Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Images

 
Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI ...read more
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled ...read more
Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Po, V-ViSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC
Clone
HSn 7D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free Summary

Immunogen
Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
Epitope
Within domains 1-4.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 28794041)
  • Immunohistochemistry 1:10-1:500. Use reported in scientific literature (PMID 26106049)
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • In vitro assay
  • In vivo assay reported in scientific literature (PMID 18414664)
  • Radioimmunoassay
  • Western Blot reported in scientific literature
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA.
Reviewed Applications
Read 1 Review rated 5
using
NB600-534 in the following applications:

Publications
Read Publications using
NB600-534 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-1/CD169 Antibody (NB600-534)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Porcine, Virus.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, In vitro, WB.


Filter By Application
FLOW
(4)
ICC/IF
(1)
IF/IHC
(6)
IHC-Fr
(5)
IHC-P
(1)
In vitro
(1)
WB
(4)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
Porcine
(2)
Virus
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB600-534 Applications Species
Zwart ES, van Ee T, Affandi AJ et al. Spatial immune composition of tumor microenvironment in patients with pancreatic cancer Cancer immunology, immunotherapy : CII 2023-11-08 [PMID: 37938368] (FLOW, Human) FLOW Human
Tanboon J, Uruha A, Arahata Y et al. Inflammatory features in sporadic late-onset nemaline myopathy are independent from monoclonal gammopathy Brain pathology (Zurich, Switzerland) 2021-05-01 [PMID: 34043258]
Ostendorf L, Dittert P, Biesen R et al. SIGLEC1 (CD169): a marker of active neuroinflammation in the brain but not in the blood of multiple sclerosis patients Scientific reports 2021-05-13 [PMID: 33986412] (IF/IHC, Human) IF/IHC Human
Verollet C, Dupont M, Souriant S et al. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages Elife 2020-04-01 [PMID: 32223897]
Roos A, Preusse C, Hathazi D et al. Proteomic Profiling Unravels a Key Role of Specific Macrophage Subtypes in Sporadic Inclusion Body Myositis Front Immunol 2019-05-31 [PMID: 31143183] (IHC-Fr, Human) IHC-Fr Human
OhAinle M, Helms L, Vermeire J et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV Elife 2018-12-06 [PMID: 30520725] (FLOW, Human) FLOW Human
Bihari C, Lal D, Thakur M et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. Hepatol Commun 2018-09-01 [PMID: 30202823] (IF/IHC, Human) IF/IHC Human
Jadapalli JK, Wright GW, Kain V et al. Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters the inflammation-resolution program in the myocardium Am J Physiol Heart Circ Physiol 2018-08-04 [PMID: 30074834] (IHC-Fr, Mouse) IHC-Fr Mouse
Akiyama H, Ramirez NP, Gibson G et al. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon J Virol 2017-08-11 [PMID: 28794041] (IHC-Fr, Human) IHC-Fr Human
de Carvalho LM, Ngoumou G, Park JW et al. Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy Arthritis & rheumatology (Hoboken, N.J.) 2017-10-01 [PMID: 28605144] (IHC-Fr, Human) IHC-Fr Human
Show All 21 Publications.

Review for Siglec-1/CD169 Antibody (NB600-534) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-534:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Siglec-1/CD169 NB600-534
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 12/16/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult small intestine
SpeciesMouse
LotA-5

Product General Protocols

View specific protocols for Siglec-1/CD169 Antibody (NB600-534): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Siglec-1/CD169 Antibody (NB600-534) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
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1
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Verified Customer
12/16/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol SIGLEC1
Entrez
Uniprot