Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2)

Images

 
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
156C135.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.
Modification
p Ser213, p Ser210
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-4 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemistry-Paraffin reported in literature (Lin et al, 2007).
Publications
Read Publications using
NB100-56603 in the following applications:

  • 2 publications
  • WB
    5 publications

Reactivity Notes

New World Monkey. Predicted cross-reactivity with Chimpanzee, Dog, Mouse, Pig and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2)

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

The androgen receptor (AR) is an approx. 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790 (Lin et al. 2003). The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5415
Species: Hu
Applications: WB, IHC, ICC
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF4036
Species: Hu
Applications: WB, Simple Western
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-07799
Species: Hu
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-1596
Species: Hu, Pm, Rb
Applications: WB, ICC/IF, IHC
NB200-305
Species: Hu, Mu, Mk
Applications: WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02164
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00000126-D01P
Species: Hu, Mu
Applications: WB
NBP1-45335
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-90233
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
Supplier Logo

Publications for Androgen R/NR3C4 Antibody (NB100-56603)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(2)
WB
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-56603 Applications Species
Zellweger T, Sturm S, Rey S et al. Estrogen receptor B expression and androgen receptor phosphorylation correlate with a poor clinical outcome in hormone-naive prostate cancer and are elevated in castration-resistant disease. Endocr Relat Cancer. 2013 May 21 [PMID: 23580588]
Chen S, Gulla S, Cai C, Balk SP. Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation. J Biol Chem. 2012 Mar 9 [PMID: 22275373] (WB, Human)

Details:
Product cited: Androgen Receptor Ser213/210 (IMG-561). 1. WB: Human prostate cancer cells (LNCap) treated with nocodazole (NOCO) and CDK1 inhibitors (CGP74514A or RO-3306), Fig 1B.
WB Human
Kasina S, Macoska JA. The CXCL12/CXCR4 axis promotes ligand-independent activation of the androgen receptor. Mol Cell Endocrinol. 2012 Apr 4 [PMID: 22245379]
Varisli L, Gonen-Korkmaz C, Syed HM et al. Androgen regulated HN1 leads proteosomal degradation of androgen receptor (AR) and negatively influences AR mediated transactivation in prostate cells. Mol Cell Endocrinol. 2012 Mar 5 [PMID: 22155408] (WB) WB
Cai H, Smith DA, Memarzadeh S et al. Differential transformation capacity of Src family kinases during the initiation of prostate cancer. Proc Natl Acad Sci U S A. 2011 Apr 19 [PMID: 21464326] (IHC-P, Mouse)

Details:
Product cited: Androgen Receptor Ser213/210 (IMG-561). IHC (paraffin): Mouse urogenital sinus mesenchyme (UGSM) tissue, Fig 2.
IHC-P Mouse
Paliouras M, Diamandis EP. An AKT activity threshold regulates androgen-dependent and androgen-independent PSA expression in prostate cancer cell lines. Biol Chem. 2008 Jun [PMID: 18627304] (WB, Human)

Details:
Product cited: Androgen Receptor Ser213/210 (IMG-561). WB: Human prostate [PC3(AR)] cancer cell line, Fig 6.
WB Human
Palazzolo I, Burnett BG, Young JE et al. Akt blocks ligand binding and protects against expanded polyglutamine androgen receptor toxicity. Hum Mol Genet. 2007 Jul 1 [PMID: 17470458] (WB)

Details:
WB: Cos cells transfected or mock-transfected with AR sequences: Fig B-D.
WB
Bhuiyan MM, Li Y, Banerjee S et al. Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells. Cancer Res. 2006 Oct 15 [PMID: 17047070]
Lin SL, Chang D, Ying SY. Hyaluronan stimulates transformation of androgen-independent prostate cancer. Carcinogenesis. 2007 Feb [PMID: 16864594] (IHC-P)

Details:
Products cited: 1. Prostate Cancer Tissue Microarray (IMH-303): IHC (paraffin), Figs 2D, 4B and 6. The Androgen Receptor (Ser213/210) mAb (IMG-561) is used in Fig 6 2. Androgen Receptor (Ser213/210) mAb (IMG-561): IHC (paraffin), Fig 6. This antibody is u
IHC-P
Kirkegaard T, Edwards J, Tovey S et al. Observer variation in immunohistochemical analysis of protein expression, time for a change? Histopathology. 2006 Jun [PMID: 16722926]
Show All 12 Publications.

Reviews for Androgen R/NR3C4 Antibody (NB100-56603) (0)

There are no reviews for Androgen R/NR3C4 Antibody (NB100-56603). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Androgen R/NR3C4 Antibody (NB100-56603) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56603AF405
Alexa Fluor 488 Labeled NB100-56603AF488
Alexa Fluor 647 Labeled NB100-56603AF647
Alexa Fluor 700 Labeled NB100-56603AF700
Biotin Labeled NB100-56603B
DyLight 350 Labeled NB100-56603UV
DyLight 405 Labeled NB100-56603V
DyLight 488 Labeled NB100-56603G
DyLight 550 Labeled NB100-56603R
DyLight 650 Labeled NB100-56603C
DyLight 680 Labeled NB100-56603FR
DyLight 755 Labeled NB100-56603IR
HRP Labeled NB100-56603H

Additional Androgen R/NR3C4 Products

Bioinformatics Tool for Androgen R/NR3C4 Antibody (NB100-56603)

Discover related pathways, diseases and genes to Androgen R/NR3C4 Antibody (NB100-56603). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Androgen R/NR3C4 Antibody (NB100-56603)

Discover more about diseases related to Androgen R/NR3C4 Antibody (NB100-56603).
 

Pathways for Androgen R/NR3C4 Antibody (NB100-56603)

View related products by pathway.

PTMs for Androgen R/NR3C4 Antibody (NB100-56603)

Learn more about PTMs related to Androgen R/NR3C4 Antibody (NB100-56603).
 

Research Areas for Androgen R/NR3C4 Antibody (NB100-56603)

Find related products by research area.

Blogs on Androgen R/NR3C4

There are no specific blogs for Androgen R/NR3C4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) and receive a gift card or discount.

Bioinformatics

Gene Symbol AR
Entrez
Uniprot