CD4 Antibody (RPA-T4) [DyLight 405]

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Flow Cytometry: CD4 Antibody (RPA-T4) [DyLight 405] [NBP2-27216V] - analysis of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, B/N, IHC-P (-)
Clone
RPA-T4
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

CD4 Antibody (RPA-T4) [DyLight 405] Summary

Immunogen
PHA-stimulated human PBMC.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Block/Neutralize
Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-27216V in the following applications:

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD4 Antibody (RPA-T4) [DyLight 405]

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (NBP2-27216V) (0)

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Review for CD4 Antibody (NBP2-27216V) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-27216V:
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD4 NBP2-27216V
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reviewed by:
Anonymous
Flow Human 06/30/2015
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Summary

ApplicationFlow Cytometry
Sample TestedPBMCs
SpeciesHuman

Blocking

Blocking Detailsnone

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD4 Antibody (NBP2-27216V). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-27245
DyLight 350 Labeled NBP2-27216UV
DyLight 488 Labeled NBP2-27216G
DyLight 550 Labeled NBP2-27216R
DyLight 650 Labeled NBP2-27216C
DyLight 680 Labeled NBP2-27216FR
DyLight 755 Labeled NBP2-27216IR
HRP Labeled NBP2-27216H
PE Labeled NBP2-27248
PerCP Labeled NBP2-27216PCP

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Blogs on CD4. Showing 1-10 of 17 blog posts - Show all blog posts.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Showing 1-10 of 17 blog posts - Show all blog posts.

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Recent Reviews

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Anonymous
06/30/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD4