Siglec-1/CD169 Antibody (HSn 7D2)


Immunohistochemistry-Paraffin: Sialoadhesin Antibody (HSn 7D2) [NB600-534] - IHC staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti- Siglec-1/CD169 Monoclonal Antibody (Catalog # NB600-534, more

Product Details

Reactivity Hu, Po, ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
HSn 7D2
1.0 mg/ml

Siglec-1/CD169 Antibody (HSn 7D2) Summary

Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
Within domains 1-4.
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • In vitro assay
  • Radioimmunoassay
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA. Western Blot was reported in scientific literature. Use in in vitro assay reported in scientific literature ( PMID 18414664).Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28794041).
Read Publications using
NB600-534 in the following applications:

Reactivity Notes

Human. Porcine reactivity reported in scientific literature (PMID: 27441395). Virus reactivity reported in scientific literature (PMID: 27487279). Not yet tested in other species.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2)

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin


Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ELISA, ICC/IF
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Po, Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA

Publications for Siglec-1/CD169 Antibody (NB600-534)(13)

We have publications tested in 4 confirmed species: Human, Mouse, Porcine, Virus.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, WB, in vitro.

Filter By Application
in vitro
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-534 Applications Species
Jadapalli JK, Wright GM, Kain V et al. Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters inflammation-resolution program in the myocardium Am. J. Physiol. Heart Circ. Physiol. Aug 3 2018 [PMID: 30074834] (ICC/IF, Mouse) ICC/IF Mouse
Akiyama H, Ramirez NP, Gibson G et al. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon J. Virol. 2017 Aug 09 [PMID: 28794041] (ICC/IF, Human) ICC/IF Human
de Carvalho LM, Ngoumou G, Park JW et al. Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy Arthritis & rheumatology (Hoboken, N.J.) Oct 1 2017 [PMID: 28605144] (IHC-Fr, Human) IHC-Fr Human
Novakovic P, Harding JC, Ladinig A et al. Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus. Vet. Res. Aug 6 2016 [PMID: 27494990] (IHC, Porcine) IHC Porcine
Yayi H, Yeda X, Huaxin W et al. Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats. Acta Cir Bras. Jul 1 2016 [PMID: 27487279] (WB, Virus) WB Virus
Groenendyk J, Lee D, Jung J et al. Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis PLoS ONE 2016 Jul 22 [PMID: 27441395] (IHC, Porcine) IHC Porcine
Kusumi S, Koga D, Kanda TUshiki T. Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen. Biomed Res 2015 Jun 24 [PMID: 26106049] (IHC, Human)

Using the PE conjugated version of NB600-534, catalog number NB600-534PE.
IHC Human
Akiyama H, Ramirez NG, Gudheti MV, Gummuluru S. CD169-Mediated Trafficking of HIV to Plasma Membrane Invaginations in Dendritic Cells Attenuates Efficacy of Anti-gp120 Broadly Neutralizing Antibodies. PLoS Pathog. 2015 Mar 01 [PMID: 25760631] (WB, FLOW, Human) WB, FLOW Human
Puryear WB, Akiyama H, Geer SD et al. Interferon-Inducible Mechanism of Dendritic Cell-Mediated HIV-1 Dissemination Is Dependent on Siglec-1/CD169. PLoS Pathog 2013 Apr [PMID: 23593001] (WB, Human) WB Human
Rempel H, Calosing C, Sun B Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity. PLoS One. 2008 Apr 16 [PMID: 18414664] (FLOW, WB, in vitro, Human) FLOW, WB, in vitro Human
Show All 13 Publications.

Reviews for Siglec-1/CD169 Antibody (NB600-534) (0)

There are no reviews for Siglec-1/CD169 Antibody (NB600-534). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for Siglec-1/CD169 Antibody (NB600-534): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Siglec-1/CD169 Antibody (NB600-534) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-534AF405
Alexa Fluor 488 Labeled NB600-534AF488
Alexa Fluor 647 Labeled NB600-534AF647
Alexa Fluor 700 Labeled NB600-534AF700
Allophycocyanin Labeled NB600-534APC
DyLight 350 Labeled NB600-534UV
DyLight 405 Labeled NB600-534V
DyLight 488 Labeled NB600-534G
DyLight 680 Labeled NB600-534FR
DyLight 755 Labeled NB600-534IR
FITC Labeled NB600-534F
PE Labeled NB600-534PE
PerCP Labeled NB600-534PCP

Additional Array Products

Bioinformatics Tool for Siglec-1/CD169 Antibody (NB600-534)

Discover related pathways, diseases and genes to Siglec-1/CD169 Antibody (NB600-534). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Siglec-1/CD169 Antibody (NB600-534)

Discover more about diseases related to Siglec-1/CD169 Antibody (NB600-534).

Pathways for Siglec-1/CD169 Antibody (NB600-534)

View related products by pathway.

PTMs for Siglec-1/CD169 Antibody (NB600-534)

Learn more about PTMs related to Siglec-1/CD169 Antibody (NB600-534).

Research Areas for Siglec-1/CD169 Antibody (NB600-534)

Find related products by research area.

Blogs on Siglec-1/CD169

There are no specific blogs for Siglec-1/CD169, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Siglec-1/CD169 Antibody (HSn 7D2) and receive a gift card or discount.


Gene Symbol SIGLEC1