Tyrosine Hydroxylase Antibody

Images

 
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal ...read more
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions ...read more
Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Western blot of 10 ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
Immunohistochemistry-Frozen: Tyrosine Hydroxylase Antibody [NB300-109] - 10x Magnification cryo-sections mouse: primary antibody: anti-rb TH: 1:500; o.n. secondary antibody: 1:500 dk-anti-rb DyLight488. IHC-Fr image ...read more
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the mushroom body. All panels show a right brain hemisphere (dorsal to the top, medial to the right), except ...read more
Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Western blot analysis confirms activation of UPR following Hotel protein lysates from the right adrenal medulla of saline (2RS) and RH groups (2RH) were ...read more
Immunohistochemistry Whole-Mount: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical analysis of tyrosine hydroxylase in rat mesenteric artery-whole. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical analysis of a formalin fixed and paraffin embedded rat brain tissue section using Tyrosine Hydroxylase antibody at 1:5000 ...read more
Immunohistochemistry Whole-Mount: Tyrosine Hydroxylase Antibody [NB300-109] - Immunostaining of whole-mount Drosophila brains using NB300-109 at 1:500 dilution. The tyrosine hydroxylase antibody worked really well and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Dr, I, MaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.15 mg/ml

Order Details

Tyrosine Hydroxylase Antibody Summary

Immunogen
This tyrosine hydroxylase antibody is made against SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.
Localization
NB300-109 can be used as a marker for dopaminergic and noradrenergic neurons.
Marker
Neuronal Marker
Specificity
Specific for the ~60 kDa tyrosine hydroxylase protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Dual RNAscope ISH-IHC
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Knockout Validated
Application Notes
Immunohistochemistry: use reported in literature (PMID: 22262884 and PMID: 23811324). Use in rat and mouse reported in customer review. Use in Immunohistochemistry Free floating reported in scientific literature (PMID 25731749). Use in Immunohistochemistry Whole mount reported in scientific literature (PMID: 26123524). Dual ISH-IHC validation (PMID: 29917232). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Fetal Whole Normal)
Recombinant Human Tyrosine Hydroxylase His Protein
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB300-109 in the following applications:

Publications
Read Publications using
NB300-109 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32756440). The tyrosine hydroxylase antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF. Insect reactivity reported in scientific literature (PMID: 27494326)..

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Preservative
No Preservative
Concentration
0.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tyrosine Hydroxylase Antibody

  • anti-TH
  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH mouse
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase immunohistochemistry
  • Tyrosine Hydroxylase mouse
  • Tyrosine Hydroxylase

Background

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1). Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tyrosine Hydroxylase Antibody (NB300-109)(143)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Insect.

We have publications tested in 12 applications: Dual ISH-IHC, ICC/IF, IHC, IHC-F, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, ISH, WB, WB,IHC.


Filter By Application
Dual ISH-IHC
(1)
ICC/IF
(32)
IHC
(43)
IHC-F
(1)
IHC-FR
(2)
IHC-Fr
(6)
IHC-FrFl
(7)
IHC-P
(8)
IHC-WhMt
(3)
ISH
(1)
WB
(20)
WB,IHC
(1)
All Applications
Filter By Species
Human
(19)
Mouse
(69)
Rat
(15)
Drosophila
(10)
Insect
(2)
All Species
Showing Publications 1 - 10 of 143. Show All 143 Publications.
Publications using NB300-109 Applications Species
Wang H, Dou S, Zhu J et al. Ghrelin protects dopaminergic neurons against MPTP neurotoxicity through promoting autophagy and inhibiting endoplasmic reticulum mediated apoptosis Brain Res Jul 26 2020 [PMID: 32710901] (IHC-Fr, WB, Mouse) IHC-Fr, WB Mouse
Davis R B, Ding S et al. Calcitonin-Receptor-Like Receptor Signaling Governs Intestinal Lymphatic Innervation and Lipid Uptake. ACS Pharmacol Transl Sci Dec 4 2019 [PMID: 32219216] (IHC, Mouse) IHC Mouse
Jin JW, Fan X, Del Cid-Pellitero E et al. Development of an alpha-synuclein knockdown peptide and evaluation of its efficacy in Parkinson's disease models Communications biology Feb 19 2021 [PMID: 33608634] (ICC/IF, Rat) ICC/IF Rat
Wang H, Dou S, Zhu J et al. Ghrelin mitigates MPP+-induced cytotoxicity: Involvement of ERK1/2-mediated Nrf2/HO-1 and endoplasmic reticulum stress PERK signaling pathway Peptides Aug 16 2020 [PMID: 32814076] (WB, Human) WB Human
Son Y, Jeong YJ, Shin NR et al. Inhibition of Colony-Stimulating Factor 1 Receptor by PLX3397 Prevents Amyloid Beta Pathology and Rescues Dopaminergic Signaling in Aging 5xFAD Mice Int J Mol Sci Aug 3 2020 [PMID: 32756440] (IHC-P, Human) IHC-P Human
Garcia-Calero E, MartInez-de-la-Torre M, Puelles L A radial histogenetic model of the mouse pallial amygdala Brain Struct Funct Jun 24 2020 [PMID: 32583144] (IHC, Mouse) IHC Mouse
Wu D, Fan Y, Liu S et al. Loading-induced antitumor capability of murine and human urine FASEB J. Apr 15 2020 [PMID: 32293076] (WB, Mouse) WB Mouse
Show All 143 Publications.

Reviews for Tyrosine Hydroxylase Antibody (NB300-109) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Mouse, Rat, Cockroach and Cricket, Other.

Reviews using NB300-109:
Filter by Applications
IHC-Fr
(1)
ICC
(2)
IF
(2)
WB
(1)
All Applications
Filter by Species
Mouse
(3)
Rat
(2)
Cockroach and Cricket
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Tyrosine Hydroxylase NB300-109
Enlarge
5
reviewed by:
michele d'angelo
IHC-Fr Mouse 02/12/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedbrian tissue
SpeciesMouse
Lotajo1215y
 Tyrosine Hydroxylase NB300-109
Enlarge
5
reviewed by:
Bianca Kerschbaumer
Mouse 05/19/2017
View

Summary

Sample TestedBrain (substantia nigra),mouse eye
SpeciesMouse
Lotajo616p

Comments

Commentscryo sections mouse:
primary antibody: anti-rb TH: 1:500; o.n.
secondary antibody: 1:500 dk-anti-rb DyLight488 (Agrisera, AS10 1165)
  4
reviewed by:
Yoshitaka Hamanaka
ICC Cockroach and Cricket 02/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedBrain
SpeciesCockroach and Cricket
Immunocytochemistry Tyrosine Hydroxylase NB300-109
Enlarge
5
reviewed by:
Anonymous
ICC Rat 03/27/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedMesenteric artery-whole
SpeciesRat
LotAJ0314b

Blocking

Blocking Details5% NDS, 1hr at room temperature

Details

Detection NotesConfocal Microscopy, Zeiss Axiovison Observer Z.1 with spinning disk confocal, 20x, PGP9.5-pos/no AB neg
Immunofluorescence Tyrosine Hydroxylase NB300-109
Enlarge
5
reviewed by:
Anonymous
IF Mouse 02/24/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse
CommentsWe find that this NB product does not produce cross-reaction when double-staining for serotonin transporters, whereas a TH antibody from a major vendor does.

Blocking

Blocking Detailssee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Primary Anitbody

Dilution Ratiosee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Secondary Antibody

Secondary Descriptionsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Secondary Manufacturer Cat#see Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Secondary Concentrationsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Details

Fixation Detailssee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Wash Descriptionsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Comments

CommentsWe find that this NB product does not produce cross-reaction when double-staining for serotonin transporters, whereas a TH antibody from a major vendor does.
Western Blot Tyrosine Hydroxylase NB300-109
Enlarge
5
reviewed by:
Naoharu Takano
WB Rat 01/03/2012
View

Summary

ApplicationWestern Blot
Sample TestedPC12 cell whole lysate, Sample Amount: 12ug
SpeciesRat
CommentsThis antibody works well.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4C

Comments

CommentsThis antibody works well.
  5
reviewed by:
Anonymous
IF Other 02/04/2010
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesOther
Lotajo209
Commentscan I get a discount and free shipping on my next purchase of this antibody?

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: 20 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: InVitrogen, Secondary Ab Dilution: 1/200

Comments

Commentscan I get a discount and free shipping on my next purchase of this antibody?

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NB300-109) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

michele d'angelo
02/12/2020
Application: IHC-Fr
Species: Mouse

Bianca Kerschbaumer
05/19/2017
Application:
Species: Mouse

 
Yoshitaka Hamanaka
02/15/2017
Application: ICC
Species: Cockroach and Cricket

Bioinformatics

Gene Symbol TH
Entrez
Uniprot