GR/NR3C1 Antibody (BuGR2)

Images

 
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid ...read more
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in Hela Cells. Glucocorticoid ...read more
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in Hela cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, GP, Rb, Sh, YeSpecies Glossary
Applications WB, B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
Clone
BuGR2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

GR/NR3C1 Antibody (BuGR2) Summary

Immunogen
Partially purified rat GR.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
Isotype
IgG2
Clonality
Monoclonal
Host
Mouse
Gene
NR3C1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:1- 1:100
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Blocking, ChIP, and ELISA usages were reported in scientific literature.
Control Peptide
GR/NR3C1 Protein (NB300-610PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB300-731 in the following applications:

Publications
Read Publications using
NB300-731 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for GR/NR3C1 Antibody (BuGR2)

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP

Publications for GR/NR3C1 Antibody (NB300-731)(7)

Reviews for GR/NR3C1 Antibody (NB300-731) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 review tested in 1 species: Mouse.

Reviews using NB300-731:
Filter by Applications
WB
(2)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot GR/NR3C1 NB300-731
Enlarge
reviewed by:
Agnes Villers
WB Mouse 09/03/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse hippocampal tissue lysate
SpeciesMouse
Lot0K194127
CommentsProteins were extracted from hippocampal tissue in extraction buffer (Tris-HCl 50mM (pH 6.8), SDS 0.1%, Triton X-100 0.1 %, EDTA 2mM, protease inhibitors) and protein amount was assessed with BCA method. Electrophoresis was carried out using 10ug of protein extract with a 4-15% Mini-Protean TGX gel and TGX (Tris glycine SDS 10X) running buffer. Duplicate samples of hippocampal extract were loaded two lanes. Proteins were transfered on nitrocellulose membrane (wet conditions, Tris-glycine buffer with 20% methanol). 0.2ug/mL glucocorticoid receptor antibody (NB300-731) was used in 5% BSA TBST and incubated overnight at 4-degrees. The secondary antibody (GE healthcare) was used at 1:10000. Loading control was evaluated using anti-GAPDH antibody.

Blocking

Blocking DetailsBlocking buffer: 5 % BSA in TBST, blocking Time: 1 hour, Blocking temperature: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 0,2 μg/ml, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: ECL Anti-mouse Ig, Horseradish (from sheep), Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#GE Healthcare NA931V
Secondary Concentration1:10000

Details

Detection NotesDetection Method: ECL, Exposure Time: around 30s, Pos Ctrl: none, Neg Ctrl: None, Specific Band: around 90 kDa

Comments

CommentsProteins were extracted from hippocampal tissue in extraction buffer (Tris-HCl 50mM (pH 6.8), SDS 0.1%, Triton X-100 0.1 %, EDTA 2mM, protease inhibitors) and protein amount was assessed with BCA method. Electrophoresis was carried out using 10ug of protein extract with a 4-15% Mini-Protean TGX gel and TGX (Tris glycine SDS 10X) running buffer. Duplicate samples of hippocampal extract were loaded two lanes. Proteins were transfered on nitrocellulose membrane (wet conditions, Tris-glycine buffer with 20% methanol). 0.2ug/mL glucocorticoid receptor antibody (NB300-731) was used in 5% BSA TBST and incubated overnight at 4-degrees. The secondary antibody (GE healthcare) was used at 1:10000. Loading control was evaluated using anti-GAPDH antibody.
Western Blot GR/NR3C1 NB300-731
Enlarge
reviewed by:
Agnes Villers
WB 08/29/2014
View

Summary

ApplicationWestern Blot
Lot0K194127

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GR/NR3C1 Antibody (NB300-731) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-731AF405
Alexa Fluor 488 Labeled NB300-731AF488
Alexa Fluor 647 Labeled NB300-731AF647
Alexa Fluor 700 Labeled NB300-731AF700
Allophycocyanin Labeled NB300-731APC
DyLight 350 Labeled NB300-731UV
DyLight 405 Labeled NB300-731V
DyLight 488 Labeled NB300-731G
DyLight 680 Labeled NB300-731FR
DyLight 755 Labeled NB300-731IR
PE Labeled NB300-731PE
PerCP Labeled NB300-731PCP

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Agnes Villers
09/03/2014
Application: WB
Species: Mouse

Agnes Villers
08/29/2014
Application: WB
Species:

Bioinformatics

Gene Symbol NR3C1
Entrez