Tyrosine Hydroxylase Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. Image from confirmed customer review.
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Analysis of Tyrosine Hydroxylase in rat mesenteric artery-whole. Image from verified customer review.
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical staining of retina tissue using TH antibody, NB300-109.
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Immunostaining of whole-mount Drosophila brains using NB300-109 at 1:500 dilution. The tyrosine hydroxylase antibody worked really ...read more
Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Western blot of 10 ug of rat striatal lysate showing specificimmunolabeling of the ~60 kDa tyrosine hydroxylase protein
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/ml of PC-12 lysate. This experiment was performed under reducing conditions ...read more
Immunocytochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - 10x Magnification cryo-sections mouse: primary antibody: anti-rb TH: 1:500; o.n. secondary antibody: 1:500 dk-anti-rb DyLight488. This image was submitted ...read more
Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical analysis of a formalin fixed and paraffin embedded rat brain tissue section using Tyrosine Hydroxylase antibody at 1:5000 ...read more
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Rat midbrain mixed neuronal cultures showing TH positive neurons in green and MAP2 in red. Image courtesy of Aurelie de Rus ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Dr, I, MaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.15 mg/ml

Order Details

Tyrosine Hydroxylase Antibody Summary

Immunogen
SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma
Localization
NB300-109 can be used as a marker for dopaminergic and noradrenergic neurons.
Marker
Neuronal Marker
Specificity
Specific for the ~60k tyrosine hydroxylase protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry 1:500
  • Immunohistochemistry Free-Floating
Application Notes
Immunohistochemistry: use reported in literature (PMID: 22262884 and PMID: 23811324). Use in rat and mouse reported in customer review. Use in Immunohistochemistry-Free floating reported in scientific literature (PMID 25731749) In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Heart Lysate (NB820-59217)
Brain Lysate (NB820-59468)
Tyrosine Hydroxylase Recombinant Protein (NBP1-42461)
Reviewed Applications
Read 6 Reviews rated 4.8
using
NB300-109 in the following applications:

Publications
Read Publications using
NB300-109 in the following applications:

Reactivity Notes

The tyrosine hydroxylase antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF. Insect reactivity reported in scientific literature (PMID: 27494326).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tyrosine Hydroxylase Antibody

  • anti-TH
  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH mouse
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase immunohistochemistry
  • Tyrosine Hydroxylase mouse
  • Tyrosine Hydroxylase

Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and its availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tyrosine Hydroxylase Antibody (NB300-109)(89)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Insect.

We have publications tested in 9 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, WB, WB,IHC.


Filter By Application
ICC/IF
(22)
IHC
(29)
IHC-FR
(2)
IHC-Fr
(6)
IHC-FrFl
(5)
IHC-P
(4)
IHC-WhMt
(1)
WB
(10)
WB,IHC
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(48)
Rat
(10)
Drosophila
(5)
Insect
(1)
All Species
Showing Publications 1 - 10 of 89. Show All 89 Publications.
Publications using NB300-109 Applications Species
Richter S, D'Antongiovanni V, Martinelli S et al. Primary fibroblast co-culture stimulates growth and metabolism in Sdhb-impaired mouse pheochromocytoma MTT cells Cell Tissue Res. Aug 29 2018 [PMID: 30159755] (WB, Mouse) WB Mouse
Hudry E, Andres-Mateos E, Lerner EP et al. Efficient Gene Transfer to the Central Nervous System by Single-Stranded Anc80L65 Mol Ther Methods Clin Dev Sep 21 2018 [PMID: 30109242] (IHC, Mouse) IHC Mouse
kim D, Yoo JM, Hwang H et al. Graphene quantum dots prevent a-synucleinopathy in Parkinson's disease. Nat Nanotechnol. Jul 9 2018 [PMID: 29988049] (IHC, Mouse) IHC Mouse
Bhupal PK, Anderson KA, Shall GP et al. Behavioral and neurochemical responses derived from dopaminergic intrastriatal grafts in hemiparkinsonian rats engaged in a novel motor task J. Neurosci. Methods Jun 17 2018 [PMID: 29924980] (IHC, Rat) IHC Rat
Fielding JW, Hodson EJ, Cheng X et al. PHD2 inactivation in Type I cells drives HIF-2alpha dependent multi-lineage hyperplasia and the formation of paraganglioma-like carotid bodies J. Physiol. (Lond.) Jun 19 2018 [PMID: 29917232] (Mouse) Mouse
Lee S, Kim S, Park YJ et al. The c-Abl inhibitor, Radotinib HCl, is neuroprotective in a preclinical Parkinson's disease mouse model Hum. Mol. Genet. Jul 1 2018 [PMID: 29897434] (ICC/IF, Mouse) ICC/IF Mouse
Williams ET, Glauser L, Tsika E et al. Parkin mediates the ubiquitination of VPS35 and modulates retromer-dependent endosomal sorting Hum. Mol. Genet. Jun 11 2018 [PMID: 29893854] (Human) Human
Je G, Guhathakurta S, Yun SP et al. A novel extended form of alpha-synuclein 3'UTR in the human brain Mol Brain May 25 2018 [PMID: 29801501] (WB, Mouse) WB Mouse
Kim D, Hwang H, Choi S et al. D409H GBA1 mutation accelerates the progression of pathology in A53T a-synuclein transgenic mouse model. Acta Neuropathol Commun Apr 27 2018 [PMID: 29703245] (Mouse) Mouse
Sun J, Xu AQ, Giraud J et al. Neural Control of Startle-Induced Locomotion by the Mushroom Bodies and Associated Neurons in Drosophila. Front Syst Neurosci Mar 28 2018 [PMID: 29643770] (IHC, Drosophila) IHC Drosophila
Show All 89 Publications.

Reviews for Tyrosine Hydroxylase Antibody (NB300-109) (6) 4.86

Average Rating: 4.8
(Based on 6 reviews)
We have 6 reviews tested in 4 species: Mouse, Rat, Cockroach and Cricket, Other.

Reviews using NB300-109:
Filter by Applications
ICC
(2)
IF
(2)
WB
(1)
All Applications
Filter by Species
Mouse
(2)
Rat
(2)
Cockroach and Cricket
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 Tyrosine Hydroxylase NB300-109
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reviewed by:
Bianca Kerschbaumer
Mouse 05/19/2017
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Summary

Sample TestedBrain (substantia nigra),mouse eye
SpeciesMouse
Lotajo616p

Comments

Commentscryo sections mouse:
primary antibody: anti-rb TH: 1:500; o.n.
secondary antibody: 1:500 dk-anti-rb DyLight488 (Agrisera, AS10 1165)
 
reviewed by:
Yoshitaka Hamanaka
ICC Cockroach and Cricket 02/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedBrain
SpeciesCockroach and Cricket
Immunocytochemistry Tyrosine Hydroxylase NB300-109
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reviewed by:
Anonymous
ICC Rat 03/27/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedMesenteric artery-whole
SpeciesRat
LotAJ0314b

Blocking

Blocking Details5% NDS, 1hr at room temperature

Details

Detection NotesConfocal Microscopy, Zeiss Axiovison Observer Z.1 with spinning disk confocal, 20x, PGP9.5-pos/no AB neg
Immunofluorescence Tyrosine Hydroxylase NB300-109
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reviewed by:
Anonymous
IF Mouse 02/24/2014
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse
CommentsWe find that this NB product does not produce cross-reaction when double-staining for serotonin transporters, whereas a TH antibody from a major vendor does.

Blocking

Blocking Detailssee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Primary Anitbody

Dilution Ratiosee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Secondary Antibody

Secondary Descriptionsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Secondary Manufacturer Cat#see Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Secondary Concentrationsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Details

Fixation Detailssee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.
Wash Descriptionsee Wei W et al. (2013) J Neurophysiol. 110:2203-2216.

Comments

CommentsWe find that this NB product does not produce cross-reaction when double-staining for serotonin transporters, whereas a TH antibody from a major vendor does.
Western Blot Tyrosine Hydroxylase NB300-109
Enlarge
reviewed by:
Naoharu Takano
WB Rat 01/03/2012
View

Summary

ApplicationWestern Blot
Sample TestedPC12 cell whole lysate, Sample Amount: 12ug
SpeciesRat
CommentsThis antibody works well.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4C

Comments

CommentsThis antibody works well.
 
reviewed by:
Anonymous
IF Other 02/04/2010
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesOther
Lotajo209
Commentscan I get a discount and free shipping on my next purchase of this antibody?

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: 20 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: InVitrogen, Secondary Ab Dilution: 1/200

Comments

Commentscan I get a discount and free shipping on my next purchase of this antibody?

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NB300-109) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tyrosine Hydroxylase Antibody (NB300-109)

Discover related pathways, diseases and genes to Tyrosine Hydroxylase Antibody (NB300-109). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Tyrosine Hydroxylase Antibody (NB300-109)

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Research Areas for Tyrosine Hydroxylase Antibody (NB300-109)

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Recent Reviews

4.8
5
5
4
1
3
0
2
0
1
0

Bianca Kerschbaumer
05/19/2017
Application:
Species: Mouse

 
Yoshitaka Hamanaka
02/15/2017
Application: ICC
Species: Cockroach and Cricket

Anonymous
03/27/2015
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TH
Entrez
Uniprot