Immunohistochemistry

Dual ISH-IHC: Better Together

Neurovascular signaling for repair enhances brain metastasis

Explore IHC basics with this new guide: Immunohistochemistry Essential Elements and Beyond

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation

The importance of using controls in immunohistochemistry

Immunohistochemistry (IHC) is a widely applied experimental method used to examine tissue antigen expression and behavior with the use of an antibody conjugated to a secondary tag for visualization.  IHC consists of a tissue preparation phase, an antibody-staining phase, and a result analysis phase - all of which may lead to skewed results if not properly performed.  One way to ensure that IHC staining results are in fact demonstrating protein behavior and not a side effect the experimental process is to use IHC controls within each expe

Include tissue controls in every IHC experiment to instill confidence in your results

Interpretation of immunohistochemistry (IHC) data is difficult in the absence of appropriate controls.

To confirm staining specificity and instill confidence in your results, a positive and negative tissue control should be routinely included in IHC experiments.

Webinar: Multi-color fluorescence immunohistochemistry using primary antibodies raised in the same host species

Fluorescent probes conjugated to antibodies allow for simultaneous IHC detection of multiple antigens in the same tissue section. However, quite often conventional multi-color IHC cannot be done if only primary antibodies raised in the same host species are available to the researcher. To solve this problem, we developed a novel technique for performing multicolor fluorescence immunohistochemistry using primary antibodies derived from a single host source.

LC3B: From Autophagy to Cancer

LC3B is subunit component of the LC3 autophagy biomarker associated with microtubule-associated proteins MAP1A and MAP1B and one of the best characterized markers to date. In resting state, it is cytosolic, but upon activation, is lapidated and becomes embedded in the autophagosomal membrane.

Fluorescent Dyes And Conjugated Antibodies

Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.

Explaining Conjugated Antibodies

A conjugated antibody (also known as a tagged, loaded or labeled antibody) is one which has been attached to a substrate such as an enzyme, toxin or inorganic compound. Modern immunoassay techniques make extensive use of proteins conjugated with fluorescent dye.