CD45 Antibody (OX-1) [DyLight 350]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
OX-1
Clonality
Monoclonal
Host
Mouse

CD45 Antibody (OX-1) [DyLight 350] Summary

Immunogen
A 100kD thymocyte glycoprotein fraction enriched for the rat leukocyte common antigen (L-CA).
Localization
Type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 350
Format
DyLight 350 (A=353, E=432)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD45 Antibody (OX-1) [DyLight 350]

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC 3.1.3.48
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD45 Antibody (NB100-64895UV). (Showing 1 - 2 of 2 FAQ).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-64895APC
DyLight 405 Labeled NB100-64895V
DyLight 405LS Labeled NB100-64895V3
DyLight 488 Labeled NB100-64895G
DyLight 680 Labeled NB100-64895FR
DyLight 755 Labeled NB100-64895IR
PE Labeled NB100-64895PE
PerCP Labeled NB100-64895PCP

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Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol PTPRC
Entrez
Uniprot

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