CD4 Antibody (RPA-T4) - Azide Free


Flow Cytometry: CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.
Flow Cytometry: CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.
Flow (Cell Surface): CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this more
Flow (Cell Surface): CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. more
Flow (Cell Surface): CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype more
Flow Cytometry: CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.
Flow Cytometry: CD4 Antibody (RPA-T4) [Azide Free] [NBP2-27216] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.

Product Details

Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready, IHC-P (-)
Azide Free
1.0 mg/ml

Order Details

CD4 Antibody (RPA-T4) - Azide Free Summary

PHA-stimulated human PBMC.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Cell Surface)
Application Notes
RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80% of thymocytes and 45% of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.
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NBP2-27216 in the following applications:

Reactivity Notes

This product reacts with Human.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
sterile PBS
No Preservative
1.0 mg/ml
Protein G purified

Alternate Names for CD4 Antibody (RPA-T4) - Azide Free

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4


CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (NBP2-27216)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.

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Showing Publication 1 - 1 of 1.
Publication using NBP2-27216 Applications Species
Hellwinkel JE, Redzic JS, Harland TA et al. Glioma-derived extracellular vesicles selectively suppress immune responses Neuro-oncology (FLOW, Human)

This publication used the Allophycocyanin conjugated form of this antibody (Cat# NBP2-27245).
FLOW Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD4 Antibody (NBP2-27216). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at with any questions regarding this program.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-27216AF405
Alexa Fluor 488 Labeled NBP2-27216AF488
Alexa Fluor 647 Labeled NBP2-27216AF647
Alexa Fluor 700 Labeled NBP2-27216AF700
Allophycocyanin Labeled NBP2-27245
DyLight 350 Labeled NBP2-27216UV
DyLight 405 Labeled NBP2-27216V
DyLight 488 Labeled NBP2-27216G
DyLight 550 Labeled NBP2-27216R
DyLight 650 Labeled NBP2-27216C
DyLight 680 Labeled NBP2-27216FR
DyLight 755 Labeled NBP2-27216IR
HRP Labeled NBP2-27216H
PE Labeled NBP2-27248
PerCP Labeled NBP2-27216PCP

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Blogs on CD4. Showing 1-10 of 16 blog posts - Show all blog posts.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Gene Symbol CD4