Siglec-1/CD169 Antibody (HSn 7D2) [Alexa Fluor® 488]

Product Image: Siglec-1/CD169 Antibody (HSn 7D2) [Alexa Fluor® 488] [NB600-534AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

• Reactivity: Hu, Mu, Po, V-Vi
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC
• Clone: HSn 7D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Siglec-1/CD169 Antibody (HSn 7D2) [Alexa Fluor® 488] Summary

• Immunogen: Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]
• Epitope: Within domains 1-4.
• Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In vitro assay
  • In vivo assay
  • Radioimmunoassay
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Siglec-1/CD169 Antibody (HSn 7D2) [Alexa Fluor® 488]

• CD169
• FLJ00051
• sialic acid binding Ig-like lectin 1, sialoadhesin
• sialoadhesin
• Siglec1
• Siglec-1

Background

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Siglec-1/CD169 Antibody (NB600-534AF488) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 01/31/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: human lung cancer,Human Spleen
• Species: Human
• Lot: A-4012318-AF488

Bioinformatics

• Gene Symbol: SIGLEC1