Flow cytometry

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Flow Cytometry Basics for the Non-Expert

Application Focus: I see an increase in LC3, now what?

Applications Guide: How to choose fluorophore combinations for Flow Cytometry

Flow cytometry was developed to label and examine single cells with high throughput capacity using antibodies conjugated to fluorophores. The basic concept of flow cytometry is that a cell suspension is delivered as a single stream and is passed through a light source that uses detectors to generate data sets based off cellular properties. More specifically, the light emitted by fluorescently conjugated antibodies is channeled through selected filters to sort based off preset parameters or targets used.