HLA ABC Antibody (W6/32) - Azide and BSA Free

Images

 
Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary ...read more
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue ...read more
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Pm, PmSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, IHC-P (-)
Clone
W6/32
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

HLA ABC Antibody (W6/32) - Azide and BSA Free Summary

Immunogen
Purified human tonsil lymphocyte membranes.
Specificity
NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody does not work in Immunohistochemistry-Paraffin.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-64775 in the following applications:

Publications
Read Publications using
NB100-64775 in the following applications:

  • B/N
    1 publication
  • 1 publication
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • MCS
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (Parasar P et al).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HLA ABC Antibody (W6/32) - Azide and BSA Free

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLAA
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA ABC Antibody (NB100-64775)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: B/N, ELISA, IHC-Fr, IHC-P, IP, MCS, WB.


Filter By Application
B/N
(1)
ELISA
(1)
IHC-Fr
(1)
IHC-P
(1)
IP
(1)
MCS
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-64775 Applications Species
Yanagisawa M, Nagamatsu T, Kurano M et al. Upregulation of autotaxin by oxidative stress via Nrf2 activation: a novel insight into the compensation mechanism in preeclamptic placenta Journal of Reproductive Immunology 2023-09-01 [PMID: 37837670]
Phulphagar KM, Ctortecka C, Jacome ASV et al. Sensitive, High-Throughput HLA-I and HLA-II Immunopeptidomics Using Parallel Accumulation-Serial Fragmentation Mass Spectrometry Molecular & Cellular Proteomics 2023-06-01 [PMID: 37142057]
Phulphagar KM, Ctortecka C, Vaca Jacome AS et al. Sensitive, high-throughput HLA-I and HLA-II immunopeptidomics using parallel accumulation-serial fragmentation mass spectrometry bioRxiv : the preprint server for biology 2023-03-18 [PMID: 36993564] (IP, Human) IP Human
Mimura N, Nagamatsu T, Morita K et al. Suppression of human trophoblast syncytialization by human cytomegalovirus infection Placenta 2021-12-11 [PMID: 34933151] (MCS, Human) MCS Human
JGM Strijker, R Pscheid, E Drent, JJF van der Ho, B Koopmans, K Ober, SR van Hooff, WM Kholosy, AM Cornel, C Coomans, A Bisso, MM van Loenen, JJ Molenaar, J Wienke alphabeta-T Cells Engineered to Express gammadelta-T Cell Receptors Can Kill Neuroblastoma Organoids Independent of MHC-I Expression Journal of personalized medicine, 2021-09-17;11(9):. 2021-09-17 [PMID: 34575700] (B/N) B/N
Javeed N, Her TK, Brown MR Et al. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell reports 2021-08-24 [PMID: 34433033] (IHC-P, Mouse) IHC-P Mouse
Darwish M, Wichner S, Li J et al. High-throughput identification of conditional MHCI ligands and scaled-up production of conditional MHCI complexes Protein science : a publication of the Protein Society 2021-04-11 [PMID: 33840137]
Wang Y, Huang J et al. The Plasticity of Mesenchymal Stem Cells in Regulating Surface HLA-I. iScience 2019-05-31 [PMID: 31030183] (WB, Human) WB Human
Jones, DC et al. 2011 HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding. J Immunol 186: 2990-7. 2011-01-01 [PMID: 21270408]
Zuo, J et al. The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways. J Virol 85: 1604-14. 2011-01-01 [PMID: 21123379]
Show All 29 Publications.

Reviews for HLA ABC Antibody (NB100-64775) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-64775:
Filter by Applications
IHC-Fr
(1)
Flow
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen HLA ABC NB100-64775
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 01/31/2022
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedColon cancer
SpeciesMouse
LotW6/32

Comments

CommentsPrimary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution
Flow Cytometry HLA ABC NB100-64775
Enlarge
4
reviewed by:
Verified Customer
Flow Human 02/28/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedCell Line, lymphoblast
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for HLA ABC Antibody (NB100-64775). (Showing 1 - 1 of 1 FAQs).

  1. We have purchased the antibody NB100-64775 HLA ABC Antibody (W6/32) 0.25mg. To date we have completed over 40 reactions and haven't found a single positive output. Can you please send me the flow cytometry images generated by this antibody??
    • I'm sorry to hear about the dificulty you have been having. Please referĀ to the images section of the datasheet. There are also many publicationsĀ on the product page for your review. I did find PubMeb 21123379 links to a free PMC article that talks about this clone being used in Flow Cytometry. We also offer many conjugated forms of this antibody that you may be interested in. You can always contact technical@novusbio.com with any additional questions.

Secondary Antibodies

 

Isotype Controls

Additional HLA ABC Products

Array NB100-64775

Research Areas for HLA ABC Antibody (NB100-64775)

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Blogs on HLA ABC

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
01/31/2022
Application: IHC-Fr
Species: Mouse

Verified Customer
02/28/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol HLA-A