Immunofluorescence

Make each cell count: How to assess autophagy using flow cytometry

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease

Why do counterstaining in ICC/IF and how?

Why: To identify a specific organelle or another cellular structure and to mark individual cells, it is necessary to counterstain them in immunocytochemistry/immunofluorescence (ICC/IF) assays.

Five key tips for a better multicolor immunofluorescence staining

  1. Multicolor immunofluorescence staining is best carried out by sequentially incubating cells with unlabeled-primary and labeled-secondary antibodies. However when options are limited, it may also be performed by simultaneous incubation of cells with directly labelled primary antibodies.  

Breakdown: Interpreting LC3 Antibody WB Results

In rodents, MAP1LC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form, LC3II, following conversion from LC3I.

Time to Shine! - Developments in 30 Years of Western Blotting Technology

The vast majority of antibodies in our antibody catalog are suitable for Western blotting studies. Devised almost 30 years ago by W. Neal Burnette, it has become a standard assay wherever antibodies are used to detect proteins.

Bright & Colorful: Fluorescent Markers and FACS Assays

As a top worldwide antibody supplier, we at Novus Biologicals are constantly extending our antibody database to take advantage of the latest technology and product developments. Recently, we added several embryonic stem cell marker antibodies, conjugated for use in FACS (fluorescence activated cell sorting) assays.

Fluorescence Activated Cell Sorting Antibody Techniques

Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and GPR49/LGR5 antibodies.

Fluorescent Dyes And Conjugated Antibodies

Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.

Explaining Conjugated Antibodies

A conjugated antibody (also known as a tagged, loaded or labeled antibody) is one which has been attached to a substrate such as an enzyme, toxin or inorganic compound. Modern immunoassay techniques make extensive use of proteins conjugated with fluorescent dye.