CD4 Antibody (RPA-T4)

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Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative ...read more
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Cell surface flow analysis of CD4 in human PBMC using this antibody at 0.25 ug/10^6 cells. Cells were stained with primary antibody followed by a PE-conjugated goat ...read more
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using Azide Free version of NBP2-25199. CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red ...read more
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium ...read more
Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, In vitro, B/N, IHC-P (-)
Clone
RPA-T4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CD4 Antibody (RPA-T4) Summary

Immunogen
PHA-stimulated human PBMC.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • In vitro assay
  • Block/Neutralize
Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.. Use in in vitro reported in multiple pieces scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25199 in the following applications:

Publications
Read Publications using
NBP2-25199 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CD4 Antibody (RPA-T4)

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (NBP2-25199)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, IHC-Fr, In vitro.


Filter By Application
FLOW
(5)
IHC-Fr
(1)
In vitro
(3)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-25199 Applications Species
Hellwinkel JE, Redzic JS, Harland TA et al. Glioma-derived extracellular vesicles selectively suppress immune responses. Neuro-oncology. 2016 Apr [PMID: 26385614] (FLOW, Human)

Details:
This reference used the Azide Free version of NBP2-25199.
FLOW Human
Hellwinkel JE, Redzic JS, Harland TA et al. Glioma-derived extracellular vesicles selectively suppress immune responses Neuro-oncology 2016 Apr [PMID: 26385614] (FLOW, Human)

Details:
This reference used the Azide Free version of NBP2-25199.
FLOW Human
Kaneko H, Saito K, Hashimoto H et al. Preferential elimination of CD28+ T cells in systemic lupus erythematosus (SLE) and the relation with activation-induced apoptosis Clin Exp Immunol. 1996 Nov [PMID: 8918566]
Schols D, De Clercq E. Human immunodeficiency virus type 1 gp120 induces anergy in human peripheral blood lymphocytes by inducing interleukin-10 production J Virol. 1996 Aug [PMID: 8764000] (In vitro, Human) In vitro Human
Res P, Martinez-Caceres E, Cristina Jaleco A et al. CD34+CD38dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells Blood. 1996 Jun 15 [PMID: 8652833] (FLOW, Human) FLOW Human
Ruggiero G, Martinez Caceres E, Voordouw A et al. CD40 expressed on thymic epithelial cells provides costimulation for proliferation but not for apoptosis of human thymocytes J Immunol. 1996 May 15 [PMID: 8621909]
Chabanne L, Marchal T, Kaplanski C et al. Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes Tissue Antigens. 1994 Mar 1 [PMID: 8091419]
Deng MC, Bell S, Huie P et al. Cardiac allograft vascular disease Relationship to microvascular cell surface markers and inflammatory cell phenotypes on endomyocardial biopsy Circulation. 1995 Mar 15 [PMID: 7882470] (IHC-Fr, Human) IHC-Fr Human
Barcena A, Muench MO, Galy AH et al. Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid-cell development Blood. 1993 Dec 1 [PMID: 7694684] (FLOW, Human) FLOW Human
Galy A, Verma S, Barcena A, Spits H. Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34 Delineation of early events in human thymic development J Exp Med. 1993 Aug 1 [PMID: 7688021] (FLOW, In vitro, Human) FLOW, In vitro Human
Show All 13 Publications.

Review for CD4 Antibody (NBP2-25199) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-25199:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Sachin Puri
ICC Human 02/28/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedTonsils,
SpeciesHuman

Blocking

Blocking Detailshorse blocking serum

Primary Anitbody

Dilution Ratioanti CD4

Secondary Antibody

Secondary Descriptionanti rabbit IgG

Details

Detection NotesDAB
Fixation DetailsAntigen Retrival using microwave

Product General Protocols

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FAQs for CD4 Antibody (NBP2-25199). (Showing 1 - 1 of 1 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-27245
DyLight 350 Labeled NBP2-27216UV
DyLight 405 Labeled NBP2-27216V
DyLight 488 Labeled NBP2-27216G
DyLight 550 Labeled NBP2-27216R
DyLight 650 Labeled NBP2-27216C
DyLight 680 Labeled NBP2-27216FR
DyLight 755 Labeled NBP2-27216IR
HRP Labeled NBP2-27216H
PE Labeled NBP2-27248
PerCP Labeled NBP2-27216PCP

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Bioinformatics Tool for CD4 Antibody (NBP2-25199)

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Blogs on CD4. Showing 1-10 of 16 blog posts - Show all blog posts.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

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Sachin Puri
02/28/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CD4
Uniprot