Make each cell count: How to assess autophagy using flow cytometry

Rules for Selection of Fluorochromes in Multicolor ICC/IF

Immunocytochemistry/immunofluorescence (ICC/IF) involves visualization of antigens in cultured or smeared cells with the use of fluorochrome-labeled antibodies. Various combinations of fluorochrome-labeled antibodies make it possible to simultaneously detect multiple antigens in the same sample. However, every experiment involving multiplex or multicolor ICC/IF requires selection of an optimal set of fluorochromes.

Why do counterstaining in ICC/IF and how?

Why: To identify a specific organelle or another cellular structure and to mark individual cells, it is necessary to counterstain them in immunocytochemistry/immunofluorescence (ICC/IF) assays.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers

The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants LC3A, LC3B, and LC3C.1 LC3B is a subunit of the MAP1A and MAP1B microtubule-binding proteins and plays a central role in autophagosome membrane structure.

Newly Validated Antibodies for Immunocytochemistry

Immunocytochemistry (ICC) is a frequently used technique that allows researchers to determine if an antigen is expressed by specific cellular or sub-cellular compartments via the use of antibodies. Many research-grade antibodies are suitable for ICC analysis, however many are not or have not been tested.