CD81 Antibody (1D6)

Images

 
Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) [NB100-65805] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin ...read more
Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells ...read more
Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 ...read more
Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Gt, Pm, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD81 Antibody (1D6) Summary

Immunogen
Aggregated OCI-Ly8 human B cell line
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD81
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • ELISA
  • Flow Cytometry 0.2 - 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
NB100-65805 is a potent TAPA1/CD81 reagent, it induces homotypic adhesion and has powerful anti-proliferative effects. For Flow Cytometry: Use 10 uL of the suggested working dilution to label 106 cells in 100 uL. This antibody is CyTOF ready.
Control
CD81 Overexpression Lysate
Publications
Read Publications using
NB100-65805 in the following applications:

Reactivity Notes

Chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD81 Antibody (1D6)

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

TAPA1, also known as CD81, is found in the plasma membrane in lymphocytes and is believed to play an important role in the regulation of lymphoma cell growth. This protein is primarily found on B and T cells (lymphocytes) in humans. TAPA1 appears to be involved in a variety of cellular processes including activation, proliferation and differentiation. It is believed to act as a viral receptor for HCV and may play a crucial role in cell signaling from its place embedded in the plasma membrane. Mutations affecting CD81 can cause immunodeficiency- a disease that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections due to a lack of an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD81 Antibody (NB100-65805)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-65805 Applications Species
Otake K, Kamiguchi H, Hirozane Y. Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid BMC Med Genomics Jan 10 2019 [PMID: 30630471] (WB, Human) WB Human
Kibria G, Ramos EK, Lee KE et al. A rapid, automated surface protein profiling of single circulating exosomes in human blood. Sci Rep. Nov 7 2016 [PMID: 27819324] (WB, Human) WB Human
Tian ZF, Shen H, Fu XH et al. Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81. World J Gastroenterol. 2009 Jan 14 [PMID: 19132776] (WB, Human) WB Human
Flint M, Maidens C, Loomis-Price LD et al. Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81. J Virol. 1999 Aug [PMID: 10400713] (ELISA, WB, Human) ELISA, WB Human
Ji Y, Qi D, Li L et al. Multiplexed Profiling of Single-cell Extracellular Vesicles Secretion. Carbohydrate Polymers. (ICC/IF, Mouse) ICC/IF Mouse
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. Feb 8 2019 [PMID: 30736829] (WB, Human) WB Human
Shin H, Jeong H, Park J et al. Correlation between Cancerous Exosomes and Protein Markers Based on Surface-Enhanced Raman Spectroscopy (SERS) and Principal Component Analysis (PCA). ACS Sens. Nov 9 2018 [PMID: 30381940] (WB, Human) WB Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD81 Antibody (NB100-65805) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65805AF405
Alexa Fluor 488 Labeled NB100-65805AF488
Alexa Fluor 647 Labeled NB100-65805AF647
Alexa Fluor 700 Labeled NB100-65805AF700
Allophycocyanin Labeled NB100-65805APC
DyLight 350 Labeled NB100-65805UV
DyLight 405 Labeled NB100-65805V
DyLight 488 Labeled NB100-65805G
DyLight 680 Labeled NB100-65805FR
DyLight 755 Labeled NB100-65805IR
FITC Labeled NB100-64060
PE Labeled NB100-65805PE
PerCP Labeled NB100-65805PCP

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Bioinformatics Tool for CD81 Antibody (NB100-65805)

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Blogs on CD81.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

CD81/TAPA1: I'm on Tapa the Cell
Target of the antiproliferative 1 (TAPA1), also known as CD81, is found in the plasma membrane in lymphocytes and plays an important role in the regulation of lymphoma cell growth. This transmembrane 4 superfamily (TM4SF) protein is primarily found on...  Read full blog post.

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Bioinformatics

Gene Symbol CD81