AIF-1/Iba1 Antibody

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Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: AIF1 protein stained by Iba1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded rat brain. Iba1 antibody diluted at 1:500.
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with Iba1 antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).
Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various whole cell extracts (30ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at a dilution of 1:5000.
Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted by 1:500.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded breast cancer stroma. Iba1 antibody dilution: 1:500.
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-16908] - Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Paraffin-embedded mouse brain.Iba1 antibody diluted at 1:500.
Immunoprecipitation: AIF-1/Iba1 Antibody [NBP2-16908] - Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5ug of Iba1 antibody Western blot analysis was performed using Iba1 antibody EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.56 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.
Marker
pan-Microglia Marker
Predicted Species
Porcine (93%), Monkey (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-16908 in the following applications:

Reactivity Notes

Rhesus Monkey (99%), Bovine (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.56 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • AIF-1IRT1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IBA1Em:AF129756.17
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NBP2-16908)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot