Siglec-1/CD169 Antibody (HSn 7D2)

Images

 
Immunohistochemistry-Paraffin: Sialoadhesin Antibody (HSn 7D2) [NB600-534] - IHC staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti- Siglec-1/CD169 Monoclonal Antibody (Catalog # NB600-534, ...read more

Product Details

Summary
Reactivity Hu, Po, ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
Clone
HSn 7D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Siglec-1/CD169 Antibody (HSn 7D2) Summary

Immunogen
Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
Epitope
Within domains 1-4.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • In vitro assay
  • Radioimmunoassay
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature ( PMID 18414664).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28794041).
Publications
Read Publications using
NB600-534 in the following applications:

Reactivity Notes

Human. Porcine reactivity reported in scientific literature (PMID: 27441395). Virus reactivity reported in scientific literature (PMID: 27487279). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2)

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin

Background

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-1/CD169 Antibody (NB600-534)(11)

We have publications tested in 3 confirmed species: Human, Porcine, Virus.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, WB, in vitro.


Filter By Application
FLOW
(2)
ICC/IF
(1)
IHC
(4)
IHC-Fr
(1)
WB
(4)
in vitro
(1)
All Applications
Filter By Species
Human
(6)
Porcine
(2)
Virus
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB600-534 Applications Species
Akiyama H, Ramirez NP, Gibson G et al. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon J. Virol. 2017 Aug 09 [PMID: 28794041] (ICC/IF, Human) ICC/IF Human
Novakovic P, Harding JC, Ladinig A et al. Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus. Vet. Res. Aug 6 2016 [PMID: 27494990] (IHC, Porcine) IHC Porcine
Yayi H, Yeda X, Huaxin W et al. Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats. Acta Cir Bras. Jul 1 2016 [PMID: 27487279] (WB, Virus) WB Virus
Groenendyk J, Lee D, Jung J et al. Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis PLoS ONE 2016 Jul 22 [PMID: 27441395] (IHC, Porcine) IHC Porcine
Kusumi S, Koga D, Kanda TUshiki T. Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen. Biomed Res 2015 Jun 24 [PMID: 26106049] (IHC, Human)

Details:
Using the PE conjugated version of NB600-534, catalog number NB600-534PE.
IHC Human
Akiyama H, Ramirez NG, Gudheti MV, Gummuluru S. CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog. 2015 Mar 01 [PMID: 25760631] (WB, FLOW, Human) WB, FLOW Human
Puryear WB, Akiyama H, Geer SD et al. Interferon-Inducible Mechanism of Dendritic Cell-Mediated HIV-1 Dissemination Is Dependent on Siglec-1/CD169. PLoS Pathog 2013 Apr [PMID: 23593001] (WB, Human) WB Human
Rempel H, Calosing C, Sun B Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS One. 2008 Apr 16 [PMID: 18414664] (FLOW, WB, in vitro, Human) FLOW, WB, in vitro Human
Pack M, Trumpfheller C, Thomas D et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2008 Mar [PMID: 17944899] (IHC-Fr, Human) IHC-Fr Human
Hartnell A, Steel J, Turley H et al. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood;97(1):288-96. 2001 Jan 1. [PMID: 11133773] (IHC) IHC
Show All 11 Publications.

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Product General Protocols

View specific protocols for Siglec-1/CD169 Antibody (NB600-534): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Siglec-1/CD169 Antibody (NB600-534) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-534AF405
Alexa Fluor 488 Labeled NB600-534AF488
Alexa Fluor 647 Labeled NB600-534AF647
Alexa Fluor 700 Labeled NB600-534AF700
Allophycocyanin Labeled NB600-534APC
DyLight 350 Labeled NB600-534UV
DyLight 405 Labeled NB600-534V
DyLight 488 Labeled NB600-534G
DyLight 680 Labeled NB600-534FR
DyLight 755 Labeled NB600-534IR
FITC Labeled NB600-534F
PE Labeled NB600-534PE
PerCP Labeled NB600-534PCP

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Bioinformatics

Gene Symbol SIGLEC1
Entrez
Uniprot