B7-2/CD86 Antibody (BU63) [Allophycocyanin]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP
Clone
BU63
Clonality
Monoclonal
Host
Mouse

B7-2/CD86 Antibody (BU63) [Allophycocyanin] Summary

Immunogen
ARH-77 (B-lymphoblastoid cell line)
Localization
Cell Surface
Marker
DC Maturation Marker
Specificity
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD86
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Allophycocyanin
Format
Allophycocyanin (A=620-650, E=660-670)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for B7-2/CD86 Antibody (BU63) [Allophycocyanin]

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B7-2/CD86
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B7-2/CD86 Antibody (NBP2-34569APC) (0)

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Secondary Antibodies

 

Isotype Controls

Additional B7-2/CD86 Products

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Bioinformatics Tool for B7-2/CD86 Antibody (NBP2-34569APC)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (NBP2-34569APC). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-2/CD86 Antibody (NBP2-34569APC)

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Research Areas for B7-2/CD86 Antibody (NBP2-34569APC)

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Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CD86
Entrez
Uniprot

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