AIF-1/Iba1 Antibody


Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various whole 30 ug cell extracts (Molecular weight: 16.7 KDa) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at a dilution more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse thymus gland stained by AIF-1/Iba1 Antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of AIF-1/Iba1 protein from K562 whole cell extracts using 5ug of AIF-1/Iba1 Antibody Western blot analysis was performed using Iba1 antibody more
Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: AIF1 protein stained by AIF-1/Iba1 Antibody diluted at 1:500. Blue: Hoechst 33343 more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight more
Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted by 1:500.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-16908] - Frozen sectioned E13.5 Rat brain. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded rat brain stained using AIF-1/Iba1 Antibody diluted at 1:500.
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).

Product Details

Reactivity Hu, Mu, Rt, Po, RMSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

AIF-1/Iba1 Antibody Summary

This AIF-1/Iba1 Antibody was developed against a recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.
pan-Microglia Marker
Predicted Species
Porcine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-16908 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Bovine (88%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1


Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.


1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA.

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters.

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation.

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells.

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NBP2-16908)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol AIF1