HLA ABC Antibody (W6/32)

Images

 
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue ...read more
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.
Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue ...read more

Product Details

Summary
Reactivity Hu, Bb, Bv, PmSpecies Glossary
Applications ELISA, Flow, Func, ICC/IF, IHC, IHC-Fr, IP
Clone
W6/32
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HLA ABC Antibody (W6/32) Summary

Immunogen
Purified human tonsil lymphocyte membranes.
Specificity
NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody does not work in Immunohistochemistry-Paraffin.
Reviewed Applications
Read 1 Review rated 4
using
NB100-64775 in the following applications:

Publications
Read Publications using NB100-64775.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HLA ABC Antibody (W6/32)

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLAA
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA ABC Antibody (NB100-64775)(22)

We have publications tested in 1 application: IHC-Fr.


Filter By Application
IHC-Fr
(2)
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All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-64775 Applications Species
Jones, DC et al. 2011 HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding. J Immunol 186: 2990-7. 2011 [PMID: 21270408]
Zuo, J et al. The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways. J Virol 85: 1604-14. 2011 [PMID: 21123379]
Fujita, Y et al. Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice. Proc Natl Acad Sci U S A 107: 14345-50. 2010 [PMID: 20660747]
Narita, M et al. WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase. Int J Med Sci 7: 72-81. 2010 [PMID: 20428337]
Vitadello, M et al. Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation. Arthritis Res Ther 12: R52. 2010 [PMID: 20334640]
Hinrichs, J et al. The nature of peptides presented by an HLA class I low expression allele. Haematologica 95: 1373-80. 2010 [PMID: 20220067]
Spentzou, A et al. Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates. J Infect Dis 201: 720-9. 2010 [PMID: 20132004]
Grotzke, JE et al. The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle. PLoS Pathog 5: e1000374. 2009 [PMID: 19360129]
Koene G, Arts-Hilkes et al. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss Hum Immunol 2004 [PMID: 15603873]

Details:
This citation used the FITC version of this antibody.
Dressel, R et al. Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes. Cancer Res 63: 8212-8220. 2003 [PMID: 14678977]
Show All 22 Publications.

Review for HLA ABC Antibody (NB100-64775) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB100-64775:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry HLA ABC NB100-64775
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reviewed by:
Anonymous
Flow Human 02/28/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedCell Line, lymphoblast
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for HLA ABC Antibody (NB100-64775). (Showing 1 - 1 of 1 FAQs).

  1. We have purchased the antibody http://www.novusbio.com/HLA-ABC-Antibody-W6-32_NB100-64775.html. HLA ABC Antibody (W6/32) 0.25mg. To date we have completed over 40 reactions and haven't found a single positive output. Can you please send me the flow cytometry images generated by this antibody??
    • I'm sorry to hear about the dificulty you have been having. Please see this link to the images section of the datasheet. There are also many publications for your review. I did find PubMeb 21123379 links to a free PMC article that talks about this clone being used in Flow Cytometry. We also offer many conjugated forms of this antibody that you may be interested in. You can always contact technical@novusbio.com with any additional questions.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64775AF405
Alexa Fluor 488 Labeled NB100-64775AF488
Alexa Fluor 647 Labeled NB100-64775AF647
Alexa Fluor 700 Labeled NB100-64775AF700
Allophycocyanin Labeled NB100-64775APC
DyLight 350 Labeled NB100-64775UV
DyLight 405 Labeled NB100-64775V
DyLight 488 Labeled NB100-64775G
DyLight 680 Labeled NB100-64775FR
DyLight 755 Labeled NB100-64775IR
HRP Labeled NB100-64625
PE Labeled NB100-64775PE
PerCP Labeled NB100-64775PCP

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HLA ABC NB100-64775

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Recent Reviews

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1
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Anonymous
02/28/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol HLA-A