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Chloroquine

Autophagic flux: Is p62 a good indicator?

p62 detection via IHC-Paraffin, Novus Biologicals

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3-II
LC3B
p62/SQSTM1
alpha Tubulin
mCherry
Autophagic flux
Chloroquine
Bafilomycin-A1
ATG5
ATG7
PKC zeta
NFkB
Keap1
NRF2
ERK1
Christina Towers

Best genes to knockout to control autophagic flux

Autophagy interactive signaling pathway, Novus Biologicals, macroautophagy induction, autophagosome maturation, microautophagy, autolysosome, elongation

By Christina Towers, PhD

Tags: 
Autophagy
Autophagic flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy Inhibitors
ATGs
ATG5
ATG7
Atg3
ATG12
stx17
p62-sqstm1
ATG13
Apoptosis
Christina Towers
autophagosome
Autophagy flux

Make each cell count: How to assess autophagy using flow cytometry

LC3B expression in HeLa cells treated with chloroquine overnight, ICC. Novus Biologicals

Kristy R. Howell, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
alpha Tubulin
GFP
mCherry
Autophagy flux
Chloroquine
rapamycin
Flow cytometry
Kristy Howell
Jurkat cell
MIFC
puncta
fluorescence
Immunocytochemistry
Immunofluorescence

How to visualize autophagy by microscopy

LC3B and alpha tubulin expression in HeLa cells treated with Chloroquine, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
p62/SQSTM1
alpha Tubulin
GFP
Mitophagy
mCherry
Autophagic flux
Chloroquine
Christina Towers
Autophagy flux
microscopy

The LC3 A, B, C’s and 1, 2, 3’s

Atg5 expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Autophagy
Autophagy Inhibitors
ATG4
ATG5
ATG12
LC3A
LC3C
GABARAP
GABARAPL1
GABARAPL2
Chloroquine
Bafilomycin-A1
ATG7
ATG8
Christina Towers
autophagosome

Best way to quantitatively measure Autophagic Flux

LC3B expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy
Autophagy Inhibitors
Christina Towers
autophagosome
GFP
Flow cytometry
ratiometric
RFP
mCherry

Best Methods to Induce and Inhibit Autophagy Pharmacologically

VPS35 expression in endosomes and lysosomes of human U-2 OS cells, ICC

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
Bafilomycin
Chloroquine
Lysosome
Western blot
Beclin 1
VPS34
ULK1
ATG4B
mTOR
autophagy induction
autophagy inhibition
Christina Towers
Cancer
rapamycin
Autophagic flux

Application Focus: I see an increase in LC3, now what?

LC3A expression in HeLa cells, chloroquine treated, ICC

 

By Christina Towers, PhD.

 

Tags: 
Autophagy
autophagosome
Transcription Regulation
LC3
LC3B
Bafilomycin
Chloroquine
beta-Actin
Lysosome
Western blot
mCherry
Autophagy flux
Flow cytometry
LC3-II
Christina Towers

Measuring Autophagic Flux with LC3 protein levels: The do's and don'ts

Expression of p62/SQSTM1 in HeLa cells mock- or chloroquine-treated, ICC

By Christina Towers, PhD.

Tags: 
Autophagy
autophagosome
Lysosome
LC3
p62/SQSTM1
Chloroquine
Autophagic flux

Autophagy Inhibition in Cancer: Clinical Trials Update

Autophagy signaling pathway

By Christina Towers, PhD.

Tags: 
Autophagy
autophagosome
Lysosome
Cancer
Tumor
Chloroquine
Hydroxychloroquine
Beclin 1

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