GR/NR3C1 Antibody (BuGR2) - BSA Free

Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in HeLa Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

• Reactivity: Hu, Mu, Rt, Gp, Rb, Sh, Ye
• Applications: WB, Flow, GS, ICC/IF, IHC, IP, B/N, ChIP
• Clone: BuGR2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

GR/NR3C1 Antibody (BuGR2) - BSA Free Summary

• Immunogen: Partially purified rat GR.
• Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
• Specificity: Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR3C1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:10 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 5 ug/mL
• Application Notes: Blocking, ChIP, and ELISA usages were reported in scientific literature. Use in ICC/IF was reported in scientific literature (PMID: 30402116)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for GR/NR3C1 Antibody (BuGR2) - BSA Free

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GR/NR3C1 Antibody (NB300-731)(12)

Publications using NB300-731

• Rudak PT, Choi J, Parkins KM et al. Chronic stress physically spares but functionally impairs innate-like invariant T cells Cell Rep 2021-04-14 [PMID: 33852855]
  
  Details:
  Citation using the PE format of this antibody.
• Adams RCM, Smith C. In utero Exposure to Maternal Chronic Inflammation Transfers a Pro-Inflammatory Profile to Generation F2 via Sex-Specific Mechanisms Front Immunol. [PMID: 32117231] (ICC/IF, Mouse)
• Adams R, Smith C Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny Mediators of Inflammation Aug 25 2019 [PMID: 31582905] (Flow, Mouse) Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny. Mediators Inflamm. 2019-08-25 [PMID: 31582905] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Taves MD, Mittelstadt PR, Presman DM et al. Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus Cell Rep 2019-03-26 [PMID: 30917317] (Mouse)
• Roh Kyung-Baeg, Park Deokhoon, Jung Eunsun et al Inhibitory Effects of Prunella vulgaris L. Extract on 11 beta-HSD1 in Human Skin Cells. Evid Based Complement Alternat Med. 2018-10-04 [PMID: 30402116] (ICC/IF, Human)
  
  Details:
  Citation using the PE version of this antibody.
• Rue L, Banez-Coronel M, Creus-Muncunill J et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J. Clin. Invest. 2016-11-01 [PMID: 27721240] (WB, Mouse)
• Wu JN, Pinello L, Yissachar E et al. Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia. Epigenetics Chromatin 2015-01-01 [PMID: 26633995] (Chemotaxis)
• Schoenfelder Y, Hiemke C, Schmitt U. Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms. J Neuroendocrinol 2012-05-01 [PMID: 22339976] (Mouse)
• Harrell JM, Murphy PJ, Morishima Y et al. Evidence for glucocorticoid receptor transport on microtubules by dynein. J Biol Chem. 2004-12-01 [PMID: 15485845]
• Viegas LR, Vicent GP, Baranao JL et al. Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4. J Biol Chem. 2004-03-01 [PMID: 14679196]
• Lambert JR, Nordeen SK. CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins. Mol Endocrinol. 2003-06-01 [PMID: 12637584]

Reviews for GR/NR3C1 Antibody (NB300-731) (3)

Average Rating: 3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Equine.

reviewed by: Verified Customer IHC-P Equine 02/01/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver tissue,Liver
• Species: Equine
• Lot: UH286741

Comments

• Comments: Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
  
  Tried HIER ph 6 and HIER pH 9 with concentrations as high as 1:200. Never saw any nuclear staining in horse liver; only non-specific background staining.

reviewed by: Verified Customer WB Mouse 09/03/2014

Summary

• Application: Western Blot
• Sample Tested: mouse hippocampal tissue lysate
• Species: Mouse
• Lot: 0K194127

reviewed by: Verified Customer WB 08/29/2014

Summary

• Application: Western Blot
• Lot: 0K194127

Bioinformatics

• Gene Symbol: NR3C1
• Entrez:
  • 2908(Human)
  • 14815(Mouse)
  • 24413(Rat)