Acetylcholinesterase/ACHE Antibody

Images

 
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AChE stained by AChE ...read more
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse brain. AChE stained by AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated ...read more
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse colon. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat fore brain. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat brain. AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Frozen sectioned E13.5 Rat brain. Green: AChE protein stained by AChE antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, Gp, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Acetylcholinesterase/ACHE Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.
Localization
Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, Isoform T: Nucleus, Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Cell junction, synapse, Secreted, Cell membrane, Isoform T: Nucleus, Isof
Marker
Early Neuronal Development Marker
Predicted Species
Rhesus Macaque (99%), Feline (95%), Bovine (94%), Guinea Pig (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACHE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP1-30052
Species: Ch, Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-13306
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-86615
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP2-80461
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP2-48480
Species: Hu
Applications: Flow-CS, Flow, IHC, IHC-Fr, IHC-P
H00010908-M08
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-46349
Species: Hu
Applications: IHC, WB
256-GF/CF
Species: Hu
Applications: BA

Publications for Acetylcholinesterase/ACHE Antibody (NBP1-31329) (0)

There are no publications for Acetylcholinesterase/ACHE Antibody (NBP1-31329).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Acetylcholinesterase/ACHE Antibody (NBP1-31329) (0)

There are no reviews for Acetylcholinesterase/ACHE Antibody (NBP1-31329). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Acetylcholinesterase/ACHE Antibody (NBP1-31329). (Showing 1 - 1 of 1 FAQ).

  1. We recently purchased your Acetylcholinesterase/ACHE Antibody (NBP1-31329) and Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (NB7160) for western blot. What buffer do you recommend for diluting these particular antibodies and what is the recommended blocking buffer?
    • Acetylcholinesterase/ACHE Antibody #NBP1-31329 is already in PBS and 20% glycerol.  Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] #NB7160 is in PBS and 0.2% BSA.  You can use PBS for diluting either one of them. We do not have a specific protocol for these antibodies. Our standard in house blocking buffer is TBST with 5% non-fat dry milk. You may refer to our general protocol for any more details or tips:   https://www.novusbio.com/support/support-by-application/western-blot/protocol.html

Secondary Antibodies

 

Isotype Controls

Additional Acetylcholinesterase/ACHE Products

Bioinformatics Tool for Acetylcholinesterase/ACHE Antibody (NBP1-31329)

Discover related pathways, diseases and genes to Acetylcholinesterase/ACHE Antibody (NBP1-31329). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Acetylcholinesterase/ACHE Antibody (NBP1-31329)

Discover more about diseases related to Acetylcholinesterase/ACHE Antibody (NBP1-31329).
 

Pathways for Acetylcholinesterase/ACHE Antibody (NBP1-31329)

View related products by pathway.

PTMs for Acetylcholinesterase/ACHE Antibody (NBP1-31329)

Learn more about PTMs related to Acetylcholinesterase/ACHE Antibody (NBP1-31329).
 

Research Areas for Acetylcholinesterase/ACHE Antibody (NBP1-31329)

Find related products by research area.

Blogs on Acetylcholinesterase/ACHE.

HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins
Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Acetylcholinesterase/ACHE Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ACHE
Entrez
Uniprot