| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1475SS | |||
| Azide and BSA Free | NBP2-80640 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-64775-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44513 | |||
| Azide and BSA Free | NBP2-34569-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1642SS | |||
| Azide and BSA Free | NBP2-80639 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65805SS | |||
| Azide and BSA Free | NBP2-80660 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47386SS | |||
| Azide and BSA Free | NBP2-80650 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-534SS | |||
| Azide and BSA Free | NBP2-80947 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71663SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64895SS | |||
| Azide and BSA Free | NBP2-80651 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-504-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27216 | |||
| Azide and BSA Free | NBP2-25199SS | |||
| Azide and BSA Free | NBP2-27216-25ug | |||
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] (NB600-1475) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0019.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] (NB600-1475) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - 4T1 tumor sections were stained for CD31 (green) and CD105 (red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Frozen-NB600-1475-img0006.jpg)
![Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cell-Surface-NB600-1475-img0009.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified CD31/PECAM-1 Antibody (MEC13.3), followed by anti-rat IgG FITC](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cytometry-NB600-1475-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to DyLight 488 (NB600-1475G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0011.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - JAK3 colocalized with endothelial cells labeled with CD31. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fneur.2017.00363/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Paraffin-NB600-1475-img0015.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cytometry-NB600-1475-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to Alexa Fluor 647 (NB600-1475AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to DyLight 550 (NB600-1475R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunocytochemistry-Immunofluorescence-NB600-1475-img0018.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Frozen-NB600-1475-img0012.jpg)
![Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cell-Surface-NB600-1475-img0013.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cytometry-NB600-1475-img0014.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475DL594 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 594.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cytometry-NB600-1475-img0020.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475G (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC13-3-Flow-Cytometry-NB600-1475-img0021.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1475_rat-monoclonal-cd31-pecam-1-antibody-mec13-3-310202416171428.jpg)
![Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Immunohistochemistry-Frozen-NB100-64775-img0015.jpg "Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.")
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0013.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0002.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0004.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0006.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0007.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0008.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0009.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0010.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were](http://images.novusbio.com/fullsize/HLA-ABC-Antibody-W6-32-Flow-Cytometry-NB100-64775-img0014.jpg)
![Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Knockout-Validated-NBP2-25208-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution and proportion of CD86+ macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (d). The results were expressed as Mean +/- SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD and OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS and ##; P < 0.01 vs either NS or OVA/BUD group. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-020-1322-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green), M1 marker CD86 (red) and DAPI (blue) (n = 5, respectively). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fphar.2018.00464/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-NBP2-25208-img0010.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0008.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BU63-Flow-Cytometry-NBP2-25208-img0007.jpg)
![Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution & proportion of CD68+ (a), CD86+ (c) & CD163+ (e) macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (b, d, f). The results were expressed as Mean ± SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD & OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS & ##; P < 0.01 vs either NS or OVA/BUD group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32111211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241534324.jpg)
![Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE–/– mice. (A,B) Co-immunofluorescence staining of the aortic root for macrophage (anti-Moma-2 antibody, green) & M1 marker iNos (red), & a bar graph summarizing the results (n = 5, respectively). (C,D) Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green) & M1 marker CD86 (red), & a bar graph summarizing the results (n = 5, respectively). Scale bar: 20 μm. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29867472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-imgenex-img-6882a-25ug-31020241535650.jpg)
![M1 macrophages were induced in the in vivo mouse intervertebral disc (mIVD) degeneration model (puncture model). (a) Immunohistological analysis of Iba-1, iNOS, and arginase (brown staining) expression in nucleus pulposus (NP), annulus fibrosis (AF), and cartilage endplate (CEP) [right] at high magnification and in whole mIVDs (left) at low magnification. Images using an IgG isotype control antibody are included to demonstrate the specificity of the IHC staining. (b) Protein was extracted from punctured mIVDs in the puncture model, and western blotting was performed using antibodies against CD86, CD163, and beta -actin. (c) Images of the panel in (b), captured using a ChemiDoc Touch system and quantified using ImageJ. Biological and technical replicates were performed three times. Values represent the mean +/- SD. *p < 0.05, **p < 0.01, relative to the corresponding control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/41053148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25208_mouse-monoclonal-b7-2-cd86-antibody-bu63-western-blot-13220261327102.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0014.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] (NB100-1642) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS-1 Cells with CD31/PECAM-1 Antibody (MEC 7.46) NB100-1642(blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cell-Surface-NB100-1642-img0005.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31) and beta-amyloid comparing 13- and 24-month old arcAbeta mice. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to Alexa Fluor 488 (NB100-1642AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC 7.46] conjugated to DyLight 550 (NB100-1642R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0016.jpg)
![Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunocytochemistry-Immunofluorescence-NB100-1642-img0012.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0015.jpg)
![Immunohistochemistry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - Immunohistochemistry for vessel staining (CD31/PECAM-1) and beta-amyloid comparing 13- and 24-month old non-transgenic littermates. Overviews (scale bars = 200 um) of the corpus callosum (CC), thalamus (TH), and cerebellum (CB). Insets show the regions with higher magnification (scale bar = 50 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnagi.2015.00241/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Immunohistochemistry-NB100-1642-img0010.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM-1 Antibody (MEC 7.46) was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-MEC-7-46-Flow-Cytometry-NB100-1642-img0006.jpg)
![Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Western-Blot-NB100-65805-img0015.jpg "Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Immunohistochemistry-Paraffin-NB100-65805-img0003.jpg)
![Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-BSA-Free-Flow-Cytometry-NB100-65805-img0016.jpg)
![Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Western-Blot-NB100-65805-img0007.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0009.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0001.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0011.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0012.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0013.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD81-Antibody-1D6-Flow-Cytometry-NB100-65805-img0014.jpg)
![Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] - Characterization & internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate & M2-Exos preparation. (D,F) Fluorescence images of 786-O & ACHN cells treated with or without PKH67-labeled M2-Exos (green). Scale bar: 50 μm. (E,G) Three-dimensional confocal reconstruction of 786-O & ACHN cells treated with PKH67-labeled M2-Exos (green). (H) Fluorescence staining analyzing the internalization of M2-Exos by 786-O cells over 12 h. Scale bar: 10 μm. CT: control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65805_mouse-monoclonal-cd81-antibody-1d6-310202415382443.jpg)
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).")
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0018.jpg)
![Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of pSTAT3, STAT3, and CD44 (NBP1-47386) in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Western-Blot-NBP1-47386-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD44 Antibody [8E2F3] conjugated to Alexa Fluor 647 (NBP1-47386AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0019.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Cytometry-NBP1-47386-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0010.jpg)
![Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-Flow-Intracellular-NBP1-47386-img0016.jpg)
![Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/CD44-Antibody-8E2F3-BSA-Free-Immunoprecipitation-NBP1-47386-img0020.jpg)
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Immunohistochemistry-Frozen-NB600-534-img0003.jpg "Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.")
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Flow-Cytometry-NB600-534-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Sialoadhesin-Antibody-HSn-7D2-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.")
![Simple Western: CD31/PECAM-1 Antibody [NBP1-71663] - Image shows a specific band for CD31/PECAM 1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Simple-Western-NBP1-71663-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CD31/PECAM-1 Antibody [NBP1-71663] - 5-HTR1A agonist enhances neovascularization during skin wound healing (excisional punch biopsy model). CD31/PECAM-1 endothelial cell immunohistochemical staining at day 5 (A,B) and day 7 (E,F), image scale bars represented at 50 um. Results were presented as mean +/- SEM (n = 5 images per wound). Two-way ANOVA was performed and significance levels were set at *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01406/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunohistochemistry-NBP1-71663-img0012.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Snail and EndMT-related protein expression in lungs in orthotopic murine 4T1 breast cancer model. ag Expression of Snail (see Methods). Levels of CD31/PECAM-1 determined by western blot analysis in pooled (n = 6) samples in control and 4T1 breast cancer-bearing mice from the 1st to 5th week after 4T1 cancer cell inoculation (see Methods). Results presented as fold change vs control sample corresponding to healthy mice. Total protein after transfer was used as loading control Image collected and cropped by Citeab from the following publication (Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res (2018) licensed under a CC-BY license](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0011.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Flow-Cytometry-NBP1-71663-img0005.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0008.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0005.jpg "Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 antibody (OX-1). Staining of rat spleen cells.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0004.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) - BSA Free [NB100-64895] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-BSA-Free-Flow-Cytometry-NB100-64895-img0006.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 antibody (OX-1) [NB100-64895].](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours and probed with a NFATc2 monoclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0010.jpg "Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours and probed with a NFATc2 monoclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.")
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human spleen tissue tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0012.jpg)
![Chromatin Immunoprecipitation: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - IP performed using Matrix ChIP assay with 1.0ul/100ul well of NFATc2 mab. Chromatin aliquots from ~1x10^5 cells used per ChIP pull-down. Quantitative PCR data done x4 using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of LAMC1. PCR calibration curves generated per primer pair from a series of sheared total genomic DNA. ChIP presented relative to the total amount of input chromatin. Results represent mean +/- SEM for 3 experiments. Representation of rat LAMC1 locus shown above data where boxes represent exons (black = translated regions, white = untranslated regions); zigzag line represents an intron, & straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Chromatin-Immunoprecipitation-NB300-504-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in Hela Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in MCF-7 Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 using NFATc2 Monoclonal Antibody (25A10.D6.D2) shows staining in U251 Cells. NFATc2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc2 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunocytochemistry-Immunofluorescence-NB300-504-img0008.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0004.jpg)
![Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/NFATC2-NFAT1-Antibody-25A10-D6-D2-Immunohistochemistry-Paraffin-NB300-504-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0007.jpg "Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.")
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0004.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0009.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0010.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.](http://images.novusbio.com/fullsize/nbp2-25199_mouse-monoclonal-cd4-antibody-rpa-t4-imgenex-img-5916a-25ug-17720241044654.jpg)