
The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quant...ikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.
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Specificity
Natural and recombinant human KLK3/PSA
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
KLK3

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DKK300.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Kallikrein 3/PSA Quantikine ELISA Kit

  • APS seminin
  • EC 3.4.21
  • EC 3.4.21.77
  • Gamma-seminoprotein
  • hK3
  • Kallikrein 3
  • kallikrein 3, (prostate specific antigen)
  • Kallikrein-3
  • kallikrein-related peptidase 3
  • KLK2A1
  • KLK3
  • P-30 antigen
  • prostate specific antigen
  • prostate-specific antigen
  • PSA semenogelase
  • PSA
  • Semenogelase
  • Seminin

Background

The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.

Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Kallikrein 3/PSA (DKK300)(13)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(9)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using DKK300 Applications Species
AL D'Souza, JR Chevillet, P Ghanouni, X Yan, M Tewari, SS Gambhir Tumor characterization by ultrasound-release of multiple protein and microRNA biomarkers, preclinical and clinical evidence PLoS ONE, 2018;13(3):e0194268. 2018 [PMID: 29547636] (Human) Human
MI Suominen, KM Fagerlund, JP Rissanen, YM Konkol, JP Morko, Z Peng, EJ Alhoniemi, SK Laine, E Corey, D Mumberg, K Ziegelbaue, SM Käkönen, J Halleen, RL Vessella, A Scholz Radium-223 inhibits osseous prostate cancer growth by dual targeting of cancer cells and bone microenvironment in mouse models Clin. Cancer Res., 2017;0(0):. 2017 [PMID: 28364014] (Mouse) Mouse
V Sanna, CK Singh, R Jashari, VM Adhami, JC Chamcheu, I Rady, M Sechi, H Mukhtar, IA Siddiqui Targeted nanoparticles encapsulating (-)-epigallocatechin-3-gallate for prostate cancer prevention and therapy Sci Rep, 2017;7(0):41573. 2017 [PMID: 28145499] (Mouse) Mouse
Howard H Bailey Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy Am J Clin Exp Urol, 2016;4(2):17-27. 2016 [PMID: 27766277] (Human) Human
Eswaraka J, Giddabasappa A, Han G, Lalwani K, Eisele K, Feng Z, Affolter T, Christensen J, Li G Axitinib and crizotinib combination therapy inhibits bone loss in a mouse model of castration resistant prostate cancer. BMC Cancer, 2014;14(0):742. 2014 [PMID: 25277255] (Mouse) Mouse
Windus L, Kiss D, Glover T, Avery V In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer. Exp Cell Res, 2012;318(19):2507-19. 2012 [PMID: 22841689] (Human) Human
Squillace RM, Miller D, Wardwell SD, Wang F, Clackson T, Rivera VM Synergistic activity of the mTOR inhibitor ridaforolimus and the antiandrogen bicalutamide in prostate cancer models. Int. J. Oncol., 2012;41(2):425-32. 2012 [PMID: 22614157] (Human) Human
Colquhoun, A J, Venier, N A, Vandersluis, A D, Besla, R, Sugar, L M, Kiss, A, Fleshner, N E, Pollak, M, Klotz, L H, Venkateswaran, V Metformin enhances the antiproliferative and apoptotic effect of bicalutamide in prostate cancer. Prostate Cancer Prostatic Dis, 2012;15(4):346-52. 2012 [PMID: 22614062] (Mouse) Mouse
Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M Sensitive plasma protein analysis by microparticle-based proximity ligation assays. Mol. Cell Proteomics, 2010;9(2):327-35. 2010 [PMID: 19955079] (Human) Human
Stan SD, Singh SV Transcriptional repression and inhibition of nuclear translocation of androgen receptor by diallyl trisulfide in human prostate cancer cells. Clin. Cancer Res., 2009;15(15):4895-903. 2009 [PMID: 19622577] (Human) Human
Show All 13 Publications.

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FAQs for Kallikrein 3/PSA (DKK300). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DKK300

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Bioinformatics

Gene Symbol KLK3