AKR1A1 Antibody (OTI6E3)

Images

 
Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.
Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal, and then ...read more
Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] - Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody.
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI6E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.74 mg/ml

Order Details

AKR1A1 Antibody (OTI6E3) Summary

Immunogen
Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AKR1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.74 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKR1A1 Antibody (OTI6E3)

  • AKR1A1
  • alcohol dehydrogenase [NADP+]
  • alcohol dehydrogenase
  • Aldehyde reductase
  • Aldo-keto reductase family 1 member A1
  • aldo-keto reductase family 1, member A1 (aldehyde reductase)
  • ALDR1
  • ALRMGC1380
  • ARM
  • DD3
  • dihydrodiol dehydrogenase 3
  • EC 1.1.1
  • EC 1.1.1.19
  • EC 1.1.1.2
  • EC 1.1.1.20
  • EC 1.1.1.372
  • EC 1.1.1.54
  • MGC12529

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AKR1A1 Antibody (NBP2-02164) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKR1A1
Entrez