Tyrosine Hydroxylase: Novus Biologicals

NB300-109

Tyrosine Hydroxylase Antibody - Azide Free

(7 Reviews)

(252 Publications)

0.1 ml

NB300-109

Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.

Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western blot of 10 ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

10x Magnification cryo-sections mouse: primary antibody: anti-rb TH: 1:500; o.n. secondary antibody: 1:500 dk-anti-rb DyLight488. IHC-Fr image submitted by a verified customer review.

Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NB300-109-img0028.jpg

Tyrosine-Hydroxylase-Antibody-Western-Blot-NB300-109-img0030.jpg

Tyrosine Hydroxylase Antibody-Immunohistochemistry-NB300-109-img0031.jpg

Immunohistochemical analysis of a formalin fixed and paraffin embedded rat brain tissue section using Tyrosine Hydroxylase antibody at 1:5000 dilution. The primary antibody binding to its antigen was detected using HRP anti-Polyvalent ready-to-use kit (Ultratek, 35368) with DAB (brown) and the sections were further counterstained using hematoxylin. Isotype control section was incubated with Rabbit IgG Isotype Control antibody and was processed under the same assay conditions. Tyrosine hydroxylase staining (brown) was observed in the test samples only and the signal was specifically localized to the neuronal cells.

Immunostaining of whole-mount Drosophila brains using NB300-109 at 1:500 dilution. The tyrosine hydroxylase antibody worked really well and produced a bright staining with almost no background. Data courtesy of Dr. Olga Alekseenko, Neurobiology Dept. Harvard Medical School.

Immunohistochemical analysis of tyrosine hydroxylase in rat mesenteric artery-whole. ICC/IF image submitted by a verified customer review.

Western blot analysis confirms activation of UPR following RH.Total protein lysates from the right adrenal medulla of saline (2RS) and RH groups (2RH) were subjected to Western blot analyses as described in methods. Proteins were separated by SDS-PAGE, electroblotted and the membranes were sequentially probed with antibodies specific to Grp78, Derlin1, TH and GAPDH. Similar results were obtained in two separate replicate experiments.

Image showing differentiation of neurospheres as evidenced morphologically (A) and through staining by Tyrosine Hydroxylase (B) to show the ability to derive dopaminergic neurons from the rat progenitor cells.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - D409H GBA1 expression promotes reduction of dopaminergic fiber densities & motor deficit in the striatum of hA53T alpha -synuclein transgenic mice. a, The striatal TH-immunopositive fiber density was assessed in the striatum of the indicated genotypes at 6 months of age (n = 6 per each group). The scale bar is 250 μm. b, The optical densities of TH positive signals were quantified. c & d, Rotarod & Pole test were assessed at 6 months of age with the indicated genotypes (n = 7 per each group). e, the body weight of mice were measured with the indicated genotypes (n = 15 per each group). DATA are expressed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0538-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Dysregulation of RNF146-PARP1 pathways in PD pathogenesis. (C) Expression of PARsylated proteins, RNF146, TH & phosphorylated alpha -synuclein (pS129-a-syn) in postmortem striatum from PD patients & age-matched control subjects (Ctrl) determined by WBs using designated antibodies. Image collected & cropped by CiteAb from following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21828), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Image showing differentiation of neurospheres as evidenced morphologically (A) & through staining by Tyrosine Hydroxylase (B) to show the ability to derive dopaminergic neurons from the rat progenitor cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30042680), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Immunoblots of tyrosine hydroxylase in the cricket & cockroach brain.Protein extracts (8 μl & 16 μl) from the male cricket, Gryllus bimaculatus & male cockroach, Periplaneta americana were electrophoresed, transferred to a membrane & reacted with anti-tyrosine hydroxylase (TH). The anti-TH recognized a single band of protein of ~ 66 kDa (arrow heads) in both the cricket (left two lanes) & cockroach (right two lanes). M, molecular marker. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - D409H GBA1 expression shortens lifespan & leads to dopaminergic degeneration in the hA53T alpha -synuclein transgenic mice. a, Survival was monitored from littermates with the following genotypes: hA53Ta-Syn/GBA1+/+ (n = 15), hA53T alpha -Syn/GBA1+/D409H(n = 16), hA53T alpha -Syn/GBA1D409H/D409H (n = 18) mice. GBA1 mutation induces the lethal phenotype & TH-positive neuronal loss in the SNpc of A53T mutant alpha -synuclein transgenic mice. b & c, The number of TH-positive neurons in the SNpc was counted using stereological analysis with the indicated genotypes at 6 months of age. The scale bar is 200 μm. DATA are expressed as mean ± SEM (n = 6 for the each group) Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0538-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the deutocerebrum & medial antennal lobe tract.Tyrosine hydroxylase (TH) immunoreactivity is shown in magenta (A, D, G, J) & synapsin immunoreactivity in green (B, E, H, K). Corresponding merged images (C, F, I, L). A-C: Frontal sections through the antennal lobe (AL). Insets indicate single optical slice ( 0.05. b Heatmaps of proteins significantly enriched under toxic stress (10 µM CCCP; 20 nM rotenone) & their validation by western blot (Input, total levels & S-HSP90 bait isolate, chaperome network associated). Mean ± SEM, n = 3 individual values from the different experiments shown as points, t-test, ***p < 0.001; *p < 0.05. c Western blot & quantification of rotenone stress increase in the p-TH:TH ratio & its reduction by PU-H71 (200 nM). p-ERK/ERK, cell viability control; beta -actin, protein loading control. Mean ± SEM, n = 3 individual values from the different experiments shown as points, One-way ANOVA with Tukey’s post-hoc, ***p < 0.001; **p < 0.01. d Total intracellular dopamine levels in PD mDA neurons in conditions of toxic stress & under PU-H71 rescue; Mean ± SEM, n = 4–5 individual values from the different experiments shown as points, t-test, *p < 0.05. e PU-H71 treatment of rotenone- or CCCP-stressed mDA neurons significantly increases their viability, as measured by total ATP levels; means ± SEM, n = 3–8 from independent differentiations, t-test, ***p < 0.001; **p < 0.01; *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - ErbB4 protein expression in mesencephalic nuclei.The overview on the mesencephalon (B) shows dense ErbB4 expression (mAb-10, green channel) in the dopaminergic nuclei SNc & VTA that is clearly above background levels, whereas expression in the red nucleus is weaker. Higher magnification shows abundant immunoreactivity on neuropil (C–E, C'–E'). At 100x magnification, a dense network of ErbB4-positive neuropil surrounding a neuron (asterisk) is evident in the VTA (E”). The red channel shows neurofilament H-immunofluorescence in panels (B–E) & TH-immunofluorescence in (F–G). Co-immunolabeling reveals that subsets of TH-positive dopaminergic somata express ErbB4 in the VTA (F) & SNc (G); coexpressing neurons are indicated by arrowheads (F–G), whereas lack of ErbB4 signal is indicated by arrows (G). The location of the overview image within the monkey brain is indicated by a red rectangle in (A). The location of the profiles through mesencephalic nuclei is indicated by yellow boxes in (A) & by rectangles in (B). Images were obtained with a confocal microscope. Scale bar = 3 mm (B), 400 µm (C–E), 175 µm (C'–E', F–G), 70 µm (E”). Abbreviations: cerebral peduncle (cp), oculomotor nerve (3n), substantia nigra pars reticulata (SNr). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0027337), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in mushroom body.All panels show a right brain hemisphere, except panels M-O (posterior to top). Tyrosine hydroxylase (TH) immunoreactivity shown in magenta (A, D, G, J, M) & synapsin immunoreactivity (green: B, E, H, K, N). Corresponding merged images in C, F, I, L, O. A-C: Frontal sections through lateral protocerebrum. 2 groups of TH-positive cell bodies (DCa1 & 2) labeled. DCa2 appears to send axons ventrally (arrowhead). Superior lateral protocerebrum (SLP) as well as medial & lateral calyces (MCA & LCA) innervated by TH-positive fibers. D-F: Frontal sections through vertical lobe (VL) & parts of medial calyx (MCA), which receive many fine TH-positive fibers. G-I: Horizontal sections through a tip of vertical lobe & dorsal parts of calyces. Both calyces & vertical lobe innervated by fibrous TH-positive fibers. Note TH-immunoreactive fibers exclusively innervate synapsin-positive neuropil layers (NLs) of calyx where dendrites of Kenyon cells receive synaptic inputs from antennal-lobe projection neurons. J-L: Horizontal sections through calyces, ventral to panels G-I. Here, 3clusters of TH-positive cell bodies (DCa1, DCa2, & DSP1) visible. Immunoreactive fibers mainly invade anterior layers including gamma layer ( gamma ) in vertical lobe. M-O: Horizontal sections through distal portions of medial lobes (MLs), invaded by fine TH-positive fibers exhibiting moderate immunolabeling (arrows). in inferior medial protocerebrum (IMP) anterior to medial lobe, a pair of DIP1 clusters (triangles) is situated. D, dorsal; KFL, Kenyon fiber layer; LCA, lateral calyx; LO, lobula; M, medial; P, posterior; PED, pedunculus; SMP, superior medial protocerebrum. Scale bars = 100 μm. Image collected & cropped by CiteAb from following publication (//dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Characterization of the nigrostriatal pathway of LRRK2 conditional transgenic mice. A, Representative TH immunohistochemistry of the midbrain coronal sections of 22-month-old LRRK2 GS & GSDA transgenic & age-matched littermate controls. B, C, Stereological assessment of TH-positive (B) & Nissl-positive (C) neurons in the SNpc (control, n = 9; GS, n = 9; GS/DA, n = 9). Data are the mean number of cells per region ± SEM, n = 9 mice per group. Statistical significance was determined by two-tailed unpaired Student’s t test. D, Representative images of TH immunostaining of nerve terminals in the striatum of LRRK2 conditional transgenic mice at 22 months of age. E, Quantitation of TH immunostaining in the striatum using ImageJ software (NIH; control, n = 7; GS, n = 7; GS/DA, n = 7). Differences between groups were assessed by two-way ANOVA. Bars represent the mean ± SEM (n ≥ 5 animals/genotype). n.s., Nonsignificant. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0004-17.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ch, Dr, I, Ma, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Dr, I, Ma, Ch

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NB300-109-25ul

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NB300-110

Tyrosine Hydroxylase Antibody - Azide Free

(5 Reviews)

(104 Publications)

0.1 ml

NB300-110

Tyrosine Hydroxylase Antibody-Immunocytochemistry-Immunofluorescence-NB300-110-img0013.jpg

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-110] - Mouse cryo-sections. Antibody at 1:500. Secondary antibody: dk-anti-sp Alexa Fluor488 at 1:500; 1 h. IHC image submitted by a verified customer review.

Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NB300-110-img0012.jpg

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NB300-110] - Sagittal section of Pleurodeles waltl (amphibian) brain. The cell bodies of dopaminergic neurons in ventral tegmental area and dopaminergic fibers in striatum are stained with the TH antibody. IHC image submitted by a verified customer review.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-110] - Immunohistochemistry analysis of gelatin section of mouse brain (substantia nigra pars compacta) using Tyrosine Hydroxylase antibody. IHC image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Activation of the DDR in mice transduced with AAV2/6 h-syn.a h-syn expression increases 53BP1 & gamma H2AX foci, & ATM phosphorylation in nigral dopaminergic neurons. b The DDR is not activated by viral delivery of GFP. Scale bar: 50 μm. (**p 99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Developmental song exposure & social context affect cFOS expression in TH neurons of the caudal VTA. Sagittal drawings (A,C) & photomicrographs of tyrosine hydroxylase (TH; green label) expression (B,D) in the ventral tegmental area (VTA) & periaqueductal gray (PAG; panels A,B), & the substantia nigra pars compacta (SNc) & locus coeruleus (LC; panels C,D). (E) Examples of co-localized expression of TH (green) & cFOS (red) in the caudal VTA of a normally-reared bird that heard silence (left panel), UD song (middle panel; UD song) & FD song (FD; right panel). White arrows indicate examples of colocalized expression. (F) Percent of TH neurons co-localized with cFOS in the caudal VTA (cVTA), rostral VTA (rVTA) & SNc. Box-and-whisker plots for normally-reared (yellow colors) & song-naïve (green colors) hearing UD (UD; lighter colors) or female-directed (FD; darker colors) songs. Each box spans the interquartile range, horizontal white lines indicate the median & whiskers show the minima & maxima. Levels of cFOS expression in TH neurons for silence controls are plotted as the mean (gray dashed line) +/− standard error (gray boxes). *Indicates a significant difference at p 99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Conversion of hNES cells into hPRogFPM & their differentiation into midbrain dopaminergic neurons. a Schematic representation of the conversion & differentiation protocols. b, cRT-qPCR analysis at day 8, showing the expression of midbrain-hindbrain TFs, such as OTX2, GBX2, LMX1A, & FOXA2 (b), as well as the dopaminergic neuron markers, NR4A2, TH, SLC18A22, & SLC6A3 (c). d Immunocytochemistry analysis of the presence of OTX2 & TH in control unconverted NES cultures, compared with NES cells converted with SAF+Dkk1 & differentiated until day 8. e, f Percentage of OTX2+ & TH+ cells in the conditions in d. P = 0.02673 (e), P = 0.03233 (f), n = 3. g–i Expression of the key midbrain TFs, LMX1A, NR4A2, & PBX1, in TH+cells derived from SAI2-NES cells after conversion & differentiation. j, k TH+ cells express the mature neuronal marker, MAP2 (j), & some acquire mature neuronal morphologies, with long processes & varicosities at day 8 (k). Scale 50μm. Box plots (b, c, e, f): Center line, median; hinges, 25% & 75% quartiles; whiskers, 1.5 interquartile range. Statistics: (b, c) ANOVA, followed by pair-wise t-test with Bonferroni correction for multiple testing. (e, f). Two sample t-test; *P ≤ 0,05; **P ≤ 0,01; ***P ≤ 0,001. N = 3 (GBX2, FOXA2, TH, SLC6A3), n = 4 (LMX1A, OTX2, NR4A2, SLC18A2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29968757), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Comparison of Mouse & Human Dopaminergic Neuronal Development(A) WNT1 compartments marking lateral population of the floor plate in human & mouse tissue (scale bar, 100 μm).(B) Bar plot of cell types of the human & mouse dopaminergic lineage, showing the expression of key genes. Bars show average mRNA expression, scaled to the absolute molecule counts indicated on the right axis. Error bars show SEM.(C) Validation of mNbM by in situ hybridization for Igfbpl1 & Nhlh1 (scale bar, 50 μm).(D) Neuroblasts in human & mouse ventral midbrain (scale bar, 100 μm; magnification, 20 μm).(E) Selected genes showing similar (left) or distinct (right) expression in mouse & human ventral midbrain. Blue, expressed above baseline in mouse (>99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - alpha -Synuclein overexpression or intracerebral seeding impacts the dopaminergic system.a–c Intranigral injection of AAV2/6 serotype expressing human alpha -syn (h-syn) results in increased protein expression paralleled by reduction in tyrosine hydroxylase (TH) levels. (d) h-syn is also expressed in dopaminergic cell bodies (arrows). e Unbiased stereological counts demonstrate a reduction in nigral dopaminergic cell bodies. f, g Intracranial injection of alpha -syn pre-formed fibrils (PFF) causes striatal dopaminergic denervation as evidenced by a reduction in TH immunoreactivity. h Increased levels of ser129-phosphosynuclein in the substantia nigra (arrows) indicate alpha -syn stress in PFF injected animals. i Unbiased stereological counts showing a decrease in dopaminergic cell bodies in the substantia nigra. Scale bars: 1 mm in a, b 50 μm in d. (**p < 0.01; ***p < 0.001; Student’s t test). All bar graphs show mean + /− s.e.m Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30050065), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Am, Av, Ma, Rb, Re, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Am, Av, Ma, Rb, Re, Ch

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Alt Catalog#

IMG-5070

Alt Catalog# IMG-5070

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NB300-110SS

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MAB7566

Tyrosine Hydroxylase Antibody (779427)

(1 Review)

(20 Publications)

100 μg

MAB7566

HAF018). Specific bands were detected for Tyrosine Hydroxylase at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). Specific bands were detected for Tyrosine Hydroxylase at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and rat adrenal gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Tyrosine Hydroxylase at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007). Cells were double stained using the Northern-Lights™ 637-conjugated Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (white; Catalog # NL1195V). Cells were counterstained with DAPI (blue). Specific staining of Tyrosine Hydroxylase was localized to cytoplasm of dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Tyrosine Hydroxylase was detected in immersion fixed mouse embryonic stem cells differentiated into dopaminergic neurons using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007). Cells were double stained using the Northern-Lights™ 637-conjugated Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (white; Catalog # NL1195V). Cells were counterstained with DAPI (blue). Specific staining of Tyrosine Hydroxylase was localized to cytoplasm of dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Tyrosine Hydroxylase was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human adrenal gland tissue and mouse adrenal gland tissue, loaded at 0.2 mg/mL. Specific bands were detected for Tyrosine Hydroxylase at approximately 52-61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (Catalog # MAB7566). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.</PP>Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7566U-100UG
Alexa Fluor 405	FAB7566V-100UG
Alexa Fluor 488	FAB7566G-100UG
Alexa Fluor 532	FAB7566X-100UG
Alexa Fluor 594	FAB7566T-100UG
Alexa Fluor 647	FAB7566R-100UG
Alexa Fluor 700	FAB7566N-100UG
Alexa Fluor 750	FAB7566S-100UG
Alexa Fluor Plus 405	FAB7566AFP405-100UG
Alexa Fluor Plus 488	FAB7566AFP488-100UG
Alexa Fluor Plus 555	FAB7566AFP555-100UG
Alexa Fluor Plus 594	FAB7566AFP594-100UG
Alexa Fluor Plus 647	FAB7566AFP647-100UG
Alexa Fluor Plus 680	FAB7566AFP680-100UG
Allophycocyanin	FAB7566A
Biotin	FAB7566B
CoraFluor 1	FAB7566CL1
DyLight 350	FAB7566D
DyLight 405	FAB7566E
DyLight 488	FAB7566K
DyLight 550	FAB7566L
DyLight 594	FAB7566M
DyLight 650	FAB7566W
DyLight 680	FAB7566Y
DyLight 755	FAB7566Z
FITC	FAB7566F
HRP	FAB7566H
Janelia Fluor 525	FAB7566JF525
Janelia Fluor 549	FAB7566I
Janelia Fluor 646	FAB7566J
Janelia Fluor 669	FAB7566JF669
PE	FAB7566P
PerCP	FAB7566C
mFluor Violet 450 SE	FAB7566MFV450
mFluor Violet 500 SE	FAB7566MFV500
mFluor Violet 610 SE	FAB7566MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7566-SP

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NB300-173

Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

(1 Review)

(10 Publications)

0.1 ml

NB300-173

Reactivity:

Mu, Rt, Ma

Species Glossary

Reactivity: Mu, Rt, Ma

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-05555

Tyrosine Hydroxylase Antibody - BSA Free

(1 Publication)

100 ul

NBP3-05555

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-05555-50ul

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NB300-211

Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

(8 Publications)

0.1 ml

NB300-211

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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AF7566

Tyrosine Hydroxylase Antibody [Unconjugated]

(1 Publication)

100 μg

AF7566

Western blot shows lysates of human adrenal gland tissue, mouse adrenal gland tissue, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Tyrosine Hydroxylase at approximately 56-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tyrosine Hydroxylase was detected in immersion fixed D3 mouse embryonic stem cells using Sheep Anti-Human Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) at 10 µg/mL for 3 hours at room temperature. Cells were differentiated into dopaminergic neurons using R&D Systems Human/Mouse Dopaminergic Neuron Differentiation Kit (SC001B). Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to dopaminergic neurons. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Tyrosine Hydroxylase was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human/Mouse/Rat Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of PC-12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tyrosine Hydroxylase at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat Tyrosine Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7566) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7566U-100UG
Alexa Fluor 405	AF7566V-100UG
Alexa Fluor 488	AF7566G-100UG
Alexa Fluor 532	AF7566X-100UG
Alexa Fluor 594	AF7566T-100UG
Alexa Fluor 647	AF7566R-100UG
Alexa Fluor 700	AF7566N-100UG
Alexa Fluor 750	AF7566S-100UG
Alexa Fluor Plus 405	AF7566AFP405-100UG
Alexa Fluor Plus 488	AF7566AFP488-100UG
Alexa Fluor Plus 555	AF7566AFP555-100UG
Alexa Fluor Plus 594	AF7566AFP594-100UG
Alexa Fluor Plus 647	AF7566AFP647-100UG
Alexa Fluor Plus 680	AF7566AFP680-100UG
Allophycocyanin	AF7566A
Biotin	AF7566B
CoraFluor 1	AF7566CL1
DyLight 350	AF7566D
DyLight 405	AF7566E
DyLight 488	AF7566K
DyLight 550	AF7566L
DyLight 594	AF7566M
DyLight 650	AF7566W
DyLight 680	AF7566Y
DyLight 755	AF7566Z
FITC	AF7566F
HRP	AF7566H
Janelia Fluor 525	AF7566JF525
Janelia Fluor 549	AF7566I
Janelia Fluor 646	AF7566J
Janelia Fluor 669	AF7566JF669
PE	AF7566P
PerCP	AF7566C
mFluor Violet 450 SE	AF7566MFV450
mFluor Violet 500 SE	AF7566MFV500
mFluor Violet 610 SE	AF7566MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7566-SP

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NBP3-06921

Mouse Tyrosine Hydroxylase ELISA Kit (Colorimetric)

1 Kit

NBP3-06921

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.16 - 10 ng/mL

Sensitivity:

0.09 ng/mL

Sample Type:

Serum, Plasma, Cell supernatant

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NBP2-46647

Tyrosine Hydroxylase Antibody (CL3049) - BSA Free

(1 Publication)

0.1 ml

NBP2-46647

Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Staining of human prostate shows strong immunoreactivity in sympathetic fibers.

Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Analysis of human kidney shows absence of immunoreactivity (negative control).

Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Immunohistochemical staining of rat locus coeruleus using Tyrosine Hydroxylase antibody (clone 3049) with immunofluorescence based detection showing a very strong cytoplasmic immunoreactivity in monoaminergic neurons.

Immunohistochemistry: Tyrosine Hydroxylase Antibody (CL3049) [NBP2-46647] - Staining of rat locus coeruleus shows strong positivity in noradrenergic neurons.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44498AF350
Alexa Fluor 405	NBP3-44498AF405
Alexa Fluor 488	NBP3-44498AF488
Alexa Fluor 532	NBP3-44498AF532
Alexa Fluor 594	NBP3-44498AF594
Alexa Fluor 647	NBP3-44498AF647
Alexa Fluor 700	NBP3-44498AF700
Alexa Fluor 750	NBP3-44498AF750
Alexa Fluor Plus 405	NBP3-44498AFP405
Alexa Fluor Plus 488	NBP3-44498AFP488
Alexa Fluor Plus 555	NBP3-44498AFP555
Alexa Fluor Plus 594	NBP3-44498AFP594
Alexa Fluor Plus 647	NBP3-44498AFP647
Alexa Fluor Plus 680	NBP3-44498AFP680
Allophycocyanin	NBP3-44498APC
Biotin	NBP3-44498B
CoraFluor 1	NBP3-44498CL1
DyLight 350	NBP3-44498UV
DyLight 405	NBP3-44498V
DyLight 488	NBP3-44498G
DyLight 550	NBP3-44498R
DyLight 594	NBP3-44498DL594
DyLight 650	NBP3-44498C
DyLight 680	NBP3-44498FR
DyLight 755	NBP3-44498IR
FITC	NBP3-44498F
HRP	NBP3-44498H
Janelia Fluor 525	NBP3-44498JF525
Janelia Fluor 549	NBP3-44498JF549
Janelia Fluor 646	NBP3-44498JF646
Janelia Fluor 669	NBP3-44498JF669
PE	NBP3-44498PE
PerCP	NBP3-44498PCP
mFluor Violet 450 SE	NBP3-44498MFV450
mFluor Violet 500 SE	NBP3-44498MFV500
mFluor Violet 610 SE	NBP3-44498MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44498
BSA Free	NBP2-46647-25ul

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NBP3-44864

Tyrosine Hydroxylase Antibody (OTI1A4)

100 ul

NBP3-44864

Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI1A4)[NBP3-44864]

Anti-TH mouse monoclonal antibody immunofluoreaining of COS7 cells transiently transfected by pCMV6-ENTRY TH .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TH mouse monoclonal antibody. . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120Â°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TH (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TH.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TH mouse monoclonal antibody. . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120Â°C for 3 min.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44864AF350
Alexa Fluor 405	NBP3-44864AF405
Alexa Fluor 488	NBP3-44864AF488
Alexa Fluor 532	NBP3-44864AF532
Alexa Fluor 594	NBP3-44864AF594
Alexa Fluor 647	NBP3-44864AF647
Alexa Fluor 700	NBP3-44864AF700
Alexa Fluor 750	NBP3-44864AF750
Allophycocyanin	NBP3-44864APC
Biotin	NBP3-44864B
CoraFluor 1	NBP3-44864CL1
DyLight 350	NBP3-44864UV
DyLight 405	NBP3-44864V
DyLight 488	NBP3-44864G
DyLight 550	NBP3-44864R
DyLight 594	NBP3-44864DL594
DyLight 650	NBP3-44864C
DyLight 680	NBP3-44864FR
DyLight 755	NBP3-44864IR
FITC	NBP3-44864F
HRP	NBP3-44864H
Janelia Fluor 525	NBP3-44864JF525
Janelia Fluor 549	NBP3-44864JF549
Janelia Fluor 646	NBP3-44864JF646
Janelia Fluor 669	NBP3-44864JF669
PE	NBP3-44864PE
PerCP	NBP3-44864PCP
mFluor Violet 450 SE	NBP3-44864MFV450
mFluor Violet 500 SE	NBP3-44864MFV500
mFluor Violet 610 SE	NBP3-44864MFV610

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NBP3-44862

Tyrosine Hydroxylase Antibody (OTI2D4)

100 ul

NBP3-44862

Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TH mouse monoclonal antibody. . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120Â°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TH mouse monoclonal antibody. . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120Â°C for 3 min.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44862AF350
Alexa Fluor 405	NBP3-44862AF405
Alexa Fluor 488	NBP3-44862AF488
Alexa Fluor 532	NBP3-44862AF532
Alexa Fluor 594	NBP3-44862AF594
Alexa Fluor 647	NBP3-44862AF647
Alexa Fluor 700	NBP3-44862AF700
Alexa Fluor 750	NBP3-44862AF750
Allophycocyanin	NBP3-44862APC
Biotin	NBP3-44862B
CoraFluor 1	NBP3-44862CL1
DyLight 350	NBP3-44862UV
DyLight 405	NBP3-44862V
DyLight 488	NBP3-44862G
DyLight 550	NBP3-44862R
DyLight 594	NBP3-44862DL594
DyLight 650	NBP3-44862C
DyLight 680	NBP3-44862FR
DyLight 755	NBP3-44862IR
FITC	NBP3-44862F
HRP	NBP3-44862H
Janelia Fluor 525	NBP3-44862JF525
Janelia Fluor 549	NBP3-44862JF549
Janelia Fluor 646	NBP3-44862JF646
Janelia Fluor 669	NBP3-44862JF669
PE	NBP3-44862PE
PerCP	NBP3-44862PCP
mFluor Violet 450 SE	NBP3-44862MFV450
mFluor Violet 500 SE	NBP3-44862MFV500
mFluor Violet 610 SE	NBP3-44862MFV610

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NBP2-38903

Tyrosine Hydroxylase Antibody - BSA Free

0.1 ml

NBP2-38903

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human caudate shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human stomach shows no positivity in glandular cells as expected.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse amygdala shows positive processes.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse caudate putamen shows synaptic immunoreactivity.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse hindbrain shows positivity in the locus coeruleus.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-38903-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

TH Products By Gene Name

Tyrosine Hydroxylase Antibody - Azide Free

Tyrosine Hydroxylase Antibody - Azide Free

Tyrosine Hydroxylase Antibody (779427)

Tyrosine Hydroxylase [p Ser40] Antibody - Azide Free

Tyrosine Hydroxylase Antibody - BSA Free

Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Tyrosine Hydroxylase Antibody [Unconjugated]

Mouse Tyrosine Hydroxylase ELISA Kit (Colorimetric)

Tyrosine Hydroxylase Antibody (CL3049) - BSA Free

Tyrosine Hydroxylase Antibody (OTI1A4)

Tyrosine Hydroxylase Antibody (OTI2D4)

Tyrosine Hydroxylase Antibody - BSA Free