GR/NR3C1 Antibody (BuGR2)

Images

 
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid ...read more
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in HeLa Cells. Glucocorticoid ...read more
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GP, Rb, Sh, YeSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
Clone
BuGR2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

GR/NR3C1 Antibody (BuGR2) Summary

Immunogen
Partially purified rat GR.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR3C1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:10 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Block/Neutralize
Application Notes
Blocking, ChIP, and ELISA usages were reported in scientific literature. Use in ICC/IF was reported in scientific literature (PMID: 30402116)
Control
GR/NR3C1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 3
using
NB300-731 in the following applications:

Publications
Read Publications using
NB300-731 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for GR/NR3C1 Antibody (BuGR2)

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GR/NR3C1 Antibody (NB300-731)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, Flow, ICC/IF, WB.


Filter By Application
ChIP
(1)
Flow
(1)
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB300-731 Applications Species
Adams R, Smith C Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny Mediators of Inflammation Aug 25 2019 [PMID: 31582905] (Flow, Mouse) Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny. Mediators Inflamm. 2019 Aug 25 [PMID: 31582905] (Flow, Mouse)

Details:
Citation using the Alexa Fluor 647 version of this antibody.
Flow Mouse
Schoenfelder Y, Hiemke C, Schmitt U. Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms. J Neuroendocrinol 2012 May [PMID: 22339976] (Mouse) Mouse
Harrell JM, Murphy PJ, Morishima Y et al. Evidence for glucocorticoid receptor transport on microtubules by dynein. J Biol Chem. 2004 Dec. [PMID: 15485845]
Viegas LR, Vicent GP, Baranao JL et al. Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4. J Biol Chem. 2004 Mar. [PMID: 14679196]
Lambert JR, Nordeen SK. CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins. Mol Endocrinol. 2003 Jun. [PMID: 12637584]
Taves MD, Mittelstadt PR, Presman DM et al. Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus Cell Rep Mar 26 2019 [PMID: 30917317] (Mouse) Mouse
Roh Kyung-Baeg, Park Deokhoon, Jung Eunsun et al Inhibitory Effects of Prunella vulgaris L. Extract on 11 beta-HSD1 in Human Skin Cells. Evid Based Complement Alternat Med. 2018 Oct 4 [PMID: 30402116] (ICC/IF, Human)

Details:
Citation using the PE version of this antibody.
ICC/IF Human
Rue L, Banez-Coronel M, Creus-Muncunill J et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J. Clin. Invest. Nov 1 2016 [PMID: 27721240] (WB, Mouse) WB Mouse
Wu JN, Pinello L, Yissachar E et al. Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia. Epigenetics Chromatin 2015 [PMID: 26633995] (ChIP) ChIP

Reviews for GR/NR3C1 Antibody (NB300-731) (3) 33

Average Rating: 3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Equine.

Reviews using NB300-731:
Filter by Applications
IHC-P
(1)
WB
(2)
All Applications
Filter by Species
Mouse
(1)
Equine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GR/NR3C1 NB300-731
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reviewed by:
Shavahn Loux
IHC-P Equine 02/01/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver tissue,Liver
SpeciesEquine
LotUH286741

Comments

CommentsLow Rating Note: Novus Innovators Program - new species or application used on a primary antibody.

Tried HIER ph 6 and HIER pH 9 with concentrations as high as 1:200. Never saw any nuclear staining in horse liver; only non-specific background staining.
Western Blot GR/NR3C1 NB300-731
Enlarge
reviewed by:
Agnes Villers
WB Mouse 09/03/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse hippocampal tissue lysate
SpeciesMouse
Lot0K194127
CommentsProteins were extracted from hippocampal tissue in extraction buffer (Tris-HCl 50mM (pH 6.8), SDS 0.1%, Triton X-100 0.1 %, EDTA 2mM, protease inhibitors) and protein amount was assessed with BCA method. Electrophoresis was carried out using 10ug of protein extract with a 4-15% Mini-Protean TGX gel and TGX (Tris glycine SDS 10X) running buffer. Duplicate samples of hippocampal extract were loaded two lanes. Proteins were transfered on nitrocellulose membrane (wet conditions, Tris-glycine buffer with 20% methanol). 0.2ug/mL glucocorticoid receptor antibody (NB300-731) was used in 5% BSA TBST and incubated overnight at 4-degrees. The secondary antibody (GE healthcare) was used at 1:10000. Loading control was evaluated using anti-GAPDH antibody.

Blocking

Blocking DetailsBlocking buffer: 5 % BSA in TBST, blocking Time: 1 hour, Blocking temperature: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 0,2 μg/ml, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: ECL Anti-mouse Ig, Horseradish (from sheep), Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#GE Healthcare NA931V
Secondary Concentration1:10000

Details

Detection NotesDetection Method: ECL, Exposure Time: around 30s, Pos Ctrl: none, Neg Ctrl: None, Specific Band: around 90 kDa

Comments

CommentsProteins were extracted from hippocampal tissue in extraction buffer (Tris-HCl 50mM (pH 6.8), SDS 0.1%, Triton X-100 0.1 %, EDTA 2mM, protease inhibitors) and protein amount was assessed with BCA method. Electrophoresis was carried out using 10ug of protein extract with a 4-15% Mini-Protean TGX gel and TGX (Tris glycine SDS 10X) running buffer. Duplicate samples of hippocampal extract were loaded two lanes. Proteins were transfered on nitrocellulose membrane (wet conditions, Tris-glycine buffer with 20% methanol). 0.2ug/mL glucocorticoid receptor antibody (NB300-731) was used in 5% BSA TBST and incubated overnight at 4-degrees. The secondary antibody (GE healthcare) was used at 1:10000. Loading control was evaluated using anti-GAPDH antibody.
Western Blot GR/NR3C1 NB300-731
Enlarge
reviewed by:
Agnes Villers
WB 08/29/2014
View

Summary

ApplicationWestern Blot
Lot0K194127

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GR/NR3C1 Antibody (NB300-731) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-731AF350
Alexa Fluor 405 NB300-731AF405
Alexa Fluor 488 NB300-731AF488
Alexa Fluor 532 NB300-731AF532
Alexa Fluor 594 NB300-731AF594
Alexa Fluor 647 NB300-731AF647
Alexa Fluor 700 NB300-731AF700
Alexa Fluor 750 NB300-731AF750
Allophycocyanin NB300-731APC
DyLight 350 NB300-731UV
DyLight 405 NB300-731V
DyLight 488 NB300-731G
DyLight 550 NB300-731R
DyLight 594 NB300-731DL594
DyLight 650 NB300-731C
DyLight 680 NB300-731FR
DyLight 755 NB300-731IR
Janelia Fluor 549 NB300-731JF549
Janelia Fluor 646 NB300-731JF646
PE NB300-731PE
PerCP NB300-731PCP

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Recent Reviews

3
5
0
4
2
3
0
2
0
1
1

Shavahn Loux
02/01/2020
Application: IHC-P
Species: Equine

Agnes Villers
09/03/2014
Application: WB
Species: Mouse

Agnes Villers
08/29/2014
Application: WB
Species:

Bioinformatics

Gene Symbol NR3C1
Entrez
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