Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.

Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Po, V-Vi
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
• Clone: HSn 7D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free Summary

• Immunogen: Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
• Epitope: Within domains 1-4.
• Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • In vitro assay
  • Radioimmunoassay
  • Western Blot
• Application Notes: This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature (PMID: 18414664). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28794041). Use in Immunohistochemistry reported in scientific literature (PMID: 26106049).

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). . Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

• CD169
• FLJ00051
• sialic acid binding Ig-like lectin 1, sialoadhesin
• sialoadhesin
• Siglec1
• Siglec-1

Background

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-1/CD169 Antibody (NB600-534)(20)

Publications using NB600-534

• Tanboon J, Uruha A, Arahata Y et al. Inflammatory features in sporadic late-onset nemaline myopathy are independent from monoclonal gammopathy Brain pathology (Zurich, Switzerland) 2021-05-01 [PMID: 34043258]
• Ostendorf L, Dittert P, Biesen R et al. SIGLEC1 (CD169): a marker of active neuroinflammation in the brain but not in the blood of multiple sclerosis patients Scientific reports 2021-05-13 [PMID: 33986412] (IHC, Human)
• Verollet C, Dupont M, Souriant S et al. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages Elife 2020-04-01 [PMID: 32223897]
• Roos A, Preusse C, Hathazi D et al. Proteomic Profiling Unravels a Key Role of Specific Macrophage Subtypes in Sporadic Inclusion Body Myositis Front Immunol 2019-05-31 [PMID: 31143183] (IHC-Fr, Human)
• OhAinle M, Helms L, Vermeire J et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV Elife 2018-12-06 [PMID: 30520725] (FLOW, Human)
• Bihari C, Lal D, Thakur M et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. Hepatol Commun 2018-09-01 [PMID: 30202823] (IHC, Human)
• Jadapalli JK, Wright GW, Kain V et al. Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters the inflammation-resolution program in the myocardium Am J Physiol Heart Circ Physiol 2018-08-04 [PMID: 30074834] (IHC-Fr, Mouse)
• Akiyama H, Ramirez NP, Gibson G et al. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon J Virol 2017-08-11 [PMID: 28794041] (IHC-Fr, Human)
• de Carvalho LM, Ngoumou G, Park JW et al. Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy Arthritis & rheumatology (Hoboken, N.J.) 2017-10-01 [PMID: 28605144] (IHC-Fr, Human)
• Novakovic P, Harding JC, Ladinig A et al. Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus. Vet. Res. 2016-08-06 [PMID: 27494990] (IHC, Porcine)
• Yayi H, Yeda X, Huaxin W et al. Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats. Acta Cir Bras. 2016-07-01 [PMID: 27487279] (WB, Virus)
• Groenendyk J, Lee D, Jung J et al. Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis PLoS ONE 2016-07-22 [PMID: 27441395] (IHC, Porcine)
• Kusumi S, Koga D, Kanda TUshiki T. Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen. Biomed Res 2015-06-24 [PMID: 26106049] (IHC, Human)
  
  Details:
  Using the PE conjugated version of NB600-534, catalog number NB600-534PE.
• Verollet C, Dupont M, Souriant S et al. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages BioRxiv (ICC/IF, IHC-P, Human)
• Akiyama H, Ramirez NG, Gudheti MV, Gummuluru S. CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog. 2015-03-01 [PMID: 25760631] (FLOW, WB, Human)
• Puryear WB, Akiyama H, Geer SD et al. Interferon-Inducible Mechanism of Dendritic Cell-Mediated HIV-1 Dissemination Is Dependent on Siglec-1/CD169. PLoS Pathog 2013-04-01 [PMID: 23593001] (WB, Human)
• Rempel H, Calosing C, Sun B Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS One. 2008-04-16 [PMID: 18414664] (FLOW, In vitro, WB, Human)
• Pack M, Trumpfheller C, Thomas D et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2008-03-01 [PMID: 17944899] (IHC-Fr, Human)
• Hartnell A, Steel J, Turley H et al. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood;97(1):288-96. 2001-01-01 [PMID: 11133773] (IHC)
• Steiniger B et al. The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone. Immunol 92: 307-316. 1997-01-01 [PMID: 9415041]

Review for Siglec-1/CD169 Antibody (NB600-534) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: David Sun IHC-Fr Mouse 12/16/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult small intestine
• Species: Mouse
• Lot: A-5

Bioinformatics

• Gene Symbol: SIGLEC1
• Entrez:
  • 6614(Human)
• Uniprot:
  • Q9BZZ2(Human)