Siglec-1/CD169 Antibody (HSn 7D2)

Images

 
Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI ...read more
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled ...read more
Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Po, V-ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
Clone
HSn 7D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Siglec-1/CD169 Antibody (HSn 7D2) Summary

Immunogen
Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
Epitope
Within domains 1-4.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • In vitro assay
  • Radioimmunoassay
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature (PMID: 18414664). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28794041). Use in Immunohistochemistry reported in scientific literature (PMID: 26106049).
Reviewed Applications
Read 1 Review rated 5
using
NB600-534 in the following applications:

Publications
Read Publications using
NB600-534 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Virus reactivity reported in scientific literature (PMID: 27487279). Mouse reactivity reported in scientific literature. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2)

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-1/CD169 Antibody (NB600-534)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Porcine, Virus.

We have publications tested in 8 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB, in vitro.


Filter By Application
FLOW
(2)
Flow
(1)
ICC/IF
(4)
IHC
(6)
IHC-Fr
(2)
IHC-P
(1)
WB
(4)
in vitro
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
Porcine
(2)
Virus
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB600-534 Applications Species
Roos A, Preusse C, Hathazi D, et al. Proteomic Profiling Unravels a Key Role of Specific Macrophage Subtypes in Sporadic Inclusion Body Myositis Front Immunol May 9 2019 [PMID: 31143183] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Bihari C, Lal D, Thakur M et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. Hepatol Commun Sep 1 2018 [PMID: 30202823] (IHC, Human) IHC Human
Akiyama H, Ramirez NP, Gibson G et al. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon J. Virol. 2017 Aug 09 [PMID: 28794041] (ICC/IF, Human) ICC/IF Human
Verollet C, Dupont M, Souriant S et al. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages BioRxiv (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human
OhAinle M, Helms L, Vermeire J et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV Elife Dec 6 2018 [PMID: 30520725] (Flow, Human) Flow Human
Jadapalli JK, Wright GM, Kain V et al. Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters inflammation-resolution program in the myocardium Am. J. Physiol. Heart Circ. Physiol. Aug 3 2018 [PMID: 30074834] (ICC/IF, Mouse) ICC/IF Mouse
de Carvalho LM, Ngoumou G, Park JW et al. Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy Arthritis & rheumatology (Hoboken, N.J.) Oct 1 2017 [PMID: 28605144] (IHC-Fr, Human) IHC-Fr Human
Novakovic P, Harding JC, Ladinig A et al. Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus. Vet. Res. Aug 6 2016 [PMID: 27494990] (IHC, Porcine) IHC Porcine
Yayi H, Yeda X, Huaxin W et al. Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats. Acta Cir Bras. Jul 1 2016 [PMID: 27487279] (WB, Virus) WB Virus
Groenendyk J, Lee D, Jung J et al. Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis PLoS ONE 2016 Jul 22 [PMID: 27441395] (IHC, Porcine) IHC Porcine
Show All 17 Publications.

Review for Siglec-1/CD169 Antibody (NB600-534) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-534:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Siglec-1/CD169 NB600-534
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 12/16/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult small intestine
SpeciesMouse
LotA-5

Product General Protocols

View specific protocols for Siglec-1/CD169 Antibody (NB600-534): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Siglec-1/CD169 Antibody (NB600-534) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-534AF350
Alexa Fluor 405 NB600-534AF405
Alexa Fluor 488 NB600-534AF488
Alexa Fluor 532 NB600-534AF532
Alexa Fluor 594 NB600-534AF594
Alexa Fluor 647 NB600-534AF647
Alexa Fluor 700 NB600-534AF700
Alexa Fluor 750 NB600-534AF750
Allophycocyanin NB600-534APC
Allophycocyanin/Cy7 NB600-534APCCY7
Biotin NB600-534B
DyLight 350 NB600-534UV
DyLight 405 NB600-534V
DyLight 488 NB600-534G
DyLight 550 NB600-534R
DyLight 594 NB600-534DL594
DyLight 650 NB600-534C
DyLight 680 NB600-534FR
DyLight 755 NB600-534IR
FITC NB600-534F
HRP NB600-534H
Janelia Fluor 549 NB600-534JF549
Janelia Fluor 646 NB600-534JF646
PE NB600-534PE
PE/Atto594 NB600-534PEATT594
PE/Cy5.5 NB600-534PECY55
PE/Cy7 NB600-534PECY7
PerCP NB600-534PCP

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
12/16/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol SIGLEC1
Entrez
Uniprot